Transcriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans

Detalhes bibliográficos
Autor(a) principal: Maretti-Mira, Ana Claudia
Data de Publicação: 2012
Outros Autores: Bittner, Jaime, Oliveira Neto, Manoel Paes, Liu, Minghsun, Kang, Dezhi, Li, Huiying, Pirmez, Claude, Craft, Noah
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18853
Resumo: Harbor-UCLA Medical Center. Los Angeles Biomedical Research Institute. Department of Medicine. Division of Molecular Medicine. California, CA, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
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spelling Maretti-Mira, Ana ClaudiaBittner, JaimeOliveira Neto, Manoel PaesLiu, MinghsunKang, DezhiLi, HuiyingPirmez, ClaudeCraft, Noah2017-05-16T11:26:43Z2017-05-16T11:26:43Z2012MARRETI-MIRA, Ana Claudia et al. Transcriptome Patterns from Primary Cutaneous Leishmania braziliensis Infections Associate with Eventual Development of Mucosal Disease in Humans. PLoS Neglected Tropical Diseases, v. 6, n. 9, p. 1-11, Sept. 2012.1935-2727https://www.arca.fiocruz.br/handle/icict/1885310.1371/journal.pntd.00018161935-2735engPublic Library of ScienceLeishmaniose cutâneaDoença das mucosasHumanosLeishmaniose mucosaMucosal LeishmaniasisMucosal DiseaseCutaneous Leishmania braziliensisHumansTranscriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHarbor-UCLA Medical Center. Los Angeles Biomedical Research Institute. Department of Medicine. Division of Molecular Medicine. California, CA, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Harbor-UCLA Medical Center. Los Angeles Biomedical Research Institute. Department of Medicine. Division of Molecular Medicine. California, CA, USA .Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Ambulatório de Leishmanioses. Rio de Janeiro, RJ, Brasil.VA Medical Center West Los Angeles. Infectious Diseases Section (111 F) and Research Service. Los Angeles, CA, USA. / University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA.University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA / UCLA-DOE Institute for Genomics and Proteomics. Crump Institute for Molecular Imaging. Department of Molecular and Medical Pharmacology. Los Angeles, CA, USA.University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA / UCLA-DOE Institute for Genomics and Proteomics. Crump Institute for Molecular Imaging. Department of Molecular and Medical Pharmacology. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Harbor-UCLA Medical Center. Los Angeles Biomedical Research Institute. Department of Medicine. Division of Molecular Medicine. California, CA, USA / University of California Los Angeles. David Geffen School of Medicine. Los Angeles, CA, USA.Introduction: Localized Cutaneous Leishmaniasis (LCL) and Mucosal Leishmaniasis (ML) are two extreme clinical forms of American Tegumentary Leishmaniasis that usually begin as solitary primary cutaneous lesions. Host and parasite factors that influence the progression of LCL to ML are not completely understood. In this manuscript, we compare the gene expression profiles of primary cutaneous lesions from patients who eventually developed ML to those that did not. Methods: Using RNA-seq, we analyzed both the human and Leishmania transcriptomes in primary cutaneous lesions. Results: Limited number of reads mapping to Leishmania transcripts were obtained. For human transcripts, compared to ML patients, lesions from LCL patients displayed a general multi-polarization of the adaptive immune response and showed upregulation of genes involved in chemoattraction of innate immune cells and in antigen presentation. We also identified a potential transcriptional signature in the primary lesions that may predict long-term disease outcome. Conclusions: We were able to simultaneously sequence both human and Leishmania mRNA transcripts in primary cutaneous leishmaniasis lesions. Our results suggest an intrinsic difference in the immune capacity of LCL and ML patients. The findings correlate the complete cure of L. braziliensis infection with a controlled inflammatory response and a balanced activation of innate and adaptive immunity.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18853/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALanaclaudia_mira_etal_IOC_2012.pdfanaclaudia_mira_etal_IOC_2012.pdfapplication/pdf807875https://www.arca.fiocruz.br/bitstream/icict/18853/2/anaclaudia_mira_etal_IOC_2012.pdfaeaba6612c0cf5e1ba9cad4e5650d3b0MD52TEXTanaclaudia_mira_etal_IOC_2012.pdf.txtanaclaudia_mira_etal_IOC_2012.pdf.txtExtracted texttext/plain54705https://www.arca.fiocruz.br/bitstream/icict/18853/3/anaclaudia_mira_etal_IOC_2012.pdf.txt9925de751a976de5fadab1134c40397fMD53icict/188532019-10-23 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dc.title.pt_BR.fl_str_mv Transcriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans
title Transcriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans
spellingShingle Transcriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans
Maretti-Mira, Ana Claudia
Leishmaniose cutânea
Doença das mucosas
Humanos
Leishmaniose mucosa
Mucosal Leishmaniasis
Mucosal Disease
Cutaneous Leishmania braziliensis
Humans
title_short Transcriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans
title_full Transcriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans
title_fullStr Transcriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans
title_full_unstemmed Transcriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans
title_sort Transcriptome patterns from primary cutaneous Leishmania braziliensis infections associate with eventual development of mucosal disease in humans
author Maretti-Mira, Ana Claudia
author_facet Maretti-Mira, Ana Claudia
Bittner, Jaime
Oliveira Neto, Manoel Paes
Liu, Minghsun
Kang, Dezhi
Li, Huiying
Pirmez, Claude
Craft, Noah
author_role author
author2 Bittner, Jaime
Oliveira Neto, Manoel Paes
Liu, Minghsun
Kang, Dezhi
Li, Huiying
Pirmez, Claude
Craft, Noah
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Maretti-Mira, Ana Claudia
Bittner, Jaime
Oliveira Neto, Manoel Paes
Liu, Minghsun
Kang, Dezhi
Li, Huiying
Pirmez, Claude
Craft, Noah
dc.subject.other.pt_BR.fl_str_mv Leishmaniose cutânea
Doença das mucosas
Humanos
Leishmaniose mucosa
topic Leishmaniose cutânea
Doença das mucosas
Humanos
Leishmaniose mucosa
Mucosal Leishmaniasis
Mucosal Disease
Cutaneous Leishmania braziliensis
Humans
dc.subject.en.pt_BR.fl_str_mv Mucosal Leishmaniasis
Mucosal Disease
Cutaneous Leishmania braziliensis
Humans
description Harbor-UCLA Medical Center. Los Angeles Biomedical Research Institute. Department of Medicine. Division of Molecular Medicine. California, CA, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-05-16T11:26:43Z
dc.date.available.fl_str_mv 2017-05-16T11:26:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARRETI-MIRA, Ana Claudia et al. Transcriptome Patterns from Primary Cutaneous Leishmania braziliensis Infections Associate with Eventual Development of Mucosal Disease in Humans. PLoS Neglected Tropical Diseases, v. 6, n. 9, p. 1-11, Sept. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18853
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001816
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv MARRETI-MIRA, Ana Claudia et al. Transcriptome Patterns from Primary Cutaneous Leishmania braziliensis Infections Associate with Eventual Development of Mucosal Disease in Humans. PLoS Neglected Tropical Diseases, v. 6, n. 9, p. 1-11, Sept. 2012.
1935-2727
10.1371/journal.pntd.0001816
1935-2735
url https://www.arca.fiocruz.br/handle/icict/18853
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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