Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42323 |
Resumo: | Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular. Viçosa, MG, Brasil |
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Ferreira, Priscila GonçalvesTesla, BlankaHorácio, Elvira Cynthia AlvesNahum, Laila AlvesBrindley, Melinda AnnMendes, Tiago Antônio de OliveiraMurdock, Courtney Cuinn2020-07-21T14:45:26Z2020-07-21T14:45:26Z2020FERREIRA, Priscila Goncalves et al. Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions. Frontiers In Microbiology, v. 11, 901, 20201664-302Xhttps://www.arca.fiocruz.br/handle/icict/4232310.3389/fmicb.2020.00901porScimagoTemperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular. Viçosa, MG, BrasilDepartment of Infectious Diseases. College of Veterinary Medicine. University of Georgia. Athens, GA, United States of AmericaFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Genética, Ecologia e Evolução. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Genética, Ecologia e Evolução. Belo Horizonte, MG, Brasil/Promove Colégio de Tecnologia. Belo Horizonte, MG, BrazilDepartment of Infectious Diseases. College of Veterinary Medicine. University of Georgia. Athens, GA, United States of America/Department of Population Health. College of Veterinary Medicine. University of Georgia. Athens, GA, United States of America/Center for Vaccines and Immunology. University of Georgia. Athens, GA, United States of AmericaUniversidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular. Viçosa, MG, BrasilDepartment of Infectious Diseases. College of Veterinary Medicine. University of Georgia. Athens, GA, United States of America/Center for Vaccines and Immunology. University of Georgia. Athens, GA, United States of America/Odum School of Ecology. University of Georgia. Athens, GA, United States of America/Center for the Ecology of Infectious Diseases. University of Georgia, Athens. GA, United States of America/Center for Emerging and Global Tropical Diseases. University of Georgia. Athens, GA, United States of America/River Basin Center. University of Georgia. Athens, GA, United States of America/Department of Entomology. College of Agriculture and Life Sciences. Cornell University., Ithaca, NY, United States of AmericaVector-borne flaviviruses are emerging threats to human health. For successful transmission, the virus needs to efficiently enter mosquito cells, replicate within, and escape several tissue barriers while mosquitoes elicit major transcriptional responses to flavivirus infection. This process will not only be affected by the specific mosquito-pathogen pairing, but also variation in key environmental variables such as temperature. Thus far, few studies have examined the molecular responses triggered by temperature and how these responses modify infection outcomes despite substantial evidence showing strong relationships between temperature and transmission in a diversity of systems. To define the host transcriptional changes associated with temperature variation during the early infection process, we compared the transcriptome of mosquito midgut samples from mosquitoes exposed to Zika virus (ZIKV) and non-exposed mosquitoes housed at three different temperatures (20, 28, and 36°C). While the high temperature samples did not have significant changes from standard rearing conditions (28°C) 48 hr post-exposure, the transcriptome profile of mosquitoes housed at 20°C was dramatically different. The expression of genes most altered by the cooler temperature involved aspects of blood-meal digestion, ROS metabolism, and mosquito innate immunity. Further, we did not find significant differences in the viral RNA copy number between 24 and 48 hr post-exposure at 20°C, suggesting ZIKV replication is limited by cold-induced changes to the mosquito midgut environment. In ZIKV-exposed mosquitoes, vitellogenin, a lipid carrier protein, was the most up-regulated at 20°C. Our results provide a deeper understanding of the temperature-triggered transcriptional changes in Aedes aegypti and can be used to further define the molecular mechanisms driven by environmental temperature variation.temperatureAedes aegyptiZika virusRNA-seqtranscriptomeimmune responseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/42323/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALTemperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito–Zika Virus Interactions.pdfTemperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito–Zika Virus Interactions.pdfapplication/pdf2942245https://www.arca.fiocruz.br/bitstream/icict/42323/2/Temperature%20Dramatically%20Shapes%20Mosquito%20Gene%20Expression%20With%20Consequences%20for%20Mosquito%e2%80%93Zika%20Virus%20Interactions.pdf9474615243cdb95c81d9646b775b7c45MD52TEXTTemperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito–Zika Virus Interactions.pdf.txtTemperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito–Zika Virus Interactions.pdf.txtExtracted texttext/plain112671https://www.arca.fiocruz.br/bitstream/icict/42323/3/Temperature%20Dramatically%20Shapes%20Mosquito%20Gene%20Expression%20With%20Consequences%20for%20Mosquito%e2%80%93Zika%20Virus%20Interactions.pdf.txt719c768fa42917749a45b8b8937fdd22MD53icict/423232020-07-22 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| dc.title.pt_BR.fl_str_mv |
Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions |
| title |
Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions |
| spellingShingle |
Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions Ferreira, Priscila Gonçalves temperature Aedes aegypti Zika virus RNA-seq transcriptome immune response |
| title_short |
Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions |
| title_full |
Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions |
| title_fullStr |
Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions |
| title_full_unstemmed |
Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions |
| title_sort |
Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions |
| author |
Ferreira, Priscila Gonçalves |
| author_facet |
Ferreira, Priscila Gonçalves Tesla, Blanka Horácio, Elvira Cynthia Alves Nahum, Laila Alves Brindley, Melinda Ann Mendes, Tiago Antônio de Oliveira Murdock, Courtney Cuinn |
| author_role |
author |
| author2 |
Tesla, Blanka Horácio, Elvira Cynthia Alves Nahum, Laila Alves Brindley, Melinda Ann Mendes, Tiago Antônio de Oliveira Murdock, Courtney Cuinn |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Ferreira, Priscila Gonçalves Tesla, Blanka Horácio, Elvira Cynthia Alves Nahum, Laila Alves Brindley, Melinda Ann Mendes, Tiago Antônio de Oliveira Murdock, Courtney Cuinn |
| dc.subject.en.pt_BR.fl_str_mv |
temperature Aedes aegypti Zika virus RNA-seq transcriptome immune response |
| topic |
temperature Aedes aegypti Zika virus RNA-seq transcriptome immune response |
| description |
Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular. Viçosa, MG, Brasil |
| publishDate |
2020 |
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2020-07-21T14:45:26Z |
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2020-07-21T14:45:26Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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FERREIRA, Priscila Goncalves et al. Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions. Frontiers In Microbiology, v. 11, 901, 2020 |
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https://www.arca.fiocruz.br/handle/icict/42323 |
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1664-302X |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fmicb.2020.00901 |
| identifier_str_mv |
FERREIRA, Priscila Goncalves et al. Temperature Dramatically Shapes Mosquito Gene Expression With Consequences for Mosquito-Zika Virus Interactions. Frontiers In Microbiology, v. 11, 901, 2020 1664-302X 10.3389/fmicb.2020.00901 |
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