Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase

Detalhes bibliográficos
Autor(a) principal: Bourguignon, S. C.
Data de Publicação: 1998
Outros Autores: Meirelles, M. N., Pacheco, R. S., De Simone, Giovanni
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43928
Resumo: Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
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spelling Bourguignon, S. C.Meirelles, M. N.Pacheco, R. S.De Simone, Giovanni2020-10-12T12:36:01Z2020-10-12T12:36:01Z1998BOURGUIGNON, S. C. et al. Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n. 2, p. 219-224, Mar./Apr. 1998.0074-0276https://www.arca.fiocruz.br/handle/icict/4392810.1590/S0074-027619980002000171678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Trypanosoma cruziTriosephosphate isomerasePurificationSequenceTrypanosoma cruziTriosephosphate isomerasePurificationSequencePurification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomeraseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Sistemática Bioquímica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Microseqüenciamento de Proteínas. Rio de Janeiro, RJ, Brasil.The enzyme triosephosphate isomerase (TPI, EC 5.3.1.1) was purified from extracts of epimastigote forms of Trypanosoma cruzi. The purification steps included: hydrophobic interaction chromatography on phenyl-Sepharose, CM-Sepharose, and high performance liquid gel filtration chromatography. The CM-Sepharose material contained two bands (27 and 25 kDa) with similar isoelectric points (pI 9.3-9.5) which could be separated by gel filtration in high performance liquid chromatography. Polyclonal antibodies raised against the porcine TPI detected one single polypeptide on western blot with a molecular weight (27 kDa) identical to that purified from T. cruzi. These antibodies also recognized only one band of identical molecular weight in western blots of several other trypanosomatids (Blastocrithidia culicis, Crithidia desouzai, Phytomonas serpens, Herpertomonas samuelpessoai). The presence of only one enzymatic form of TPI in T. cruzi epimastigotes was confirmed by agarose gel activity assay and its localization was established by immunocytochemical analysis. The T. cruzi purified TPI (as well as other trypanosomatid' TPIs) is a dimeric protein, composed of two identical subunits with an approximate mw of 27,000 and it is resolved on two dimensional gel electrophoresis with a pI of 9.3. Sequence analysis of the N-terminal portion of the 27 kDa protein revealed a high homology to Leishmania mexicana and T. brucei proteins.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43928/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRSPacheco_SalvatoreDeSimone_etal_IOC_1998.pdfRSPacheco_SalvatoreDeSimone_etal_IOC_1998.pdfapplication/pdf418527https://www.arca.fiocruz.br/bitstream/icict/43928/2/RSPacheco_SalvatoreDeSimone_etal_IOC_1998.pdff563e553ec0cac53219592cf7c88da40MD52TEXTRSPacheco_SalvatoreDeSimone_etal_IOC_1998.pdf.txtRSPacheco_SalvatoreDeSimone_etal_IOC_1998.pdf.txtExtracted texttext/plain25707https://www.arca.fiocruz.br/bitstream/icict/43928/3/RSPacheco_SalvatoreDeSimone_etal_IOC_1998.pdf.txtbdcfc15dc0d8b956762175866be38a9aMD53icict/439282020-10-13 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dc.title.pt_BR.fl_str_mv Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase
title Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase
spellingShingle Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase
Bourguignon, S. C.
Trypanosoma cruzi
Triosephosphate isomerase
Purification
Sequence
Trypanosoma cruzi
Triosephosphate isomerase
Purification
Sequence
title_short Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase
title_full Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase
title_fullStr Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase
title_full_unstemmed Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase
title_sort Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase
author Bourguignon, S. C.
author_facet Bourguignon, S. C.
Meirelles, M. N.
Pacheco, R. S.
De Simone, Giovanni
author_role author
author2 Meirelles, M. N.
Pacheco, R. S.
De Simone, Giovanni
author2_role author
author
author
dc.contributor.author.fl_str_mv Bourguignon, S. C.
Meirelles, M. N.
Pacheco, R. S.
De Simone, Giovanni
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Triosephosphate isomerase
Purification
Sequence
topic Trypanosoma cruzi
Triosephosphate isomerase
Purification
Sequence
Trypanosoma cruzi
Triosephosphate isomerase
Purification
Sequence
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Triosephosphate isomerase
Purification
Sequence
description Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.
publishDate 1998
dc.date.issued.fl_str_mv 1998
dc.date.accessioned.fl_str_mv 2020-10-12T12:36:01Z
dc.date.available.fl_str_mv 2020-10-12T12:36:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BOURGUIGNON, S. C. et al. Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n. 2, p. 219-224, Mar./Apr. 1998.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43928
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761998000200017
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BOURGUIGNON, S. C. et al. Purification and Partial Characterization of Trypanosoma cruzi Triosephosphate Isomerase. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n. 2, p. 219-224, Mar./Apr. 1998.
0074-0276
10.1590/S0074-02761998000200017
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43928
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/43928/3/RSPacheco_SalvatoreDeSimone_etal_IOC_1998.pdf.txt
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