EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46908 |
Resumo: | Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia. Maruipe, Vitória, ES, Brasil. |
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Spano, Liliana CruzGuerrieri, Caroline GastaldiVolpini, Lays Paula BondiSchuenck, Ricardo PintoGoulart, Jaqueline PegorettiBoina, ElizabethRecco, Celia Regina NascimentoRodrigues, Rodrigo RibeiroSantos, Luís Fernando dosFumian, Tulio Machado2021-04-26T15:08:10Z2021-04-26T15:08:10Z2021SPANO, Lilliana Cruz et al. EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019. BMC Microbiology, v. 21, n. 95, 9, 2021.1471-2180https://www.arca.fiocruz.br/handle/icict/4690810.1186/s12866-021-02161-xengBMCEscherichia coli enterohemorrágicaEscherichia coli Shiga-toxigênicaNorovírusSurtosSíndrome hemolítico-urêmicaCrecheEnterohemorrhagic Escherichia coliShiga-toxigenic Escherichia coliNorovirusOutbreaksHemolytic uremic syndromeChild daycare centerEHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia. Maruipe, Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia. Vitória, ES, Brasil.Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia. Vitória, ES, Brasil.Secretaria Estadual de Saúde. Laboratório Central de Saúde Pública. Vitória, ES, Brasil.Secretaria Estadual de Saúde. Laboratório Central de Saúde Pública. Vitória, ES, Brasil.Secretaria Municipal de Saúde. Serviço de Epidemiologia. Vilha Velha, ES, Brasil.Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia. Vitória, ES, Brasil / Secretaria Estadual de Saúde. Laboratório Central de Saúde Pública. Vitória, ES, Brasil.Instituto Adolpho Lutz. Centro de Bacteriologia. Laboratório de Referência Nacional para infecções entéricas por Escherichia coli. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Background: This study describes the investigation of an outbreak of diarrhea, hemorrhagic colitis (HC), and hemolytic uremic syndrome (HUS) at a daycare center in southeastern Brazil, involving fourteen children, six staff members, six family members, and one nurse. All bacterial and viral pathogens detected were genetically characterized. Results: Two isolates of a strain of enterohemorrhagic Escherichia coli (EHEC) serotype O111:H8 were recovered, one implicated in a case of HUS and the other in a case of uncomplicated diarrhea. These isolates had a clonal relationship of 94% and carried the stx2a and eae virulence genes and the OI-122 pathogenicity island. The EHEC strain was determined to be a single-locus variant of sequence type (ST) 327. EHEC isolates were resistant to ofloxacin, doxycycline, tetracycline, ampicillin, and trimethoprim-sulfamethoxazole and intermediately resistant to levofloxacin and ciprofloxacin. Rotavirus was not detected in any samples, and norovirus was detected in 46.7% (14/30) of the stool samples, three of which were from asymptomatic staff members. The noroviruses were classified as the recombinant GII.4 Sydney [P16] by gene sequencing. Conclusion: In this outbreak, it was possible to identify an uncommon stx2a + EHEC O111:H8 strain, and the most recent pandemic norovirus strain GII.4 Sydney [P16]. Our findings reinforce the need for surveillance and diagnosis of multiple enteric pathogens by public health authorities, especially during outbreaks.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46908/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALTulioMachado_Fumian_etal_IOC_2021.pdfTulioMachado_Fumian_etal_IOC_2021.pdfapplication/pdf1492805https://www.arca.fiocruz.br/bitstream/icict/46908/2/TulioMachado_Fumian_etal_IOC_2021.pdf1c4a6d0ec6104a86ab50492fa3d8e79cMD52TEXTTulioMachado_Fumian_etal_IOC_2021.pdf.txtTulioMachado_Fumian_etal_IOC_2021.pdf.txtExtracted texttext/plain47031https://www.arca.fiocruz.br/bitstream/icict/46908/3/TulioMachado_Fumian_etal_IOC_2021.pdf.txte12514384a03ee8ee2b7adac22fea165MD53icict/469082022-06-24 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| dc.title.pt_BR.fl_str_mv |
EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019 |
| title |
EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019 |
| spellingShingle |
EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019 Spano, Liliana Cruz Escherichia coli enterohemorrágica Escherichia coli Shiga-toxigênica Norovírus Surtos Síndrome hemolítico-urêmica Creche Enterohemorrhagic Escherichia coli Shiga-toxigenic Escherichia coli Norovirus Outbreaks Hemolytic uremic syndrome Child daycare center |
| title_short |
EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019 |
| title_full |
EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019 |
| title_fullStr |
EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019 |
| title_full_unstemmed |
EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019 |
| title_sort |
EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019 |
| author |
Spano, Liliana Cruz |
| author_facet |
Spano, Liliana Cruz Guerrieri, Caroline Gastaldi Volpini, Lays Paula Bondi Schuenck, Ricardo Pinto Goulart, Jaqueline Pegoretti Boina, Elizabeth Recco, Celia Regina Nascimento Rodrigues, Rodrigo Ribeiro Santos, Luís Fernando dos Fumian, Tulio Machado |
| author_role |
author |
| author2 |
Guerrieri, Caroline Gastaldi Volpini, Lays Paula Bondi Schuenck, Ricardo Pinto Goulart, Jaqueline Pegoretti Boina, Elizabeth Recco, Celia Regina Nascimento Rodrigues, Rodrigo Ribeiro Santos, Luís Fernando dos Fumian, Tulio Machado |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Spano, Liliana Cruz Guerrieri, Caroline Gastaldi Volpini, Lays Paula Bondi Schuenck, Ricardo Pinto Goulart, Jaqueline Pegoretti Boina, Elizabeth Recco, Celia Regina Nascimento Rodrigues, Rodrigo Ribeiro Santos, Luís Fernando dos Fumian, Tulio Machado |
| dc.subject.other.pt_BR.fl_str_mv |
Escherichia coli enterohemorrágica Escherichia coli Shiga-toxigênica Norovírus Surtos Síndrome hemolítico-urêmica Creche |
| topic |
Escherichia coli enterohemorrágica Escherichia coli Shiga-toxigênica Norovírus Surtos Síndrome hemolítico-urêmica Creche Enterohemorrhagic Escherichia coli Shiga-toxigenic Escherichia coli Norovirus Outbreaks Hemolytic uremic syndrome Child daycare center |
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Enterohemorrhagic Escherichia coli Shiga-toxigenic Escherichia coli Norovirus Outbreaks Hemolytic uremic syndrome Child daycare center |
| description |
Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Departamento de Patologia. Maruipe, Vitória, ES, Brasil. |
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2021 |
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2021-04-26T15:08:10Z |
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2021-04-26T15:08:10Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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SPANO, Lilliana Cruz et al. EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019. BMC Microbiology, v. 21, n. 95, 9, 2021. |
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https://www.arca.fiocruz.br/handle/icict/46908 |
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1471-2180 |
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10.1186/s12866-021-02161-x |
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SPANO, Lilliana Cruz et al. EHEC O111:H8 strain and norovirus GII.4 Sydney [P16] causing an outbreak in a daycare center, Brazil, 2019. BMC Microbiology, v. 21, n. 95, 9, 2021. 1471-2180 10.1186/s12866-021-02161-x |
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