Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major

Detalhes bibliográficos
Autor(a) principal: Gomes, Flavia Lima Ribeiro
Data de Publicação: 2014
Outros Autores: Lima, Eric Henrique Roma de, Carneiro, Matheus Batista Heitor, Doria, Nicole A., Sacks, David L., Petersa, Nathan C. Petersa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10920
Resumo: NIH-CAPES
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spelling Gomes, Flavia Lima RibeiroLima, Eric Henrique Roma deCarneiro, Matheus Batista HeitorDoria, Nicole A.Sacks, David L.Petersa, Nathan C. Petersa2015-06-18T18:35:21Z2015-06-18T18:35:21Z2014GOMES, Flavia Lima Ribeiro et al. Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major. Infection and Immunity, v.82, n.7, p.2713–2727, 2014.0019-9567https://www.arca.fiocruz.br/handle/icict/1092010.1128/IAI.01600-131098-5522NIH-CAPESNational Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Bethesda, MD, USA.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Imunologia e Imunogenética em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil / National Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Bethesda, MD, USA / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Gnotobiologia e Imunologia. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.National Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Bethesda, MD, USA / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Gnotobiologia e Imunologia. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.National Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Bethesda, MD, USA.National Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Bethesda, MD, USA.National Institute of Allergy and Infectious Diseases. Laboratory of Parasitic Diseases. Bethesda, MD, USA.The route of pathogen inoculation by needle has been shown to influence the outcome of infection. Employing needle inoculation of the obligately intracellular parasite Leishmania major, which is transmitted in nature following intradermal (i.d.) deposition of parasites by the bite of an infected sand fly, we identified differences in the preexisting and acute cellular responses in mice following i.d. inoculation of the ear, subcutaneous (s.c.) inoculation of the footpad, or inoculation of the peritoneal cavity (intraperitoneal [i.p.] inoculation). Initiation of infection at different sites was associated with different phagocytic populations. Neutrophils were the dominant infected cells following i.d., but not s.c. or i.p., inoculation. Inoculation of the ear dermis resulted in higher frequencies of total and infected neutrophils than inoculation of the footpad, and these higher frequencies were associated with a 10-fold increase in early parasite loads. Following inoculation of the ear in the absence of neutrophils, parasite phagocytosis by other cell types did not increase, and fewer parasites were able to establish infection. The frequency of infected neutrophils within the total infected CD11b+ population was higher than the frequency of total neutrophils within the total CD11b+ population, demonstrating that neutrophils are overrepresented as a proportion of infected cells. Employing i.d. inoculation to model sand fly transmission of parasites has significant consequences for infection outcome relative to that of s.c. or i.p. inoculation, including the phenotype of infected cells and the number of parasites that establish infection. Vector-borne infections initiated in the dermis likely involve adaptations to this unique microenvironment. Bypassing or altering this initial step has significant consequences for infection.engAmerican Society for MicrobiologySite-dependent recruitment of inflammatory cells determines the effective dose of leishmania majorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLeishmaniaLeishmania majorNeutrophilsAntigens, CD11bLeishmaniaLeishmania majorNeutrófilosAntígenos CD11binfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/10920/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALSite-Dependent Recruitment of Inflammatory Cells Determines the.pdfSite-Dependent Recruitment of Inflammatory Cells Determines the.pdfapplication/pdf4547942https://www.arca.fiocruz.br/bitstream/icict/10920/2/Site-Dependent%20Recruitment%20of%20Inflammatory%20Cells%20Determines%20the.pdfa0c07295ddd9aca2f1ca951279b7bebbMD52TEXTSite-Dependent Recruitment of Inflammatory Cells Determines the.pdf.txtSite-Dependent Recruitment of Inflammatory Cells Determines the.pdf.txtExtracted texttext/plain65046https://www.arca.fiocruz.br/bitstream/icict/10920/3/Site-Dependent%20Recruitment%20of%20Inflammatory%20Cells%20Determines%20the.pdf.txt0c9d71f1ce1226ffc04008ba44f0922fMD53icict/109202019-09-16 11:21:15.797oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-09-16T14:21:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major
title Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major
spellingShingle Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major
Gomes, Flavia Lima Ribeiro
Leishmania
Leishmania major
Neutrophils
Antigens, CD11b
Leishmania
Leishmania major
Neutrófilos
Antígenos CD11b
title_short Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major
title_full Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major
title_fullStr Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major
title_full_unstemmed Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major
title_sort Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major
author Gomes, Flavia Lima Ribeiro
author_facet Gomes, Flavia Lima Ribeiro
Lima, Eric Henrique Roma de
Carneiro, Matheus Batista Heitor
Doria, Nicole A.
Sacks, David L.
Petersa, Nathan C. Petersa
author_role author
author2 Lima, Eric Henrique Roma de
Carneiro, Matheus Batista Heitor
Doria, Nicole A.
Sacks, David L.
Petersa, Nathan C. Petersa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Flavia Lima Ribeiro
Lima, Eric Henrique Roma de
Carneiro, Matheus Batista Heitor
Doria, Nicole A.
Sacks, David L.
Petersa, Nathan C. Petersa
dc.subject.en.pt_BR.fl_str_mv Leishmania
Leishmania major
Neutrophils
Antigens, CD11b
topic Leishmania
Leishmania major
Neutrophils
Antigens, CD11b
Leishmania
Leishmania major
Neutrófilos
Antígenos CD11b
dc.subject.decs.pt_BR.fl_str_mv Leishmania
Leishmania major
Neutrófilos
Antígenos CD11b
description NIH-CAPES
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-18T18:35:21Z
dc.date.available.fl_str_mv 2015-06-18T18:35:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOMES, Flavia Lima Ribeiro et al. Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major. Infection and Immunity, v.82, n.7, p.2713–2727, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10920
dc.identifier.issn.none.fl_str_mv 0019-9567
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.01600-13
dc.identifier.eissn.none.fl_str_mv 1098-5522
identifier_str_mv GOMES, Flavia Lima Ribeiro et al. Site-dependent recruitment of inflammatory cells determines the effective dose of leishmania major. Infection and Immunity, v.82, n.7, p.2713–2727, 2014.
0019-9567
10.1128/IAI.01600-13
1098-5522
url https://www.arca.fiocruz.br/handle/icict/10920
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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