The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12623 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. |
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Freitas, Rafael Maciel deSylvestre, GabrielGandini, MarianaKoella, Jacob C.2016-01-26T15:04:13Z2016-01-26T15:04:13Z2013FREITAS, Rafael Maciel de; et al. The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed. Plos One, v.8, n.6, e65252, 5p, 2013.1932-6203https://www.arca.fiocruz.br/handle/icict/1262310.1371/journal.pone.0065252engPublic Library of ScienceThe Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feedinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Université de Neuchätel. Institut de Biologie. Switzerland.Background: Dengue virus (DENV) is transmitted by Aedes aegypti, a species that lives in close association with human dwellings. The behavior of DENV-infected mosquitoes needs further investigation, especially regarding the potential influence of DENV on mosquito biting motivation and avidity. Methodology/Principal findings: We orally challenged 4–5 day-old Ae. aegypti females with a low passage DENV serotype - 2 (DENV-2) to test whether the virus influences motivation to feed (the likelihood that a mosquito obtains a blood-meal and the size of its blood meal) and avidity (the likelihood to re-feed after an interrupted first blood-meal). To assay motivation, we offered mosquitoes an anesthetized mouse for 2, 3, 4 or 5 minutes 7 or 14 days after the initial blood meals and measured the time they started feeding. 60.5% of the unexposed mosquitoes fed on the mouse, but only 40.5% of the positive ones did. Exposed but negative mosquitoes behaved similarly to unexposed ones (55.0% feeding). Thus DENV-2 infection decreased the mosquitoes’ motivation to feed. To assay avidity, we offered the same mosquitoes a mouse two hours after the first round of feeding, and we measured the time at which they started probing. The exposed (positive or negative) mosquitoes were more likely to re-feed than the unexposed ones and, in particular, the size of the previous bloodmeal that kept mosquitoes from re-feeding was larger in the exposed than in the unexposed mosquitoes. Thus, DENV-2 infection increased mosquito avidity. Conclusions/Significance: DENV-2 significantly decreased the mosquitoes’ motivation to feed, but increased their avidity (even after taking account the amount of blood previously imbibed). As these are important components of transmission, we expect that the changes of the blood-feeding behaviour impact the vectorial capacity Ae. aegypti for dengue.DengueVirus Serotype-2Aedes aegyptiAedesDengueVírus da Dengueinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12623/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmariana2_gandini_etal_IOC_2013.pdfmariana2_gandini_etal_IOC_2013.pdfapplication/pdf364173https://www.arca.fiocruz.br/bitstream/icict/12623/2/mariana2_gandini_etal_IOC_2013.pdfea2cb4379c6859d6547f6b4257669d30MD52TEXTmariana2_gandini_etal_IOC_2013.pdf.txtmariana2_gandini_etal_IOC_2013.pdf.txtExtracted texttext/plain30285https://www.arca.fiocruz.br/bitstream/icict/12623/3/mariana2_gandini_etal_IOC_2013.pdf.txtb4e002f0e6bb11273fa63d70f97cd196MD53icict/126232023-09-05 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dc.title.pt_BR.fl_str_mv |
The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed |
title |
The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed |
spellingShingle |
The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed Freitas, Rafael Maciel de Dengue Virus Serotype-2 Aedes aegypti Aedes Dengue Vírus da Dengue |
title_short |
The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed |
title_full |
The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed |
title_fullStr |
The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed |
title_full_unstemmed |
The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed |
title_sort |
The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed |
author |
Freitas, Rafael Maciel de |
author_facet |
Freitas, Rafael Maciel de Sylvestre, Gabriel Gandini, Mariana Koella, Jacob C. |
author_role |
author |
author2 |
Sylvestre, Gabriel Gandini, Mariana Koella, Jacob C. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Freitas, Rafael Maciel de Sylvestre, Gabriel Gandini, Mariana Koella, Jacob C. |
dc.subject.en.pt_BR.fl_str_mv |
Dengue Virus Serotype-2 Aedes aegypti |
topic |
Dengue Virus Serotype-2 Aedes aegypti Aedes Dengue Vírus da Dengue |
dc.subject.decs.pt_BR.fl_str_mv |
Aedes Dengue Vírus da Dengue |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2016-01-26T15:04:13Z |
dc.date.available.fl_str_mv |
2016-01-26T15:04:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FREITAS, Rafael Maciel de; et al. The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed. Plos One, v.8, n.6, e65252, 5p, 2013. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/12623 |
dc.identifier.issn.none.fl_str_mv |
1932-6203 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0065252 |
identifier_str_mv |
FREITAS, Rafael Maciel de; et al. The Influence of Dengue Virus Serotype-2 Infection on Aedes aegypti (Diptera: Culicidae) Motivation and Avidity to Blood Feed. Plos One, v.8, n.6, e65252, 5p, 2013. 1932-6203 10.1371/journal.pone.0065252 |
url |
https://www.arca.fiocruz.br/handle/icict/12623 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Public Library of Science |
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Public Library of Science |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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