Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/47962 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil. |
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Granato, Marcela Q.Mello, Thaís P.Nascimento, Renata S.Pereira, Marcos D.Rosa, Thabatta L. S. A.Pessolani, Maria C. V.McCann, MalachyDevereux, MichaelBranquinha, Marta H.Santos, André L. S.Kneipp, Lucimar F.2021-07-02T17:52:36Z2021-07-02T17:52:36Z2021GRANATO, Marcela Q. et al. Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae. Front. Microbiol., v. 12, Article 641258, 12 p, Apr. 2021.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/4796210.3389/fmicb.2021.641258engFrontiers MediaCromoblastomicoseInteração celularFungos dematiaceososAtividade antifúngicaFungos dematiaceosos1,10-phenanthroline-5,6-dioneChromoblastomycosisDematiaceous fungiAntifungal activityCellular interactionSilver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Estudos Avançados de Microorganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Laboratório de Citotoxidade e Genotoxcidade. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Química. Laboratório de Citotoxidade e Genotoxcidade. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Department of Chemistry. Maynooth University. National University of Ireland. Maynooth, Ireland.Center for Biomimetic and Therapeutic Research. Focas Research Institute. Technological University Dublin. Dublin, Ireland.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Estudos Avançados de Microorganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Estudos Avançados de Microorganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Química. Laboratório de Citotoxidade e Genotoxcidade. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil.Phialophora verrucosa is a dematiaceous fungus that causes mainly chromoblastomycosis, but also disseminated infections such as phaeohyphomycosis and mycetoma. These diseases are extremely hard to treat and often refractory to current antifungal therapies. In this work, we have evaluated the effect of 1,10-phenanthroline-5,6-dione (phendione) and its metal-based complexes, [Ag (phendione)2]ClO4 and [Cu(phendione)3](ClO4)2.4H2O, against P. verrucosa, focusing on (i) conidial viability when combined with amphotericin B (AmB); (ii) biofilm formation and disarticulation events; (iii) in vitro interaction with human macrophages; and (iv) in vivo infection of Galleria mellonella larvae. The combination of AmB with each of the test compounds promoted the additive inhibition of P. verrucosa growth, as judged by the checkerboard assay. During the biofilm formation process over polystyrene surface, sub-minimum inhibitory concentrations (MIC) of phendione and its silver(I) and copper(II) complexes were able to reduce biomass and extracellular matrix production. Moreover, a mature biofilm treated with high concentrations of the test compounds diminished biofilm viability in a concentration-dependent manner. Pre-treatment of conidial cells with the test compounds did not alter the percentage of infected THP-1 macrophages; however, [Ag(phendione)2]ClO4 caused a significant reduction in the number of intracellular fungal cells compared to the untreated system. In addition, the killing process was significantly enhanced by post-treatment of infected macrophages with the test compounds. P. verrucosa induced a typically cell density-dependent effect on G. mellonella larvae death after 7 days of infection. Interestingly, exposure to the silver(I) complex protected the larvae from P. verrucosa infection. Collectively, the results corroborate the promising therapeutic potential of phendione-based drugs against fungal infections, including those caused by P. verrucosa.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47962/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcelaGranato_LKneipp_etal_IOC_2021.pdfMarcelaGranato_LKneipp_etal_IOC_2021.pdfapplication/pdf2778876https://www.arca.fiocruz.br/bitstream/icict/47962/2/MarcelaGranato_LKneipp_etal_IOC_2021.pdf6a43c26a3eb7d70a61467d8c8a0936d2MD52TEXTMarcelaGranato_LKneipp_etal_IOC_2021.pdf.txtMarcelaGranato_LKneipp_etal_IOC_2021.pdf.txtExtracted texttext/plain63312https://www.arca.fiocruz.br/bitstream/icict/47962/3/MarcelaGranato_LKneipp_etal_IOC_2021.pdf.txt1f1a6541b3d30cb64e8beb5b4e152932MD53icict/479622021-07-03 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| dc.title.pt_BR.fl_str_mv |
Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae |
| title |
Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae |
| spellingShingle |
Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae Granato, Marcela Q. Cromoblastomicose Interação celular Fungos dematiaceosos Atividade antifúngica Fungos dematiaceosos 1,10-phenanthroline-5,6-dione Chromoblastomycosis Dematiaceous fungi Antifungal activity Cellular interaction |
| title_short |
Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae |
| title_full |
Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae |
| title_fullStr |
Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae |
| title_full_unstemmed |
Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae |
| title_sort |
Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae |
| author |
Granato, Marcela Q. |
| author_facet |
Granato, Marcela Q. Mello, Thaís P. Nascimento, Renata S. Pereira, Marcos D. Rosa, Thabatta L. S. A. Pessolani, Maria C. V. McCann, Malachy Devereux, Michael Branquinha, Marta H. Santos, André L. S. Kneipp, Lucimar F. |
| author_role |
author |
| author2 |
Mello, Thaís P. Nascimento, Renata S. Pereira, Marcos D. Rosa, Thabatta L. S. A. Pessolani, Maria C. V. McCann, Malachy Devereux, Michael Branquinha, Marta H. Santos, André L. S. Kneipp, Lucimar F. |
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author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Granato, Marcela Q. Mello, Thaís P. Nascimento, Renata S. Pereira, Marcos D. Rosa, Thabatta L. S. A. Pessolani, Maria C. V. McCann, Malachy Devereux, Michael Branquinha, Marta H. Santos, André L. S. Kneipp, Lucimar F. |
| dc.subject.other.pt_BR.fl_str_mv |
Cromoblastomicose Interação celular Fungos dematiaceosos Atividade antifúngica Fungos dematiaceosos |
| topic |
Cromoblastomicose Interação celular Fungos dematiaceosos Atividade antifúngica Fungos dematiaceosos 1,10-phenanthroline-5,6-dione Chromoblastomycosis Dematiaceous fungi Antifungal activity Cellular interaction |
| dc.subject.en.pt_BR.fl_str_mv |
1,10-phenanthroline-5,6-dione Chromoblastomycosis Dematiaceous fungi Antifungal activity Cellular interaction |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021-07-02T17:52:36Z |
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2021-07-02T17:52:36Z |
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2021 |
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GRANATO, Marcela Q. et al. Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae. Front. Microbiol., v. 12, Article 641258, 12 p, Apr. 2021. |
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https://www.arca.fiocruz.br/handle/icict/47962 |
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1664-302X |
| dc.identifier.eissn.none.fl_str_mv |
10.3389/fmicb.2021.641258 |
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GRANATO, Marcela Q. et al. Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione Against Phialophora verrucosa: A Focus on the Interaction With Human Macrophages and Galleria mellonella Larvae. Front. Microbiol., v. 12, Article 641258, 12 p, Apr. 2021. 1664-302X 10.3389/fmicb.2021.641258 |
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Frontiers Media |
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Frontiers Media |
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