Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7872 |
Resumo: | Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil |
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Fortunato, GustavoVidal, Daniel Torres AndionKlein, WilfriedAlberto Neto, AlbertoAngrizani, AndréVasconcelos, Juliana FragaKaneto, Carla MartinsSouza, Bruno Solano de FreitasSantos, Ricardo Ribeiro dosSoares, Milena Botelho PereiraMacambira, Simone Garcia2014-07-07T13:20:11Z2014-07-07T13:20:11Z2014FORTUNATO, G. et al. Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. Pulmonary Pharmacology & Therapeutics, v. 27, n. 2, p. 144-149, 2014.1522-9629https://www.arca.fiocruz.br/handle/icict/787210.1016/j.pupt.2013.04.003engElsevier LtdRecovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Feira de Santana. Feira de Santana, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, BrasilUniversidade de São Paulo. Departamento de Biologia. Faculdade de Filosofia, Ciências e Letras. Ribeirão Preto, SP, Brasil / Instituto Nacional de Ciência e Tecnologia em Fisiologia Comparada. UNESP. Rio Claro, SP, BrasilUniversidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil / Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilCentro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, BrasilCentro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, BrasilUniversidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, BrasilUniversidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biofunção. Salvador, BA, BrasilEmphysema is a chronic obstructive pulmonary disease characterized abnormal dilatation of alveolar spaces, which impairs alveolar gas exchange, compromising the physical capacity of a patient due to airflow limitations. Here we tested the effects of G-CSF administration in pulmonary tissue and exercise capacity in emphysematous mice. C57Bl/6 female mice were treated with elastase intratracheally to induce emphysema. Their exercise capacities were evaluated in a treadmill. Lung histological sections were prepared to evaluate mean linear intercept measurement. Emphysematous mice were treated with G-CSF (3 cycles of 200 mg/kg/day for 5 consecutive days, with 7-day intervals) or saline and submitted to a third evaluation 8 weeks after treatment. Values of run distance and linear intercept measurement were expressed as mean SD and compared applying a paired t-test. Effects of treatment on these parameters were analyzed applying a Repeated Measures ANOVA, followed by Tukey’s post hoc analysis. p < 0.05 was considered statistically significant. Twenty eight days later, animals ran significantly less in a treadmill compared to normal mice (549.7 181.2 m and 821.7 131.3 m, respectively; p < 0.01). Treatment with G-CSF significantly increased the exercise capacity of emphysematous mice (719.6 200.5 m), whereas saline treatment had no effect on distance run (595.8 178.5 m). The PCR cytokines genes analysis did not detect difference between experimental groups. Morphometric analyses in the lung showed that saline-treated mice had a mean linear intercept significantly higher (p < 0.01) when compared to mice treated with G-CSF, which did not significantly differ from that of normal mice. Treatment with G-CSF promoted the recovery of exercise capacity and regeneration of alveolar structural alterations in emphysematous mice.Pulmonary emphysemaG-CSFMean linear interceptExercise capacityinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFortunato G Recovery of pulmonary....pdfFortunato G Recovery of pulmonary....pdfapplication/pdf1248393https://www.arca.fiocruz.br/bitstream/icict/7872/1/Fortunato%20G%20Recovery%20of%20pulmonary....pdff7fd45af2191e8451c0708517138c108MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7872/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTFortunato G Recovery of pulmonary....pdf.txtFortunato G Recovery of pulmonary....pdf.txtExtracted texttext/plain31940https://www.arca.fiocruz.br/bitstream/icict/7872/3/Fortunato%20G%20Recovery%20of%20pulmonary....pdf.txtd4d6d18c87002901ba41653d26907f1dMD53icict/78722023-03-15 14:33:15.383oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. |
| title |
Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. |
| spellingShingle |
Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. Fortunato, Gustavo Pulmonary emphysema G-CSF Mean linear intercept Exercise capacity |
| title_short |
Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. |
| title_full |
Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. |
| title_fullStr |
Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. |
| title_full_unstemmed |
Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. |
| title_sort |
Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. |
| author |
Fortunato, Gustavo |
| author_facet |
Fortunato, Gustavo Vidal, Daniel Torres Andion Klein, Wilfried Alberto Neto, Alberto Angrizani, André Vasconcelos, Juliana Fraga Kaneto, Carla Martins Souza, Bruno Solano de Freitas Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira Macambira, Simone Garcia |
| author_role |
author |
| author2 |
Vidal, Daniel Torres Andion Klein, Wilfried Alberto Neto, Alberto Angrizani, André Vasconcelos, Juliana Fraga Kaneto, Carla Martins Souza, Bruno Solano de Freitas Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira Macambira, Simone Garcia |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fortunato, Gustavo Vidal, Daniel Torres Andion Klein, Wilfried Alberto Neto, Alberto Angrizani, André Vasconcelos, Juliana Fraga Kaneto, Carla Martins Souza, Bruno Solano de Freitas Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira Macambira, Simone Garcia |
| dc.subject.en.pt_BR.fl_str_mv |
Pulmonary emphysema G-CSF Mean linear intercept Exercise capacity |
| topic |
Pulmonary emphysema G-CSF Mean linear intercept Exercise capacity |
| description |
Universidade Estadual de Feira de Santana. Feira de Santana, BA, Brasil |
| publishDate |
2014 |
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2014-07-07T13:20:11Z |
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2014-07-07T13:20:11Z |
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2014 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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publishedVersion |
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FORTUNATO, G. et al. Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. Pulmonary Pharmacology & Therapeutics, v. 27, n. 2, p. 144-149, 2014. |
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https://www.arca.fiocruz.br/handle/icict/7872 |
| dc.identifier.issn.none.fl_str_mv |
1522-9629 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.pupt.2013.04.003 |
| identifier_str_mv |
FORTUNATO, G. et al. Recovery of pulmonary structure and exercise capacity by treatment with granulocyte-colony stimulating factor (G-CSF) in a mouse model of emphysema. Pulmonary Pharmacology & Therapeutics, v. 27, n. 2, p. 144-149, 2014. 1522-9629 10.1016/j.pupt.2013.04.003 |
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Elsevier Ltd |
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Elsevier Ltd |
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