Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions.

Detalhes bibliográficos
Autor(a) principal: Cruz, A. M.
Data de Publicação: 2005
Outros Autores: Bertho, A. L., Oliveira Neto, M. P., Coutinho, S. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56899
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.
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spelling Cruz, A. M.Bertho, A. L.Oliveira Neto, M. P.Coutinho, S. G.2023-02-06T19:30:01Z2023-02-06T19:30:01Z2005CRUZ, A. M. et al. Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions. British Journal of Dermatology, Grã-Bretanha, v. 153, p. 537-543, 2005.0366-077Xhttps://www.arca.fiocruz.br/handle/icict/5689910.1111/j.1365-2133.2005.06647.xengBritish Association of DermatologistsFlow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.Background CD4+ and CD8+ T lymphocytes play different roles in the outcome of leishmaniasis. However, T-cell distribution in lesions shows significant variability in in situ immunocytochemical studies. Objectives In this report flow cytometry was used to determine the predominant T-cell subsets in leishmaniasis lesions, and their relationship with Leishmaniaresponsive circulating T cells. Patients and methods Mononuclear cells from lesions or peripheral blood (PBMC) of 34 cutaneous (CL), four mucosal (ML) and four disseminated leishmaniasis were phenotypically characterized by flow cytometry. Leishmania-responsive T cells were obtained after in vitro stimulation of PBMC with leishmanial antigens. Results ⁄conclusions Variable amounts of cd lymphocytes were present in all lesions, with no association with duration of illness. The highest percentages of interleukin2R- and interferon-cR-positive cells were observed in ML lesions and could render these T cells more susceptible to the effects of these cytokines. The distribution of intralesional T-lymphocyte subsets was quite variable (CD4+ > CD8+ ¼ 18 cases, CD8+ > CD4+ ¼ 12 cases and CD4+ @ CD8+ ¼ 4 cases) without any association with clinical parameters, and could explain the controversy regarding proportions of these T-cell subsets in leishmaniasis lesions. Low percentages of Leishmania-reactive CD8+ T cells were observed in blood while an enrichment of CD8+ cells was shown in the inflammatory infiltrate, suggesting that local immunoregulatory factors could favour the recruitment and ⁄or proliferation of local CD8+ lymphocytes. Increased percentages of CD8+ cells observed in older lesions are consistent with the hypothesis that they can mediate healing, although their involvement in tissue damage cannot be ruled out. It is possible that these mechanisms can influence the clinical outcome or even the response to therapy.Flow cytometryGamma-deltaInterferon-gammaRInterleukin-2RLeishmaniasis lesionsT lymphocytesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCruz_A.M._etal_INI_IOC_2005.pdfCruz_A.M._etal_INI_IOC_2005.pdfapplication/pdf177872https://www.arca.fiocruz.br/bitstream/icict/56899/2/Cruz_A.M._etal_INI_IOC_2005.pdf84e63538fef19e0d5fbe594e0588259bMD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/56899/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/568992023-09-04 11:30:20.813oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:30:20Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions.
title Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions.
spellingShingle Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions.
Cruz, A. M.
Flow cytometry
Gamma-delta
Interferon-gammaR
Interleukin-2R
Leishmaniasis lesions
T lymphocytes
title_short Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions.
title_full Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions.
title_fullStr Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions.
title_full_unstemmed Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions.
title_sort Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions.
author Cruz, A. M.
author_facet Cruz, A. M.
Bertho, A. L.
Oliveira Neto, M. P.
Coutinho, S. G.
author_role author
author2 Bertho, A. L.
Oliveira Neto, M. P.
Coutinho, S. G.
author2_role author
author
author
dc.contributor.author.fl_str_mv Cruz, A. M.
Bertho, A. L.
Oliveira Neto, M. P.
Coutinho, S. G.
dc.subject.en.en_US.fl_str_mv Flow cytometry
Gamma-delta
Interferon-gammaR
Interleukin-2R
Leishmaniasis lesions
T lymphocytes
topic Flow cytometry
Gamma-delta
Interferon-gammaR
Interleukin-2R
Leishmaniasis lesions
T lymphocytes
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunopatologia. Rio de Janeiro, RJ, Brasil.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2023-02-06T19:30:01Z
dc.date.available.fl_str_mv 2023-02-06T19:30:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CRUZ, A. M. et al. Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions. British Journal of Dermatology, Grã-Bretanha, v. 153, p. 537-543, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56899
dc.identifier.issn.en_US.fl_str_mv 0366-077X
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-2133.2005.06647.x
identifier_str_mv CRUZ, A. M. et al. Flow cytometric analysis of cellular infiltrate from American tegumentary leishmaniasis lesions. British Journal of Dermatology, Grã-Bretanha, v. 153, p. 537-543, 2005.
0366-077X
10.1111/j.1365-2133.2005.06647.x
url https://www.arca.fiocruz.br/handle/icict/56899
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv British Association of Dermatologists
publisher.none.fl_str_mv British Association of Dermatologists
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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