1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients

Detalhes bibliográficos
Autor(a) principal: El-Bacha, Tatiana
Data de Publicação: 2016
Outros Autores: Struchiner, Claudio J., Cordeiro, Marli Tenorio, Almeida, Fabio C. L., Marques, Ernesto Torres, Da Poian, Andrea T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19389
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brazil.
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spelling El-Bacha, TatianaStruchiner, Claudio J.Cordeiro, Marli TenorioAlmeida, Fabio C. L.Marques, Ernesto TorresDa Poian, Andrea T.2017-06-14T14:18:51Z2017-06-14T14:18:51Z2016EL-BACHA, Tatiana et al. 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients. Journal of Virology, v. 90, n. 16, p. 7429-7443, Aug. 2016.1098-5514https://www.arca.fiocruz.br/handle/icict/1938910.1128/JVI.00187-16engDengueMortalidadeDiagnóstico precoceDengue / complicaçõesDengue / patologiaHepatopatias / diagnósticoEspectroscopia de Ressonância MagnéticaMetabolômicaPlasma / Química1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Doenças Endêmicas Samuel Pessoa. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Centro Nacional de Biologia Estrutural e Bioimagem. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / University of Pittsburgh. Center for Vaccine Research. Pittsburgh, Pennsylvania, USA.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brazil.Dengue, due to its global burden, is the most important arthropod-borne flavivirus disease, and early detection lowers fatality rates to below 1%. Since the metabolic resources crucial for viral replication are provided by host cells, detection of changes in the metabolic profile associated with disease pathogenesis could help with the identification of markers of prognostic and diagnostic importance. We applied (1)H nuclear magnetic resonance exploratory metabolomics to study longitudinal changes in plasma metabolites in a cohort in Recife, Brazil. To gain statistical power, we used innovative paired multivariate analyses to discriminate individuals with primary and secondary infection presenting as dengue fever (DF; mild) and dengue hemorrhagic fever (DHF; severe) and subjects with a nonspecific nondengue (ND) illness (ND subjects). Our results showed that a decrease in plasma low-density lipoprotein (LDL) and very-low-density lipoprotein (VLDL) discriminated dengue virus (DENV)-infected subjects from ND subjects, and also, subjects with severe infection even presented a decrease in lipoprotein concentrations compared to the concentrations in subjects with mild infection. These results add to the ongoing discussion that the manipulation of lipid metabolism is crucial for DENV replication and infection. In addition, a decrease in plasma glutamine content was characteristic of DENV infection and disease severity, and an increase in plasma acetate levels discriminated subjects with DF and DHF from ND subjects. Several other metabolites shown to be altered in DENV infection and the implications of these alterations are discussed. We hypothesize that these changes in the plasma metabolome are suggestive of liver dysfunction, could provide insights into the underlying molecular mechanisms of dengue virus pathogenesis, and could help to discriminate individuals at risk of the development of severe infection and predict disease outcome.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19389/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL27279613 2016 elb-1hn.oa.pdf27279613 2016 elb-1hn.oa.pdfapplication/pdf3586657https://www.arca.fiocruz.br/bitstream/icict/19389/2/27279613%202016%20elb-1hn.oa.pdf53670e716bbedf54ebba0642ce474924MD52TEXT27279613 2016 elb-1hn.oa.pdf.txt27279613 2016 elb-1hn.oa.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients
title 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients
spellingShingle 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients
El-Bacha, Tatiana
Dengue
Mortalidade
Diagnóstico precoce
Dengue / complicações
Dengue / patologia
Hepatopatias / diagnóstico
Espectroscopia de Ressonância Magnética
Metabolômica
Plasma / Química
title_short 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients
title_full 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients
title_fullStr 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients
title_full_unstemmed 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients
title_sort 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients
author El-Bacha, Tatiana
author_facet El-Bacha, Tatiana
Struchiner, Claudio J.
Cordeiro, Marli Tenorio
Almeida, Fabio C. L.
Marques, Ernesto Torres
Da Poian, Andrea T.
author_role author
author2 Struchiner, Claudio J.
Cordeiro, Marli Tenorio
Almeida, Fabio C. L.
Marques, Ernesto Torres
Da Poian, Andrea T.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv El-Bacha, Tatiana
Struchiner, Claudio J.
Cordeiro, Marli Tenorio
Almeida, Fabio C. L.
Marques, Ernesto Torres
Da Poian, Andrea T.
dc.subject.other.none.fl_str_mv Dengue
Mortalidade
Diagnóstico precoce
topic Dengue
Mortalidade
Diagnóstico precoce
Dengue / complicações
Dengue / patologia
Hepatopatias / diagnóstico
Espectroscopia de Ressonância Magnética
Metabolômica
Plasma / Química
dc.subject.decs.none.fl_str_mv Dengue / complicações
Dengue / patologia
Hepatopatias / diagnóstico
Espectroscopia de Ressonância Magnética
Metabolômica
Plasma / Química
description Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Rio de Janeiro, RJ, Brazil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-06-14T14:18:51Z
dc.date.available.fl_str_mv 2017-06-14T14:18:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv EL-BACHA, Tatiana et al. 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients. Journal of Virology, v. 90, n. 16, p. 7429-7443, Aug. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19389
dc.identifier.issn.pt_BR.fl_str_mv 1098-5514
dc.identifier.doi.pt_BR.fl_str_mv 10.1128/JVI.00187-16
identifier_str_mv EL-BACHA, Tatiana et al. 1H Nuclear Magnetic Resonance Metabolomics of Plasma Unveils Liver Dysfunction in Dengue Patients. Journal of Virology, v. 90, n. 16, p. 7429-7443, Aug. 2016.
1098-5514
10.1128/JVI.00187-16
url https://www.arca.fiocruz.br/handle/icict/19389
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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