Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12552 |
Resumo: | Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil. |
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Morais, Lena Lilian Canto de SáGarza, Daniel RiosLoureiro, Edvaldo Carlos BritoVale, Elivam RodriguesSantos, Denise Suéllem Amorim de SousaCorrêa, Vanessa CavaleiroSousa, Nayara RufinoGurjão, Tereza Cristina MonteiroSantos, Elisabeth Conceição de OliveiraVieira, Verônica VianaFonseca, Erica Lourenço daVicente, Ana Carolina Paulo2016-01-19T11:54:28Z2016-01-19T11:54:28Z2013MORAIS, Lena Lillian Canto de Sá; et al. Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region. Plos One, v.8, n.11, e81372, 12p, 2013.10.1371/journal.pone.0081372https://www.arca.fiocruz.br/handle/icict/125521932-6203engPlos OneAmazôniaEstudos genéticosEstudos populacionaisVibrio CholoraeBrazilian Amazon aquatic ecosystemEnvironmental V. choleraeGenetic studyVibrio CholoraeCóleraPopulation and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Regioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção Bacteriologia. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ, Brasil.Vibrio cholerae is a natural inhabitant of many aquatic environments in the world. Biotypes harboring similar virulence-related gene clusters are the causative agents of epidemic cholera, but the majority of strains are harmless to humans. Since 1971, environmental surveillance for potentially pathogenic V. cholerae has resulted in the isolation of many strains from the Brazilian Amazon aquatic ecosystem. Most of these strains are from the non-O1/non-O139 serogroups (NAGs), but toxigenic O1 strains were isolated during the Latin America cholera epidemic in the region (1991-1996). A collection of environmental V. cholerae strains from the Brazilian Amazon belonging to pre-epidemic (1977-1990), epidemic (1991-1996), and post-epidemic (1996-2007) periods in the region, was analyzed. The presence of genes related to virulence within the species and the genetic relationship among the strains were studied. These variables and the information available concerning the strains were used to build a Bayesian multivariate dependency model to distinguish the importance of each variable in determining the others. Some genes related to the epidemic strains were found in environmental NAGs during and after the epidemic. Significant diversity among the virulence-related gene content was observed among O1 strains isolated from the environment during the epidemic period, but not from clinical isolates, which were analyzed as controls. Despite this diversity, these strains exhibited similar PFGE profiles. PFGE profiles were significant while separating potentially epidemic clones from indigenous strains. No significant correlation with isolation source, place or period was observed. The presence of the WASA-1 prophage significantly correlated with serogroups, PFGE profiles, and the presence of virulence-related genes. This study provides a broad characterization of the environmental V. cholerae population from the Amazon, and also highlights the importance of identifying precisely defined genetic markers such as the WASA-1 prophage for the surveillance of cholera.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12552/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALveronica_vieira_etal_IOC_2013.pdfveronica_vieira_etal_IOC_2013.pdfapplication/pdf5184549https://www.arca.fiocruz.br/bitstream/icict/12552/2/veronica_vieira_etal_IOC_2013.pdf0e7af8b39f34dca4e9aab09f387d4f2bMD52TEXTveronica_vieira_etal_IOC_2013.pdf.txtveronica_vieira_etal_IOC_2013.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region |
| title |
Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region |
| spellingShingle |
Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region Morais, Lena Lilian Canto de Sá Amazônia Estudos genéticos Estudos populacionais Vibrio Cholorae Brazilian Amazon aquatic ecosystem Environmental V. cholerae Genetic study Vibrio Cholorae Cólera |
| title_short |
Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region |
| title_full |
Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region |
| title_fullStr |
Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region |
| title_full_unstemmed |
Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region |
| title_sort |
Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region |
| author |
Morais, Lena Lilian Canto de Sá |
| author_facet |
Morais, Lena Lilian Canto de Sá Garza, Daniel Rios Loureiro, Edvaldo Carlos Brito Vale, Elivam Rodrigues Santos, Denise Suéllem Amorim de Sousa Corrêa, Vanessa Cavaleiro Sousa, Nayara Rufino Gurjão, Tereza Cristina Monteiro Santos, Elisabeth Conceição de Oliveira Vieira, Verônica Viana Fonseca, Erica Lourenço da Vicente, Ana Carolina Paulo |
| author_role |
author |
| author2 |
Garza, Daniel Rios Loureiro, Edvaldo Carlos Brito Vale, Elivam Rodrigues Santos, Denise Suéllem Amorim de Sousa Corrêa, Vanessa Cavaleiro Sousa, Nayara Rufino Gurjão, Tereza Cristina Monteiro Santos, Elisabeth Conceição de Oliveira Vieira, Verônica Viana Fonseca, Erica Lourenço da Vicente, Ana Carolina Paulo |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Morais, Lena Lilian Canto de Sá Garza, Daniel Rios Loureiro, Edvaldo Carlos Brito Vale, Elivam Rodrigues Santos, Denise Suéllem Amorim de Sousa Corrêa, Vanessa Cavaleiro Sousa, Nayara Rufino Gurjão, Tereza Cristina Monteiro Santos, Elisabeth Conceição de Oliveira Vieira, Verônica Viana Fonseca, Erica Lourenço da Vicente, Ana Carolina Paulo |
| dc.subject.other.pt_BR.fl_str_mv |
Amazônia Estudos genéticos Estudos populacionais |
| topic |
Amazônia Estudos genéticos Estudos populacionais Vibrio Cholorae Brazilian Amazon aquatic ecosystem Environmental V. cholerae Genetic study Vibrio Cholorae Cólera |
| dc.subject.en.pt_BR.fl_str_mv |
Vibrio Cholorae Brazilian Amazon aquatic ecosystem Environmental V. cholerae Genetic study |
| dc.subject.decs.pt_BR.fl_str_mv |
Vibrio Cholorae Cólera |
| description |
Instituto Evandro Chagas. Seção Ambiental. Ananindeua, PA, Brasil. |
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2013 |
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2013 |
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2016-01-19T11:54:28Z |
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2016-01-19T11:54:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MORAIS, Lena Lillian Canto de Sá; et al. Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region. Plos One, v.8, n.11, e81372, 12p, 2013. |
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https://www.arca.fiocruz.br/handle/icict/12552 |
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10.1371/journal.pone.0081372 |
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1932-6203 |
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MORAIS, Lena Lillian Canto de Sá; et al. Population and Genetic Study of Vibrio cholerae from the Amazon Environment Confirms that the WASA-1 Prophage Is the Main Marker of the Epidemic Strain that Circulated in the Region. Plos One, v.8, n.11, e81372, 12p, 2013. 10.1371/journal.pone.0081372 1932-6203 |
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