Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44273 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. |
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Suzukawa, Andréia AkemiZanluca, CamilaJorge, Natasha Andressa NogueiraNoronha, Lucia deKoishi, Andrea CristinePaula, Caroline B. V. deRebutini, Patrícia Z.Nagashima, SeigoHansel-Frose, Aruana F. F.Parreira, Vinícius S. C.Bordignon, JulianoMacDonald, Margaret R.Rice, Charles M.Passetti, FabioSantos, Claudia Nunes Duarte dos2020-10-30T16:19:11Z2020-10-30T16:19:11Z2020SUZUKAWA, Andréia A. et al. Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil. Journal of Infection, p. 1-10, 2020.0163-4453https://www.arca.fiocruz.br/handle/icict/4427310.1016/j.jinf.2020.09.028porBritish Infection Society; ElsevierDownregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Bioinformatics Group. Department of Computer Science and Interdisciplinary. Center for Bioinformatics. Leipzig, Germany.Pontifícia Universidade Católica do Paraná. Laboratório de Patologia Experimental, Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Laboratório de Patologia Experimental, Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Laboratório de Patologia Experimental, Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Laboratório de Patologia Experimental, Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Laboratory of Virology and Infectious Disease. The Rockefeller University. New York, USA.Laboratory of Virology and Infectious Disease. The Rockefeller University. New York, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Screening for genes differentially expressed in placental tissues, aiming to identify transcriptional signatures that may be involved in ZIKV congenital pathogenesis. Methods: Transcriptome data from placental tissues of pregnant women naturally infected with Zika virus during the third trimester were compared to those from women who tested negative for Zika infection. The findings were validated using both a cell culture model and an immunohistochemistry/morphological analysis of naturally infected placental tissues. Results: Transcriptome analysis revealed that Zika virus infection induces downregulation of insulin-like growth factor II (IGF2) gene, an essential factor for fetal development. The Caco-2 cell culture model that constitutively expresses IGF2 was used for the transcriptome validation. Asiatic and African Zika virus strains infection caused downregulated IGF2 gene expression in Caco-2 cells, whereas other flaviviruses, such as dengue serotype 1, West Nile and wild-type yellow fever viruses, had no effect on this gene expression. Immunohistochemical assays on decidual tissues corroborated our transcriptome analysis, showing that IGF2 is reduced in the decidua of Zika virus-infected women. Conclusions: Our results draw attention to IGF2 modulation in uterine tissues, and this finding is expected to support future studies on strategies to ameliorate the harmful effects of Zika virus infection during pregnancy.Zika VirusTranscriptomeInsulin-Like Growth Factor IIDeciduaVirus ZikaTranscriptomaFactor II del Crecimiento Similar a la InsulinaDeciduaFacteur de croissance IGF-IICaduquesZika virusPlacentaTranscriptomaFator de Crescimento Insulin-Like IIDecíduainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil |
| title |
Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil |
| spellingShingle |
Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil Suzukawa, Andréia Akemi Zika Virus Transcriptome Insulin-Like Growth Factor II Decidua Virus Zika Transcriptoma Factor II del Crecimiento Similar a la Insulina Decidua Facteur de croissance IGF-II Caduques Zika virus Placenta Transcriptoma Fator de Crescimento Insulin-Like II Decídua |
| title_short |
Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil |
| title_full |
Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil |
| title_fullStr |
Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil |
| title_full_unstemmed |
Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil |
| title_sort |
Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil |
| author |
Suzukawa, Andréia Akemi |
| author_facet |
Suzukawa, Andréia Akemi Zanluca, Camila Jorge, Natasha Andressa Nogueira Noronha, Lucia de Koishi, Andrea Cristine Paula, Caroline B. V. de Rebutini, Patrícia Z. Nagashima, Seigo Hansel-Frose, Aruana F. F. Parreira, Vinícius S. C. Bordignon, Juliano MacDonald, Margaret R. Rice, Charles M. Passetti, Fabio Santos, Claudia Nunes Duarte dos |
| author_role |
author |
| author2 |
Zanluca, Camila Jorge, Natasha Andressa Nogueira Noronha, Lucia de Koishi, Andrea Cristine Paula, Caroline B. V. de Rebutini, Patrícia Z. Nagashima, Seigo Hansel-Frose, Aruana F. F. Parreira, Vinícius S. C. Bordignon, Juliano MacDonald, Margaret R. Rice, Charles M. Passetti, Fabio Santos, Claudia Nunes Duarte dos |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Suzukawa, Andréia Akemi Zanluca, Camila Jorge, Natasha Andressa Nogueira Noronha, Lucia de Koishi, Andrea Cristine Paula, Caroline B. V. de Rebutini, Patrícia Z. Nagashima, Seigo Hansel-Frose, Aruana F. F. Parreira, Vinícius S. C. Bordignon, Juliano MacDonald, Margaret R. Rice, Charles M. Passetti, Fabio Santos, Claudia Nunes Duarte dos |
| dc.subject.en.pt_BR.fl_str_mv |
Zika Virus Transcriptome Insulin-Like Growth Factor II Decidua |
| topic |
Zika Virus Transcriptome Insulin-Like Growth Factor II Decidua Virus Zika Transcriptoma Factor II del Crecimiento Similar a la Insulina Decidua Facteur de croissance IGF-II Caduques Zika virus Placenta Transcriptoma Fator de Crescimento Insulin-Like II Decídua |
| dc.subject.es.pt_BR.fl_str_mv |
Virus Zika Transcriptoma Factor II del Crecimiento Similar a la Insulina Decidua |
| dc.subject.fr.pt_BR.fl_str_mv |
Facteur de croissance IGF-II Caduques |
| dc.subject.decs.pt_BR.fl_str_mv |
Zika virus Placenta Transcriptoma Fator de Crescimento Insulin-Like II Decídua |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. |
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2020 |
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2020-10-30T16:19:11Z |
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2020-10-30T16:19:11Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SUZUKAWA, Andréia A. et al. Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil. Journal of Infection, p. 1-10, 2020. |
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https://www.arca.fiocruz.br/handle/icict/44273 |
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0163-4453 |
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10.1016/j.jinf.2020.09.028 |
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SUZUKAWA, Andréia A. et al. Downregulation of IGF 2 expression in third trimester placental tissues from Zika virus infected women in Brazil. Journal of Infection, p. 1-10, 2020. 0163-4453 10.1016/j.jinf.2020.09.028 |
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British Infection Society; Elsevier |
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British Infection Society; Elsevier |
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