A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.

Detalhes bibliográficos
Autor(a) principal: Barral Netto, Manoel
Data de Publicação: 1986
Outros Autores: Barral, Aldina Maria Prado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7467
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Prof. Edgard Santos. Salvador, BA, Brasil
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spelling Barral Netto, ManoelBarral, Aldina Maria Prado2014-03-28T13:43:05Z2014-03-28T13:43:05Z1986BARRAL-NETTO, M.; BARRAL, A. A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding. Memórias do Instituto Oswaldo Cruz, v. 81, n. 3, p. 343-345, 1986.0074-0276https://www.arca.fiocruz.br/handle/icict/7467engFiocruzA simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Prof. Edgard Santos. Salvador, BA, BrasilWe report a simple method for evaluating the binding of concanavalin A (ConA) to human peripheral blood mononuclear cells (PBMC). The binding is evidenced by an immunoenzymic assay using peroxidase-conjugated immunoglobulins of a rabbit anti-ConA serum. Using the method we show that sera from patients with American leishmaniasis do not interfere with binding of ConA to PBMC.Concanavalin AMononuclear cellsVisceral leishmaniasisConcanavalina A/metabolismoLeishmaniose Visceral/sangueMonócitos/metabolismoTécnicas ImunoenzimáticasHumanosLigação Proteicainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBarral-Netto A simple method for....pdfBarral-Netto A simple method for....pdfapplication/pdf116108https://www.arca.fiocruz.br/bitstream/icict/7467/1/Barral-Netto%20A%20simple%20method%20for....pdfd864c749880bd68721cda93a62517e89MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7467/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBarral-Netto A simple method for....pdf.txtBarral-Netto A simple method for....pdf.txtExtracted texttext/plain3https://www.arca.fiocruz.br/bitstream/icict/7467/5/Barral-Netto%20A%20simple%20method%20for....pdf.txt2228e977ebea8966e27929f43e39cb67MD55THUMBNAILBarral-Netto A simple method for....pdf.jpgBarral-Netto A simple method for....pdf.jpgGenerated Thumbnailimage/jpeg2676https://www.arca.fiocruz.br/bitstream/icict/7467/4/Barral-Netto%20A%20simple%20method%20for....pdf.jpg1ee5d94101ae578e1f326c3026b066c1MD54icict/74672023-03-15 14:33:10.493oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.
title A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.
spellingShingle A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.
Barral Netto, Manoel
Concanavalin A
Mononuclear cells
Visceral leishmaniasis
Concanavalina A/metabolismo
Leishmaniose Visceral/sangue
Monócitos/metabolismo
Técnicas Imunoenzimáticas
Humanos
Ligação Proteica
title_short A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.
title_full A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.
title_fullStr A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.
title_full_unstemmed A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.
title_sort A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding.
author Barral Netto, Manoel
author_facet Barral Netto, Manoel
Barral, Aldina Maria Prado
author_role author
author2 Barral, Aldina Maria Prado
author2_role author
dc.contributor.author.fl_str_mv Barral Netto, Manoel
Barral, Aldina Maria Prado
dc.subject.en.pt_BR.fl_str_mv Concanavalin A
Mononuclear cells
Visceral leishmaniasis
topic Concanavalin A
Mononuclear cells
Visceral leishmaniasis
Concanavalina A/metabolismo
Leishmaniose Visceral/sangue
Monócitos/metabolismo
Técnicas Imunoenzimáticas
Humanos
Ligação Proteica
dc.subject.decs.pt_BR.fl_str_mv Concanavalina A/metabolismo
Leishmaniose Visceral/sangue
Monócitos/metabolismo
Técnicas Imunoenzimáticas
Humanos
Ligação Proteica
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Prof. Edgard Santos. Salvador, BA, Brasil
publishDate 1986
dc.date.issued.fl_str_mv 1986
dc.date.accessioned.fl_str_mv 2014-03-28T13:43:05Z
dc.date.available.fl_str_mv 2014-03-28T13:43:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
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dc.identifier.citation.fl_str_mv BARRAL-NETTO, M.; BARRAL, A. A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding. Memórias do Instituto Oswaldo Cruz, v. 81, n. 3, p. 343-345, 1986.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7467
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv BARRAL-NETTO, M.; BARRAL, A. A simple method for assessing the binding of concanavalin A to mononuclear cell surfaces: no interference of visceral leishmaniasis serum on this binding. Memórias do Instituto Oswaldo Cruz, v. 81, n. 3, p. 343-345, 1986.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/7467
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dc.publisher.none.fl_str_mv Fiocruz
publisher.none.fl_str_mv Fiocruz
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