Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59576 |
Resumo: | Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University. Niterói, RJ, Brazil / Departments of Medicine. Division of Infectious Diseases and Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA. |
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Guimaraes, Allan J.Cerqueira, Mariana Duarte deZamith-Miranda, DanielLopez, Pablo H.Rodrigues, Marcio L.Pontes, BrunoViana, Nathan B.Deleon-Rodriguez, Carlos M.Rossi, Diego Conrado PereiraCasadevall, ArturoGomes, Andre M. O.Martinez, Luis R.Schnaar, Ronald L.Nosanchuk, Joshua D.Nimrichter, Leonardo2023-07-13T22:01:41Z2023-07-13T22:01:41Z2019GUIMARAES, Allan J. et al. Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages. Cellular Microbiology, v. 21, n. 3, p. 1-18, e12976, Sept. 2019.1462-5814https://www.arca.fiocruz.br/handle/icict/5957610.1111/CMI.129761462-5822engWileyAnimalsCell AdhesionCell LineEndocytosisHistoplasmaHost-Pathogen InteractionsMacrophagesMembrane MicrodomainsMice Inbred C57BLMice KnockoutGlycosphingolipidsHistoplasma capsulatumLipid raftsMacrophagesHost membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophagesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDepartment of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University. Niterói, RJ, Brazil / Departments of Medicine. Division of Infectious Diseases and Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA.Department of General Microbiology. Microbiology Institute. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Department of General Microbiology. Microbiology Institute. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Department of Pharmacology and Molecular Sciences. Johns Hopkins University School of Medicine. Baltimore, USA.Department of General Microbiology. Microbiology Institute. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.LPO-COPEA. Institute of Biomedical Sciences. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.LPO-COPEA. Institute of Biomedical Sciences. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil / LPO-COPEA. Institute of Physics. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, USA.Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, USA.Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, USA.Program of Structural Biology. Institute of Medical Biochemistry Leopoldo de Meis and National Institute of Science and Technology of Structural Biology and Bioimaging. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Biological Sciences. The University of Texas at El Paso. El Paso, USA.Department of Pharmacology and Molecular Sciences. Johns Hopkins University School of Medicine. Baltimore, USA.Departments of Medicine. Division of Infectious Diseases and Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA.Department of General Microbiology. Microbiology Institute. Federal University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Recognition and internalisation of intracellular pathogens by host cells is a multifactorial process, involving both stable and transient interactions. The plasticity of the host cell plasma membrane is fundamental in this infectious process. Here, the participation of macrophage lipid microdomains during adhesion and internalisation of the fungal pathogen Histoplasma capsulatum (Hc) was investigated. An increase in membrane lateral organisation, which is a characteristic of lipid microdomains, was observed during the first steps of Hc-macrophage interaction. Cholesterol enrichment in macrophage membranes around Hc contact regions and reduced levels of Hc-macrophage association after cholesterol removal also suggested the participation of lipid microdomains during Hc-macrophage interaction. Using optical tweezers to study cell-to-cell interactions, we showed that cholesterol depletion increased the time required for Hc adhesion. Additionally, fungal internalisation was significantly reduced under these conditions. Moreover, macrophages treated with the ceramide-glucosyltransferase inhibitor (P4r) and macrophages with altered ganglioside synthesis (from B4galnt1<sup>-/-</sup> mice) showed a deficient ability to interact with Hc. Coincubation of oligo-GM1 and treatment with Cholera toxin Subunit B, which recognises the ganglioside GM1, also reduced Hc association. Although purified GM1 did not alter Hc binding, treatment with P4 significantly increased the time required for Hc binding to macrophages. The content of CD18 was displaced from lipid microdomains in B4galnt1<sup>-/-</sup> macrophages. In addition, macrophages with reduced CD18 expression (CD18<sup>low</sup> ) were associated with Hc at levels similar to wild-type cells. Finally, CD11b and CD18 colocalised with GM1 during Hc-macrophage interaction. Our results indicate that