Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Souza, Tainã Luís de
Data de Publicação: 2019
Outros Autores: Silva, Aurea Virginia Andrade da, Pereira, Luiza de Oliveira Ramos, Figueiredo, Fabiano Borges, Mendes Junior, Artur Augusto Velho, Menezes, Rodrigo Caldas, Mendes-da-Cruz, Daniella A., Boité, Mariana Côrtes, Cupolillo, Elisa, Porrozzi, Renato, Morgado, Fernanda Nazaré
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36107
Resumo: 2020-04-02
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spelling Souza, Tainã Luís deSilva, Aurea Virginia Andrade daPereira, Luiza de Oliveira RamosFigueiredo, Fabiano BorgesMendes Junior, Artur Augusto VelhoMenezes, Rodrigo CaldasMendes-da-Cruz, Daniella A.Boité, Mariana CôrtesCupolillo, ElisaPorrozzi, RenatoMorgado, Fernanda Nazaré2019-10-03T12:02:58Z2019-10-03T12:02:58Z2019SOUZA, Tainã Luís de et al. Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis. Nature, v. 9, p. 1-14, 2019.0028-0836https://www.arca.fiocruz.br/handle/icict/3610710.1038/s41598-019-49344-11476-4687eng0028-0836Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-04-02Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Celular. Paraná, PR, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas Sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose Rio de Janeiro. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose Rio de Janeiro. Rio de Janeiro, RJ, Brasil.In canine visceral leishmaniasis (CVL), splenic white pulp (SWP) disorganization has been associated with disease progression, reduced cytokine and chemokine expression and failure to control the parasite load. This profile is compatible with the cellular exhaustion previously shown in human visceral leishmaniasis. The present study aimed to evaluate the in situ expression of cellular exhaustion markers and their relation to clinical signs, SWP disorganization and parasite load. Forty dogs naturally infected by Leishmania infantum were grouped according to levels of SWP organization and parasite load. SWP disorganization was associated with reductions in the periarteriolar lymphatic sheath and lymphoid follicles/mm2 and worsening of the disease. Apoptotic cells expressing CTLA-4+ increased in dogs with disorganized SWP and a high parasite load. In the same group, PD-L1 and LAG-3 gene expression were reduced. A higher number of CD21+TIM-3+ B cells was detected in disorganized spleens than in organized spleens. Apoptosis is involved in periarteriolar lymphatic sheath reduction and lymphoid follicle atrophy and is associated with CTLA-4+ cell reductions in the splenic tissue of dogs with visceral leishmaniasis (VL). Failure to control the parasite load was observed, suggesting that cell exhaustion followed by T and B cell apoptosis plays a role in the immunosuppression observed in CVL.Canine visceral leishmaniasisLeishmania infantumDogsSplenic white pulpinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/36107/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Souza_Tainã_etal_INI_2019.pdfve_Souza_Tainã_etal_INI_2019.pdfapplication/pdf8741281https://www.arca.fiocruz.br/bitstream/icict/36107/2/ve_Souza_Tain%c3%a3_etal_INI_2019.pdfeae9bb248479a1454b6bb5341b535ac4MD52TEXTve_Souza_Tainã_etal_INI_2019.pdf.txtve_Souza_Tainã_etal_INI_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis
title Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis
spellingShingle Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis
Souza, Tainã Luís de
Canine visceral leishmaniasis
Leishmania infantum
Dogs
Splenic white pulp
title_short Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis
title_full Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis
title_fullStr Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis
title_full_unstemmed Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis
title_sort Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis
author Souza, Tainã Luís de
author_facet Souza, Tainã Luís de
Silva, Aurea Virginia Andrade da
Pereira, Luiza de Oliveira Ramos
Figueiredo, Fabiano Borges
Mendes Junior, Artur Augusto Velho
Menezes, Rodrigo Caldas
Mendes-da-Cruz, Daniella A.
Boité, Mariana Côrtes
Cupolillo, Elisa
Porrozzi, Renato
Morgado, Fernanda Nazaré
author_role author
author2 Silva, Aurea Virginia Andrade da
Pereira, Luiza de Oliveira Ramos
Figueiredo, Fabiano Borges
Mendes Junior, Artur Augusto Velho
Menezes, Rodrigo Caldas
Mendes-da-Cruz, Daniella A.
Boité, Mariana Côrtes
Cupolillo, Elisa
Porrozzi, Renato
Morgado, Fernanda Nazaré
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Tainã Luís de
Silva, Aurea Virginia Andrade da
Pereira, Luiza de Oliveira Ramos
Figueiredo, Fabiano Borges
Mendes Junior, Artur Augusto Velho
Menezes, Rodrigo Caldas
Mendes-da-Cruz, Daniella A.
Boité, Mariana Côrtes
Cupolillo, Elisa
Porrozzi, Renato
Morgado, Fernanda Nazaré
dc.subject.en.pt_BR.fl_str_mv Canine visceral leishmaniasis
Leishmania infantum
Dogs
Splenic white pulp
topic Canine visceral leishmaniasis
Leishmania infantum
Dogs
Splenic white pulp
description 2020-04-02
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-10-03T12:02:58Z
dc.date.available.fl_str_mv 2019-10-03T12:02:58Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Tainã Luís de et al. Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis. Nature, v. 9, p. 1-14, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36107
dc.identifier.issn.pt_BR.fl_str_mv 0028-0836
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-019-49344-1
dc.identifier.eissn.none.fl_str_mv 1476-4687
identifier_str_mv SOUZA, Tainã Luís de et al. Pro-cellular exhaustion markers are associated with splenic microarchitecture disorganization and parasite load in dogs with visceral leishmaniasis. Nature, v. 9, p. 1-14, 2019.
0028-0836
10.1038/s41598-019-49344-1
1476-4687
url https://www.arca.fiocruz.br/handle/icict/36107
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv 0028-0836
publisher.none.fl_str_mv 0028-0836
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/36107/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/36107/2/ve_Souza_Tain%c3%a3_etal_INI_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/36107/3/ve_Souza_Tain%c3%a3_etal_INI_2019.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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