The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon gene

Detalhes bibliográficos
Autor(a) principal: Fernandes, O.
Data de Publicação: 1999
Outros Autores: Mangia, R. H., Lisboa, C. V., Pinho, A. P., Morel, Carlos Medicis, Zingales, B., Campbell, D. A., Jansen, A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14702
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
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spelling Fernandes, O.Mangia, R. H.Lisboa, C. V.Pinho, A. P.Morel, Carlos MedicisZingales, B.Campbell, D. A.Jansen, A. M.2016-06-30T13:17:06Z2016-07-07T12:22:54Z2016-06-30T13:17:06Z2016-07-07T12:22:54Z1999FERNANDES, O. et al. The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the non-transcribed spacer of the mini-exon gene. Parasitology, v. 118, n. 2, p. 161-166, fev. 1999.0031-1820https://www.arca.fiocruz.br/handle/icict/1470210.1017/s0031182098003709engCambridge University PressTrypanosoma cruziCiclo silvestreRio de JaneiroTrypanosoma cruziMini-exon geneSylvatic cycleDidelphis marsupialisLeontophitecus rosaliaRio de JaneiroThe complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon geneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Instituto de Química. Departamento de Bioquímica. São Paulo, SP, Brasil.UCLA School of Medicine. Department of Microbiology and Immunology. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.American trypanosamiasis occurs in nature as a sylvatic cycle, where Trypanosoma cruzi interacts with wild triatomines and mammalian reservoirs, such as marsupials, rodents, armadillos and other animals. Due to difficulties in trying to isolate T. cruzi stocks from the sylvatic cycle, very few studies have been performed in order to understand the parasite infection in natural environments. Traditionally T. cruzi has been considered to be composed of a highly heterogeneous population of parasites. In contrast, the mini-exon and the 24Sα rRNA gene loci have shown that T. cruzi stocks can be clustered in 2 major phylogenetic groups: lineage 1 and lineage 2. In this report, 68 recently isolated T. cruzi samples from the sylvatic cycle belonging to different geographical areas in Rio de Janeiro, Brazil, have been typed based on a variable spot in the non-transcribed spacer of the mini-exon gene. Eight isolates were from triatomines, 26 stocks were from golden-lion tamarins, 31 from opossums, 2 from rodents and 1 from a three-toed sloth. Thirty (44% – 30}68) isolates were typed as lineage 1, while 36 (53% – 36}68) isolates were typed as lineage 2. Two opossums presented mixed infection. Therefore, 3% (2}68) of the isolates were typed as lineage 1­lineage 2. Using these geographical regions as models of sylvatic environments, it was observed that 96% of the Didelphis marsupialis were infected by lineage 2 isolates, while all 26 golden-lion tamarins were infected by lineage 1. The results show preferential association of the 2 lineages of T. cruzi with different hosts, composing the complexity of the sylvatic cycle.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTcarlos30_morel_etal_IOC_1999.pdf.txtcarlos30_morel_etal_IOC_1999.pdf.txtExtracted texttext/plain25469https://www.arca.fiocruz.br/bitstream/icict/14702/4/carlos30_morel_etal_IOC_1999.pdf.txtfb0a4692fbb239b2e72944428e924434MD54ORIGINALcarlos30_morel_etal_IOC_1999.pdfapplication/pdf194555https://www.arca.fiocruz.br/bitstream/icict/14702/2/carlos30_morel_etal_IOC_1999.pdf292976e57d5190c6fb030f10038c0b36MD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14702/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/147022023-11-09 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dc.title.pt_BR.fl_str_mv The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon gene
title The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon gene
spellingShingle The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon gene
Fernandes, O.
Trypanosoma cruzi
Ciclo silvestre
Rio de Janeiro
Trypanosoma cruzi
Mini-exon gene
Sylvatic cycle
Didelphis marsupialis
Leontophitecus rosalia
Rio de Janeiro
title_short The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon gene
title_full The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon gene
title_fullStr The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon gene
title_full_unstemmed The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon gene
title_sort The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the nontranscribed spacer of the mini-exon gene
author Fernandes, O.
author_facet Fernandes, O.
Mangia, R. H.
Lisboa, C. V.
Pinho, A. P.
Morel, Carlos Medicis
Zingales, B.
Campbell, D. A.
Jansen, A. M.
author_role author
author2 Mangia, R. H.
Lisboa, C. V.
Pinho, A. P.
Morel, Carlos Medicis
Zingales, B.
Campbell, D. A.
Jansen, A. M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, O.
Mangia, R. H.
Lisboa, C. V.
Pinho, A. P.
Morel, Carlos Medicis
Zingales, B.
Campbell, D. A.
Jansen, A. M.
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Ciclo silvestre
Rio de Janeiro
topic Trypanosoma cruzi
Ciclo silvestre
Rio de Janeiro
Trypanosoma cruzi
Mini-exon gene
Sylvatic cycle
Didelphis marsupialis
Leontophitecus rosalia
Rio de Janeiro
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Mini-exon gene
Sylvatic cycle
Didelphis marsupialis
Leontophitecus rosalia
Rio de Janeiro
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.
publishDate 1999
dc.date.issued.fl_str_mv 1999
dc.date.accessioned.fl_str_mv 2016-06-30T13:17:06Z
2016-07-07T12:22:54Z
dc.date.available.fl_str_mv 2016-06-30T13:17:06Z
2016-07-07T12:22:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERNANDES, O. et al. The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the non-transcribed spacer of the mini-exon gene. Parasitology, v. 118, n. 2, p. 161-166, fev. 1999.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14702
dc.identifier.issn.pt_BR.fl_str_mv 0031-1820
dc.identifier.doi.pt_BR.fl_str_mv 10.1017/s0031182098003709
identifier_str_mv FERNANDES, O. et al. The complexity of the sylvatic cycle of Trypanosoma cruzi in Rio de Janeiro state (Brazil) revealed by the non-transcribed spacer of the mini-exon gene. Parasitology, v. 118, n. 2, p. 161-166, fev. 1999.
0031-1820
10.1017/s0031182098003709
url https://www.arca.fiocruz.br/handle/icict/14702
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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