Activation of J77A.1 macrophages by three phospholipases A2 isolated from bothrops atrox snake venom
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14147 http://dx.doi.org/10.1155/2014/683123 |
Resumo: | Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia Aplicada à Saúde. Porto Velho, RO, Brazil. |
| id |
CRUZ_c22a4fbf6840f88efaf2692703d01b20 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/14147 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Furtado, Juliana L.Oliveira, George A.Pontes, Adriana S.Setúbal, Sulamita da S.Xavier, Caroline V.Lacouth-Silva, FabianneLima, Beatriz F.Zaqueo, Kayena D.Kayano, Anderson M.Calderon, Leonardo A.Stábeli, Rodrigo G.Soares, Andreimar M.Zuliani, Juliana P.2016-05-10T14:32:57Z2016-05-10T14:32:57Z2014Zuliani, J. P. et al. Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom. BioMed Research International, v. 2014, p. 1-13, 2014.https://www.arca.fiocruz.br/handle/icict/14147http://dx.doi.org/10.1155/2014/683123engBioMed Research InternationalActivation of J77A.1 macrophages by three phospholipases A2 isolated from bothrops atrox snake venominfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Laboratório de Imunofarmacologia Aplicada à Saúde. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia Aplicada à Saúde. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia Aplicada à Saúde. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia Aplicada à Saúde. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia Aplicada à Saúde. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia Aplicada à Saúde. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia Aplicada à Saúde. Porto Velho, RO, Brazil. / Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia Aplicada à Saúde. Porto Velho, RO, Brazil. / Fundação Oswaldo Cruz. Centro de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.In the present study, we investigated the in vitro effects of two basic myotoxic phospholipases A2 (PLA2), BaTX-I, a catalytically inactive Lys-49 variant, and BaTX-II, a catalytically active Asp-49, and of one acidic myotoxic PLA2, BaPLA2, a catalytically active Asp-49, isolated from Bothrops atrox snake venom, on the activation of J774A.1 macrophages. At noncytotoxic concentrations, the toxins did not affect the adhesion of the macrophages, nor their ability to detach. The data obtained showed that only BaTXI stimulated complement receptor-mediated phagocytosis. However, BaTX-I, BaTX-II, and BaPLA2 induced the release of the superoxide anion by J774A.1 macrophages. Additionally, only BaTX-I raised the lysosomal volume of macrophages after 15 min of incubation. After 30 min, all the phospholipases increased this parameter, which was not observed within 60 min. Moreover, BaTX-I, BaTX-II, and BaPLA2 increased the number of lipid bodies on macrophages submitted to phagocytosis and not submitted to phagocytosis. However, BaTX-II and BaPLA2 induced the release of TNF- by J774A.1 macrophages. Taken together, the data show that, despite differences in enzymatic activity, the three toxins induced inflammatory events and whether the enzyme is acidic or basic does not seem to contribute to these effects.J77A.1Phospholipases A2Bothrops atroxinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14147/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALActivation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom.pdfActivation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom.pdfapplication/pdf2090853https://www.arca.fiocruz.br/bitstream/icict/14147/2/Activation%20of%20J77A.1%20Macrophages%20by%20Three%20Phospholipases%20A2%20Isolated%20from%20Bothrops%20atrox%20Snake%20Venom.pdf0669d064c65a39c61c661542c067ee9bMD52TEXTActivation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom.pdf.txtActivation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom.pdf.txtExtracted texttext/plain67770https://www.arca.fiocruz.br/bitstream/icict/14147/3/Activation%20of%20J77A.1%20Macrophages%20by%20Three%20Phospholipases%20A2%20Isolated%20from%20Bothrops%20atrox%20Snake%20Venom.pdf.txtcc9675c79ded001a0969fb8e20198352MD53icict/141472018-04-02 08:09:21.939oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-02T11:09:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Activation of J77A.1 macrophages by three phospholipases A2 isolated from bothrops atrox snake venom |
