Streptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/57724 |
Resumo: | Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Laboratório de Ultraestrutura, Recife, PE, Brazil. Universidade Federal de Pernambuco, Departamento de Antibióticos, Recife, Pernambuco, Brasil. Universidade Federal de Pernambuco, Departamento de Química Fundamental, Recife, Pernambuco, Brasil. Universidade Federal de Sergipe, Departamento de Química, Aracaju, Sergipe, Brasil. Universidade Federal de Pernambuco, Departamento de Ciências Farmacêuticas, Laboratório de Química Teórica Medicinal (LQTM), Recife, PE, Brazil. Instituto de Ciências e Tecnologia em Neuroimunomodulação (INCT-NIM) Universidade Federal de Pernambuco, Departamento de Bioquímica, Recife, Pernambuco, Brasil. Universite de Nantes, Cibles et médicaments des infections et du cancer, IICiMed, EA 1155, Nantes, F-44000, France. Universidade Federal de Pernambuco, Departamento de Antibióticos, Recife, Pernambuco, Brasil. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. Electronic address: baratte@sb-roscoff.fr. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. |
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Garcia-Princival IMRPrincival JLDias da Silva Ede Arruda Lima SMCarregosa JCWisniewski A Jrde Lucena CCOHalwass FAlves Franca JAFerreira LFGRHernandes MZSaraiva KLAPeixoto CABaratte BRobert TBach SGomes DCGuedes Paiva PMMarchand PRodrigues MDDGonçalves da Silva T.2023-04-10T14:26:59Z2023-04-10T14:26:59Z2021GARCIA-PRINCIVAL, Iza Mirela Rodini; PRINCIVAL, Jefferson Luiz; SILVA, Emmanuel Dias da; LIMA, Sandrine Maria de Arruda; CARREGOSA, Jhonattas Carvalho; WISNIEWSKI JUNIOR, Alberto; LUCENA, Caio Cézar Oliveira de; HALWASS, Fernando; FRANCA, José Adonias Alves; FERREIRA, Luiz Felipe Gomes Rebello. Streptomyces hygroscopicus UFPEDA 3370: a valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells. Chemico-Biological Interactions, [S.L.], v. 333, p. 109316, jan. 2021. Elsevier BV. http://dx.doi.org/10.1016/j.cbi.2020.109316.1872-7786https://www.arca.fiocruz.br/handle/icict/5772410.1016/j.cbi.2020.109316porStreptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz, Instituto Aggeu Magalhães, Laboratório de Ultraestrutura, Recife, PE, Brazil. Universidade Federal de Pernambuco, Departamento de Antibióticos, Recife, Pernambuco, Brasil. Universidade Federal de Pernambuco, Departamento de Química Fundamental, Recife, Pernambuco, Brasil. Universidade Federal de Sergipe, Departamento de Química, Aracaju, Sergipe, Brasil. Universidade Federal de Pernambuco, Departamento de Ciências Farmacêuticas, Laboratório de Química Teórica Medicinal (LQTM), Recife, PE, Brazil. Instituto de Ciências e Tecnologia em Neuroimunomodulação (INCT-NIM) Universidade Federal de Pernambuco, Departamento de Bioquímica, Recife, Pernambuco, Brasil. Universite de Nantes, Cibles et médicaments des infections et du cancer, IICiMed, EA 1155, Nantes, F-44000, France. Universidade Federal de Pernambuco, Departamento de Antibióticos, Recife, Pernambuco, Brasil. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. Electronic address: baratte@sb-roscoff.fr. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France.Streptomyces hygroscopicus UFPEDA 3370 was fermented in submerged cultivation and the biomass extract was partitioned, obtaining a fraction purified named EB1. After purification of EB1 fraction, nigericin free acid was obtained and identified. Nigericin presented cytotoxic activity against several cancer cell lines, being most active against HL-60 (human leukemia) and HCT-116 (human colon carcinoma) cell lines, presenting IC50 and (IS) values: 0.0014 μM, (30.0) and 0.0138 μM (3.0), respectively. On HCT-116, nigericin caused apoptosis and autophagy. In this study, nigericin was also screened both in vitro and in silico against a panel of cancer-related kinases. Nigericin was able to inhibit both JAK3 and GSK-3β kinases in vitro and its binding affinities were mapped through the intermolecular interactions with each target in silico.AutophagyNatural productsNigericinProtein kinasesApoptosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALStreptomyces hygroscopicus UFPEDA 3370 A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells.pdfStreptomyces hygroscopicus UFPEDA 3370 A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells.pdfapplication/pdf8163250https://www.arca.fiocruz.br/bitstream/icict/57724/2/Streptomyces%20hygroscopicus%20UFPEDA%203370%20A%20valuable%20source%20of%20the%20potent%20cytotoxic%20agent%20nigericin%20and%20its%20evaluation%20against%20human%20colorectal%20cancer%20cells.pdff29e16d7c1d3d1a201f4e58e716d2c04MD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/57724/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/577242023-05-29 11:37:07.101oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-29T14:37:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Streptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells |
