Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1111/odi.13988 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/50882 |
Resumo: | Universidade do Estado do Rio de Janeiro. Escola de Odontologia. Departamento de Periondontologia. Rio de Janeiro, RJ, Brasil. |
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Marques, Barbara Bruno FagundesGuimarães, Taísa CoelhoFischer, Ricardo GuimarãesTinoco, Justine Monteiro MonneratPires, Fábio RamoaLima Junior, Josué da CostaStevens, Roy H.Tinoco, Eduardo Muniz Barretto2022-01-25T18:17:20Z2022-01-25T18:17:20Z2021MARQUES, Barbara Bruno Fagundes et al. Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study. Oral Diseases, 00, p. 1 - 6, July 2021.1601-0825https://www.arca.fiocruz.br/handle/icict/5088210.1111/odi.13988engWileyCélulas epiteliaisSARS-CoV- 2Proteína de picoRT-PCREpithelial cellsRT-PCRSARS-CoV- 2Spike proteinReação em Cadeia da Polimerase Via Transcriptase ReversaMorphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Escola de Odontologia. Departamento de Periondontologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Escola de Odontologia. Departamento de Periondontologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Escola de Odontologia. Departamento de Periondontologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Faculdade de Odontologia. Departamento de Clínica Dental. Rio de Janeiro, RJ, Brasil.Universidade Estácio de Sá. Escola de Odontologia. Patologia Oral. Rio de Janeiro, RJ, Brasil / Universidade Estácio de Sá. Programa de Pós-Graduação em Odontologia. Rio de Janeiro, RJ, Brasil.Universidade Estácio de Sá. Programa de Pós-Graduação em Odontologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Laboratory of Oral Infectious Diseases, Maurice Kornberg School of Dentistry. Temple University, Philadelphia, PA, USA.Universidade do Estado do Rio de Janeiro. Escola de Odontologia. Departamento de Periondontologia. Rio de Janeiro, RJ, Brasil.Objective: The aim of the present case–control study was to evaluate the morphological aspects of the epithelial cells from the dorsum of the tongue and the expression of the SARS-CoV- 2 Spike protein in these cells, in patients with and without COVID-19 infection. Methods: 24 individuals with at least one symptom of COVID-19 were recruited among inpatients from Hospital Universitário Pedro Ernesto (Rio de Janeiro, Brazil). 14 patients who tested positive for COVID-19 by RT-PCR were included in the case group, and 10 patients who tested negative were included in the control group. Cytological smears from the dorsum of the tongue were obtained from all patients and analyzed using immunohistochemistry directed against SARS-CoV- 2- Spike protein. Morphological changes in epithelial cells were analyzed using light microscopy. Results: Immunohistochemistry showed that 71% of the COVID-19 patients presented epithelial cells positive for the presence of the SARS-CoV- 2 Spike protein, and all cells coming from patients in the control group were negative. Cytological analysis showed significant differences when comparing epithelial cells from COVID-19- positive and COVID-19- negative patients. Conclusion: COVID-19 may generate dimensional changes in tongue epithelial cells; however, further studies are necessary to understand how this happens.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50882/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALJosueCLimaJunior_TaisaGuimarães_etal_IOC_2021.pdfJosueCLimaJunior_TaisaGuimarães_etal_IOC_2021.pdfapplication/pdf698085https://www.arca.fiocruz.br/bitstream/icict/50882/2/JosueCLimaJunior_TaisaGuimar%c3%a3es_etal_IOC_2021.pdfacb52068b913ce98bc81c0d8ec3b396bMD52icict/508822022-06-24 13:04:24.552oai:www.arca.fiocruz.br:icict/50882Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:04:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study |
| title |
Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study |
| spellingShingle |
Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study Marques, Barbara Bruno Fagundes Células epiteliais SARS-CoV- 2 Proteína de pico RT-PCR Epithelial cells RT-PCR SARS-CoV- 2 Spike protein Reação em Cadeia da Polimerase Via Transcriptase Reversa |
| title_short |
Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study |
| title_full |
Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study |
| title_fullStr |
Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study |
| title_full_unstemmed |
Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study |
| title_sort |
Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study |
| author |
Marques, Barbara Bruno Fagundes |
| author_facet |
Marques, Barbara Bruno Fagundes Guimarães, Taísa Coelho Fischer, Ricardo Guimarães Tinoco, Justine Monteiro Monnerat Pires, Fábio Ramoa Lima Junior, Josué da Costa Stevens, Roy H. Tinoco, Eduardo Muniz Barretto |
| author_role |
author |
| author2 |
Guimarães, Taísa Coelho Fischer, Ricardo Guimarães Tinoco, Justine Monteiro Monnerat Pires, Fábio Ramoa Lima Junior, Josué da Costa Stevens, Roy H. Tinoco, Eduardo Muniz Barretto |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Marques, Barbara Bruno Fagundes Guimarães, Taísa Coelho Fischer, Ricardo Guimarães Tinoco, Justine Monteiro Monnerat Pires, Fábio Ramoa Lima Junior, Josué da Costa Stevens, Roy H. Tinoco, Eduardo Muniz Barretto |
| dc.subject.other.pt_BR.fl_str_mv |
Células epiteliais SARS-CoV- 2 Proteína de pico RT-PCR |
| topic |
Células epiteliais SARS-CoV- 2 Proteína de pico RT-PCR Epithelial cells RT-PCR SARS-CoV- 2 Spike protein Reação em Cadeia da Polimerase Via Transcriptase Reversa |
| dc.subject.en.pt_BR.fl_str_mv |
Epithelial cells RT-PCR SARS-CoV- 2 Spike protein |
| dc.subject.decs.pt_BR.fl_str_mv |
Reação em Cadeia da Polimerase Via Transcriptase Reversa |
| description |
Universidade do Estado do Rio de Janeiro. Escola de Odontologia. Departamento de Periondontologia. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021 |
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2022-01-25T18:17:20Z |
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2022-01-25T18:17:20Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MARQUES, Barbara Bruno Fagundes et al. Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study. Oral Diseases, 00, p. 1 - 6, July 2021. |
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https://www.arca.fiocruz.br/handle/icict/50882 |
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1601-0825 |
| dc.identifier.doi.none.fl_str_mv |
10.1111/odi.13988 |
| identifier_str_mv |
MARQUES, Barbara Bruno Fagundes et al. Morphological alterations in tongue epithelial cells infected by SARS-CoV- 2: A case–control study. Oral Diseases, 00, p. 1 - 6, July 2021. 1601-0825 10.1111/odi.13988 |
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