| title |
Activation of J77A.1 macrophages by three phospholipases A2 isolated from bothrops atrox snake venom |
| spellingShingle |
Activation of J77A.1 macrophages by three phospholipases A2 isolated from bothrops atrox snake venom Furtado, Juliana L. J77A.1 Phospholipases A2 Bothrops atrox |
| title_short |
Activation of J77A.1 macrophages by three phospholipases A2 isolated from bothrops atrox snake venom |
| title_full |
Activation of J77A.1 macrophages by three phospholipases A2 isolated from bothrops atrox snake venom |
| title_fullStr |
Activation of J77A.1 macrophages by three phospholipases A2 isolated from bothrops atrox snake venom |
| title_full_unstemmed |
Activation of J77A.1 macrophages by three phospholipases A2 isolated from bothrops atrox snake venom |
| title_sort |
Activation of J77A.1 macrophages by three phospholipases A2 isolated from bothrops atrox snake venom |
| author |
Furtado, Juliana L. |
| author_facet |
Furtado, Juliana L. Oliveira, George A. Pontes, Adriana S. Setúbal, Sulamita da S. Xavier, Caroline V. Lacouth-Silva, Fabianne Lima, Beatriz F. Zaqueo, Kayena D. Kayano, Anderson M. Calderon, Leonardo A. Stábeli, Rodrigo G. Soares, Andreimar M. Zuliani, Juliana P. |
| author_role |
author |
| author2 |
Oliveira, George A. Pontes, Adriana S. Setúbal, Sulamita da S. Xavier, Caroline V. Lacouth-Silva, Fabianne Lima, Beatriz F. Zaqueo, Kayena D. Kayano, Anderson M. Calderon, Leonardo A. Stábeli, Rodrigo G. Soares, Andreimar M. Zuliani, Juliana P. |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Furtado, Juliana L. Oliveira, George A. Pontes, Adriana S. Setúbal, Sulamita da S. Xavier, Caroline V. Lacouth-Silva, Fabianne Lima, Beatriz F. Zaqueo, Kayena D. Kayano, Anderson M. Calderon, Leonardo A. Stábeli, Rodrigo G. Soares, Andreimar M. Zuliani, Juliana P. |
| dc.subject.en.pt_BR.fl_str_mv |
J77A.1 Phospholipases A2 Bothrops atrox |
| topic |
J77A.1 Phospholipases A2 Bothrops atrox |
| description |
Fundação Oswaldo Cruz. Laboratório de Imunofarmacologia Aplicada à Saúde. Porto Velho, RO, Brazil. |
| publishDate |
2014 |
| dc.date.issued.fl_str_mv |
2014 |
| dc.date.accessioned.fl_str_mv |
2016-05-10T14:32:57Z |
| dc.date.available.fl_str_mv |
2016-05-10T14:32:57Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
Zuliani, J. P. et al. Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom. BioMed Research International, v. 2014, p. 1-13, 2014. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/14147 |
| dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.1155/2014/683123 |
| identifier_str_mv |
Zuliani, J. P. et al. Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom. BioMed Research International, v. 2014, p. 1-13, 2014. |
| url |
https://www.arca.fiocruz.br/handle/icict/14147 http://dx.doi.org/10.1155/2014/683123 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
BioMed Research International |
| publisher.none.fl_str_mv |
BioMed Research International |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/14147/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/14147/2/Activation%20of%20J77A.1%20Macrophages%20by%20Three%20Phospholipases%20A2%20Isolated%20from%20Bothrops%20atrox%20Snake%20Venom.pdf https://www.arca.fiocruz.br/bitstream/icict/14147/3/Activation%20of%20J77A.1%20Macrophages%20by%20Three%20Phospholipases%20A2%20Isolated%20from%20Bothrops%20atrox%20Snake%20Venom.pdf.txt |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 0669d064c65a39c61c661542c067ee9b cc9675c79ded001a0969fb8e20198352 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1813009180123987968 |