Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30361 |
Resumo: | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brasil. |
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Mesquita, Emersom C.Hottz, Eugenio D.Amancio, Rodrigo T.Carneiro, Alan B.Palhinha, LohannaCoelho, Lara E.Grinsztejn, BeatrizZimmerman, Guy A.Rondina, Matthew T.Weyrich, Andrew S.Bozza, Patrícia T.Bozza, Fernando A.2018-12-03T15:25:04Z2018-12-03T15:25:04Z2018MESQUITA, Emersom C. et al. Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals. Scientific Reports, v. 8, n. 1, p. 1-10, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3036110.1038/s41598-018-33403-0engNature ResearchPersistent platelet activation and apoptosis in virologically suppressed HIV-infected individualsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil./ Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Bioquímica. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST/AIDS. Rio de Janeiro, RJ, Brasil.University of Utah. Molecular Medicine Program and Department of Internal Medicine. Salt Lake City, Utah, USA.University of Utah. Molecular Medicine Program and Department of Internal Medicine. Salt Lake City, Utah, USA.University of Utah. Molecular Medicine Program and Department of Internal Medicine. Salt Lake City, Utah, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brasil / Instituto D'OR de Pesquisa e Ensino. Rio de Janeiro, RJ, Brasil.Cardiovascular diseases and thrombotic events became major clinical problems in the combined antiretroviral therapy (cART) era. Although the precise mechanisms behind these clinical problems have not been fully elucidated, a persistent pro-inflammatory state plays a central role. As platelets play important roles on both, thrombus formation and inflammatory/immune response, we aimed at investigating platelet function in HIV-infected subjects virologically controlled through cART. We evaluate parameters of activation, mitochondrial function and activation of apoptosis pathways in platelets from 30 HIV-infected individuals under stable cART and 36 healthy volunteers. Despite viral control achieved through cART, HIV-infected individuals exhibited increased platelet activation as indicated by P-selectin expression and platelet spreading when adhered on fibrinogen-coated surfaces. Platelets from HIV-infected subjects also exhibited mitochondrial dysfunction and activation of apoptosis pathways. Finally, thrombin stimuli induced lower levels of P-selectin translocation and RANTES secretion, but not TXA2 synthesis, in platelets from HIV-infected individuals compared to control; and labeling of platelet alpha granules showed reduced granule content in platelets from HIV-infected individuals when compared to healthy subjects. In summary, platelets derived from HIV-infected individuals under stable cART exhibit a phenotype of increased activation, activation of the intrinsic pathway of apoptosis and undermined granule secretion in response to thrombin.HIVAntiretroviral therapyPlateletcARTinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/30361/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALPersistent platelet_Lara_Coelho_INI_Lapclin-AIDS_2018.pdfPersistent platelet_Lara_Coelho_INI_Lapclin-AIDS_2018.pdfapplication/pdf2298716https://www.arca.fiocruz.br/bitstream/icict/30361/2/Persistent%20platelet_Lara_Coelho_INI_Lapclin-AIDS_2018.pdfd0e3eb4a070aebd6db8d158c13a3f206MD52TEXTPersistent platelet_Lara_Coelho_INI_Lapclin-AIDS_2018.pdf.txtPersistent platelet_Lara_Coelho_INI_Lapclin-AIDS_2018.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals |
| title |
Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals |
| spellingShingle |
Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals Mesquita, Emersom C. HIV Antiretroviral therapy Platelet cART |
| title_short |
Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals |
| title_full |
Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals |
| title_fullStr |
Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals |
| title_full_unstemmed |
Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals |
| title_sort |
Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals |
| author |
Mesquita, Emersom C. |
| author_facet |
Mesquita, Emersom C. Hottz, Eugenio D. Amancio, Rodrigo T. Carneiro, Alan B. Palhinha, Lohanna Coelho, Lara E. Grinsztejn, Beatriz Zimmerman, Guy A. Rondina, Matthew T. Weyrich, Andrew S. Bozza, Patrícia T. Bozza, Fernando A. |
| author_role |
author |
| author2 |
Hottz, Eugenio D. Amancio, Rodrigo T. Carneiro, Alan B. Palhinha, Lohanna Coelho, Lara E. Grinsztejn, Beatriz Zimmerman, Guy A. Rondina, Matthew T. Weyrich, Andrew S. Bozza, Patrícia T. Bozza, Fernando A. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mesquita, Emersom C. Hottz, Eugenio D. Amancio, Rodrigo T. Carneiro, Alan B. Palhinha, Lohanna Coelho, Lara E. Grinsztejn, Beatriz Zimmerman, Guy A. Rondina, Matthew T. Weyrich, Andrew S. Bozza, Patrícia T. Bozza, Fernando A. |
| dc.subject.en.pt_BR.fl_str_mv |
HIV Antiretroviral therapy Platelet cART |
| topic |
HIV Antiretroviral therapy Platelet cART |
| description |
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Medicina Intensiva. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2018 |
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2018-12-03T15:25:04Z |
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2018-12-03T15:25:04Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MESQUITA, Emersom C. et al. Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals. Scientific Reports, v. 8, n. 1, p. 1-10, 2018. |
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https://www.arca.fiocruz.br/handle/icict/30361 |
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2045-2322 |
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10.1038/s41598-018-33403-0 |
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MESQUITA, Emersom C. et al. Persistent platelet activation and apoptosis in virologically suppressed HIV-infected individuals. Scientific Reports, v. 8, n. 1, p. 1-10, 2018. 2045-2322 10.1038/s41598-018-33403-0 |
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Nature Research |
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Nature Research |
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