Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory

Detalhes bibliográficos
Autor(a) principal: Höfer, Philipp
Data de Publicação: 2011
Outros Autores: Vermette, Patrick, Groleau, Denis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32484
Resumo: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
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spelling Höfer, PhilippVermette, PatrickGroleau, Denis2019-04-15T13:38:46Z2019-04-15T13:38:46Z2011HÖFER, Philipp; VERMETTE, Patrick; GROLEAU, Denis. Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory. Bioengineered Bugs, Austin, v. 2, n. 2, p. 71-79, 2011.1949-1018https://www.arca.fiocruz.br/handle/icict/3248410.4161/bbug.2.2.15009engIntroducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factoryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Université de Sherbrooke. Department of Chemical and Biotechnological Engineering. Laboratoire de Bioingénierie et de Biophysique de l’Université de Sherbrooke. Québec, Canada.Microbial and Enzymatic Technology Group. Bioprocess Centre. Biotechnology Research Institute. National Research Council Canada. Montréal, Québec, Canada.Discussion on and use of methanol as chemical feedstock and as alternative fuel has gained momentum during the past years. Consequently, microorganism and product design based on ‘’methylotrophism’’ is in vogue as reflected by increasing research and development activities in methanol-related areas. A recent example of microorganism and product development is the use of recombinant Methylobacterium extorquens ATCC 55366 strains in the production of second generation biopolyesters. Feeding n-alkenoic acids in addition to methanol yielded functionalized polyhydroxyalkanoates (PHAs) and uncovered how M. extorquens copes with fatty acids. While some parts of the degradation pathway remain unclear, possible metabolic routes are suggested that may explain the significant loss of double bonds prior to polymerization of PHA precursors and occurrence of odd-numbered monomers derived from even-numbered n-alkenoic acids. In addition, microbial discoloration upon fatty acid feeding is discussed and future directions for further genetic modification of M. extorquens are provided.MethylobacteriumMicrobial bioplastic factoryBioengineered buginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/32484/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALF157-Introducing a new Bioengineered Bug Methylobacterium extorquens tuned as a microbial bioplastic factory.pdfapplication/pdf2382920https://www.arca.fiocruz.br/bitstream/icict/32484/2/F157-Introducing%20a%20new%20Bioengineered%20Bug%20Methylobacterium%20extorquens%20tuned%20as%20a%20microbial%20bioplastic%20factory.pdf656126a2ccd1fe6219fec336296addc7MD52TEXTF157-Introducing a new Bioengineered Bug Methylobacterium extorquens tuned as a microbial bioplastic factory.pdf.txtF157-Introducing a new Bioengineered Bug Methylobacterium extorquens tuned as a microbial bioplastic factory.pdf.txtExtracted texttext/plain47069https://www.arca.fiocruz.br/bitstream/icict/32484/3/F157-Introducing%20a%20new%20Bioengineered%20Bug%20Methylobacterium%20extorquens%20tuned%20as%20a%20microbial%20bioplastic%20factory.pdf.txtb1aadd07baa0651cb2c9f77374797878MD53icict/324842019-04-16 02:00:54.09oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-04-16T05:00:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory
title Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory
spellingShingle Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory
Höfer, Philipp
Methylobacterium
Microbial bioplastic factory
Bioengineered bug
title_short Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory
title_full Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory
title_fullStr Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory
title_full_unstemmed Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory
title_sort Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory
author Höfer, Philipp
author_facet Höfer, Philipp
Vermette, Patrick
Groleau, Denis
author_role author
author2 Vermette, Patrick
Groleau, Denis
author2_role author
author
dc.contributor.author.fl_str_mv Höfer, Philipp
Vermette, Patrick
Groleau, Denis
dc.subject.en.pt_BR.fl_str_mv Methylobacterium
Microbial bioplastic factory
Bioengineered bug
topic Methylobacterium
Microbial bioplastic factory
Bioengineered bug
description Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2019-04-15T13:38:46Z
dc.date.available.fl_str_mv 2019-04-15T13:38:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv HÖFER, Philipp; VERMETTE, Patrick; GROLEAU, Denis. Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory. Bioengineered Bugs, Austin, v. 2, n. 2, p. 71-79, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32484
dc.identifier.issn.pt_BR.fl_str_mv 1949-1018
dc.identifier.doi.none.fl_str_mv 10.4161/bbug.2.2.15009
identifier_str_mv HÖFER, Philipp; VERMETTE, Patrick; GROLEAU, Denis. Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory. Bioengineered Bugs, Austin, v. 2, n. 2, p. 71-79, 2011.
1949-1018
10.4161/bbug.2.2.15009
url https://www.arca.fiocruz.br/handle/icict/32484
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