| title |
Streptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells |
| spellingShingle |
Streptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells Garcia-Princival IMR Autophagy Natural products Nigericin Protein kinases Apoptosis |
| title_short |
Streptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells |
| title_full |
Streptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells |
| title_fullStr |
Streptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells |
| title_full_unstemmed |
Streptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells |
| title_sort |
Streptomyces hygroscopicus UFPEDA 3370: A valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells |
| author |
Garcia-Princival IMR |
| author_facet |
Garcia-Princival IMR Princival JL Dias da Silva E de Arruda Lima SM Carregosa JC Wisniewski A Jr de Lucena CCO Halwass F Alves Franca JA Ferreira LFGR Hernandes MZ Saraiva KLA Peixoto CA Baratte B Robert T Bach S Gomes DC Guedes Paiva PM Marchand P Rodrigues MDD Gonçalves da Silva T. |
| author_role |
author |
| author2 |
Princival JL Dias da Silva E de Arruda Lima SM Carregosa JC Wisniewski A Jr de Lucena CCO Halwass F Alves Franca JA Ferreira LFGR Hernandes MZ Saraiva KLA Peixoto CA Baratte B Robert T Bach S Gomes DC Guedes Paiva PM Marchand P Rodrigues MDD Gonçalves da Silva T. |
| author2_role |
author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Garcia-Princival IMR Princival JL Dias da Silva E de Arruda Lima SM Carregosa JC Wisniewski A Jr de Lucena CCO Halwass F Alves Franca JA Ferreira LFGR Hernandes MZ Saraiva KLA Peixoto CA Baratte B Robert T Bach S Gomes DC Guedes Paiva PM Marchand P Rodrigues MDD Gonçalves da Silva T. |
| dc.subject.en.en_US.fl_str_mv |
Autophagy Natural products Nigericin Protein kinases Apoptosis |
| topic |
Autophagy Natural products Nigericin Protein kinases Apoptosis |
| description |
Fundação Oswaldo Cruz, Instituto Aggeu Magalhães, Laboratório de Ultraestrutura, Recife, PE, Brazil. Universidade Federal de Pernambuco, Departamento de Antibióticos, Recife, Pernambuco, Brasil. Universidade Federal de Pernambuco, Departamento de Química Fundamental, Recife, Pernambuco, Brasil. Universidade Federal de Sergipe, Departamento de Química, Aracaju, Sergipe, Brasil. Universidade Federal de Pernambuco, Departamento de Ciências Farmacêuticas, Laboratório de Química Teórica Medicinal (LQTM), Recife, PE, Brazil. Instituto de Ciências e Tecnologia em Neuroimunomodulação (INCT-NIM) Universidade Federal de Pernambuco, Departamento de Bioquímica, Recife, Pernambuco, Brasil. Universite de Nantes, Cibles et médicaments des infections et du cancer, IICiMed, EA 1155, Nantes, F-44000, France. Universidade Federal de Pernambuco, Departamento de Antibióticos, Recife, Pernambuco, Brasil. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. Electronic address: baratte@sb-roscoff.fr. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. Sorbonne Université, CNRS, UMR8227, Integrative Biology of Marine Models Laboratory (LBI2M), Station Biologique de Roscoff, F-29680, Roscoff, France; Sorbonne Université, CNRS, FR2424, Kinase Inhibitor Specialized Screening Facility - KISSf, Station Biologique, F-29688, Roscoff, France. |
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2021 |
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2021 |
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2023-04-10T14:26:59Z |
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GARCIA-PRINCIVAL, Iza Mirela Rodini; PRINCIVAL, Jefferson Luiz; SILVA, Emmanuel Dias da; LIMA, Sandrine Maria de Arruda; CARREGOSA, Jhonattas Carvalho; WISNIEWSKI JUNIOR, Alberto; LUCENA, Caio Cézar Oliveira de; HALWASS, Fernando; FRANCA, José Adonias Alves; FERREIRA, Luiz Felipe Gomes Rebello. Streptomyces hygroscopicus UFPEDA 3370: a valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells. Chemico-Biological Interactions, [S.L.], v. 333, p. 109316, jan. 2021. Elsevier BV. http://dx.doi.org/10.1016/j.cbi.2020.109316. |
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GARCIA-PRINCIVAL, Iza Mirela Rodini; PRINCIVAL, Jefferson Luiz; SILVA, Emmanuel Dias da; LIMA, Sandrine Maria de Arruda; CARREGOSA, Jhonattas Carvalho; WISNIEWSKI JUNIOR, Alberto; LUCENA, Caio Cézar Oliveira de; HALWASS, Fernando; FRANCA, José Adonias Alves; FERREIRA, Luiz Felipe Gomes Rebello. Streptomyces hygroscopicus UFPEDA 3370: a valuable source of the potent cytotoxic agent nigericin and its evaluation against human colorectal cancer cells. Chemico-Biological Interactions, [S.L.], v. 333, p. 109316, jan. 2021. Elsevier BV. http://dx.doi.org/10.1016/j.cbi.2020.109316. 1872-7786 10.1016/j.cbi.2020.109316 |
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