lipid rafts and particularly complex gangliosides that reside in lipid rafts stabilise Hc-macrophage adhesion and mediate efficient internalisation during histoplasmosis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCellular Microbiology - 2018 - Guimar es - Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma.pdfCellular Microbiology - 2018 - Guimar es - Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma.pdfapplication/pdf1663656https://www.arca.fiocruz.br/bitstream/icict/59576/2/Cellular%20Microbiology%20-%202018%20-%20Guimar%20es%20-%20Host%20membrane%20glycosphingolipids%20and%20lipid%20microdomains%20facilitate%20Histoplasma.pdf8c98f7734a5fa248a65c0a4cc6b1d2aaMD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59576/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/595762023-07-13 20:59:12.061oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-13T23:59:12Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages |
| title |
Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages |
| spellingShingle |
Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages Guimaraes, Allan J. Animals Cell Adhesion Cell Line Endocytosis Histoplasma Host-Pathogen Interactions Macrophages Membrane Microdomains Mice Inbred C57BL Mice Knockout Glycosphingolipids Histoplasma capsulatum Lipid rafts Macrophages |
| title_short |
Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages |
| title_full |
Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages |
| title_fullStr |
Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages |
| title_full_unstemmed |
Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages |
| title_sort |
Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages |
| author |
Guimaraes, Allan J. |
| author_facet |
Guimaraes, Allan J. Cerqueira, Mariana Duarte de Zamith-Miranda, Daniel Lopez, Pablo H. Rodrigues, Marcio L. Pontes, Bruno Viana, Nathan B. Deleon-Rodriguez, Carlos M. Rossi, Diego Conrado Pereira Casadevall, Arturo Gomes, Andre M. O. Martinez, Luis R. Schnaar, Ronald L. Nosanchuk, Joshua D. Nimrichter, Leonardo |
| author_role |
author |
| author2 |
Cerqueira, Mariana Duarte de Zamith-Miranda, Daniel Lopez, Pablo H. Rodrigues, Marcio L. Pontes, Bruno Viana, Nathan B. Deleon-Rodriguez, Carlos M. Rossi, Diego Conrado Pereira Casadevall, Arturo Gomes, Andre M. O. Martinez, Luis R. Schnaar, Ronald L. Nosanchuk, Joshua D. Nimrichter, Leonardo |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Guimaraes, Allan J. Cerqueira, Mariana Duarte de Zamith-Miranda, Daniel Lopez, Pablo H. Rodrigues, Marcio L. Pontes, Bruno Viana, Nathan B. Deleon-Rodriguez, Carlos M. Rossi, Diego Conrado Pereira Casadevall, Arturo Gomes, Andre M. O. Martinez, Luis R. Schnaar, Ronald L. Nosanchuk, Joshua D. Nimrichter, Leonardo |
| dc.subject.mesh.en_US.fl_str_mv |
Animals Cell Adhesion Cell Line Endocytosis Histoplasma Host-Pathogen Interactions Macrophages Membrane Microdomains Mice Inbred C57BL Mice Knockout |
| topic |
Animals Cell Adhesion Cell Line Endocytosis Histoplasma Host-Pathogen Interactions Macrophages Membrane Microdomains Mice Inbred C57BL Mice Knockout Glycosphingolipids Histoplasma capsulatum Lipid rafts Macrophages |
| dc.subject.en.en_US.fl_str_mv |
Glycosphingolipids Histoplasma capsulatum Lipid rafts Macrophages |
| description |
Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University. Niterói, RJ, Brazil / Departments of Medicine. Division of Infectious Diseases and Microbiology and Immunology. Albert Einstein College of Medicine. Bronx, USA. |
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2019 |
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2019 |
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2023-07-13T22:01:41Z |
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2023-07-13T22:01:41Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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GUIMARAES, Allan J. et al. Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages. Cellular Microbiology, v. 21, n. 3, p. 1-18, e12976, Sept. 2019. |
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https://www.arca.fiocruz.br/handle/icict/59576 |
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1462-5814 |
| dc.identifier.doi.none.fl_str_mv |
10.1111/CMI.12976 |
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1462-5822 |
| identifier_str_mv |
GUIMARAES, Allan J. et al. Host membrane glycosphingolipids and lipid microdomains facilitate Histoplasma capsulatum internalisation by macrophages. Cellular Microbiology, v. 21, n. 3, p. 1-18, e12976, Sept. 2019. 1462-5814 10.1111/CMI.12976 1462-5822 |
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Wiley |
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Wiley |
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