Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23933 |
Resumo: | Vídeo Relacionado ao artigo: https://www.youtube.com/watch?v=Tpc3rW9zsb8 |
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Cumberworth, Stephanie L.Barrie, Jennifer A.Cunningham, Madeleine E.Figueiredo, Daniely Paulino Gomes deSchultz, VerenaWilder-Smith, Adrian J.Brennan, BenjaminPena, Lindomar J.França, Rafael Freitas de OliveiraLinington, ChristopherBarnett, Susan C.Willison, Hugh J.Kohl, AlainEdgar, Julia M.2018-01-17T12:44:37Z2018-01-17T12:44:37Z2017CUMBERWORTH, S. L. et al. Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected. Acta Neuropathologica Communications, v. 5, n. 1, p. 1-16, Dec. 2017.2051-5960https://www.arca.fiocruz.br/handle/icict/2393310.1186/s40478-017-0450-8Vídeo Relacionado ao artigo: https://www.youtube.com/watch?v=Tpc3rW9zsb8Este estudo foi financiado pelo UK Medical Research Council [MC_UU_12014, MR / N017552 / 1 (AK); Resposta rápida ZIKA MC_PC_15105 (HW, JME, AK); MR / K501335 / 1 (Bolsa de Treinamento de Doutorado, SLC)], Centro Nacional de Reposição, Redução e Refinamento (NC / L000423 / 1, JME, CL) e Wellcome Trust (203680 / Z / 16 / Z, WT092805; HJW). Este projecto foi parcialmente financiado através do programa de investigação e inovação Horizonte 2020 da União Europeia no âmbito do acordo de subvenção ZikaPLAN n.º 734584 (HJW, JME, SB, CL).MRC-University of Glasgow. Centre for Virus Research. G61 1QH. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.MRC-University of Glasgow. Centre for Virus Research. G61 1QH. Glasgow, Scotland, UK.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Virologia. Recife, PE, Brasil.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK.MRC-University of Glasgow. Centre for Virus Research. G61 1QH. Glasgow, Scotland, UK.University of Glasgow. College of Medical Veterinary and Life Sciences. Institute of Infection, Immunity and Inflammation. G12 8TA. Glasgow, Scotland, UK / Max Planck Institute for Experimental Medicine. Department of Neurogenetics. Goettingen, Germany.The recent global outbreak of Zika virus (ZIKV) infection has been linked to severe neurological disorders affecting the peripheral and central nervous systems (PNS and CNS, respectively). The pathobiology underlying these diverse clinical phenotypes are the subject of intense research; however, even the principal neural cell types vulnerable to productive Zika infection remain poorly characterised. Here we used CNS and PNS myelinating cultures from wild type and Ifnar1 knockout mice to examine neuronal and glial tropism and short-term consequences of direct infection with a Brazilian variant of ZIKV. Cell cultures were infected pre- or post-myelination for various intervals, then stained with cell-type and ZIKV-specific antibodies. In bypassing systemic immunity using ex vivo culture, and the type I interferon response in Ifnar1 deficient cells, we were able to evaluate the intrinsic infectivity of neural cells. Through systematic quantification of ZIKV infected cells in myelinating cultures, we found that ZIKV infection is enhanced in the absence of the type I interferon responses and that CNS cells are considerably more susceptible to infection than PNS cells. In particular, we demonstrate that CNS axons and myelinating oligodendrocytes are especially vulnerable to injury. These results have implications for understanding the pathobiology of neurological symptoms associated with ZIKV infection. Furthermore, we provide a quantifiable ex vivo infection model that can be used for fundamental and therapeutic studies on viral neuroinvasion and its consequences.engAxonSistema nervoso centralMurineMielinaSistema nervoso periféricoTropismoVírus ZikaAxonCentral nervous systemMurineMyelinPeripheral nervous systemTropismZika virusZika virusSistema NervosoZika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affectedinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23933/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALIAM - Zika virus tropism and interactions in myelinating neural cell cultures ....pdfIAM - Zika virus tropism and interactions in myelinating neural cell cultures ....pdfapplication/pdf10397042https://www.arca.fiocruz.br/bitstream/icict/23933/2/IAM%20-%20Zika%20virus%20tropism%20and%20interactions%20in%20myelinating%20neural%20cell%20cultures%20....pdf2624113b850489dd1f803ee5fd981bcaMD52TEXTIAM - 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| dc.title.pt_BR.fl_str_mv |
Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected |
| title |
Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected |
| spellingShingle |
Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected Cumberworth, Stephanie L. Axon Sistema nervoso central Murine Mielina Sistema nervoso periférico Tropismo Vírus Zika Axon Central nervous system Murine Myelin Peripheral nervous system Tropism Zika virus Zika virus Sistema Nervoso |
| title_short |
Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected |
| title_full |
Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected |
| title_fullStr |
Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected |
| title_full_unstemmed |
Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected |
| title_sort |
Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected |
| author |
Cumberworth, Stephanie L. |
| author_facet |
Cumberworth, Stephanie L. Barrie, Jennifer A. Cunningham, Madeleine E. Figueiredo, Daniely Paulino Gomes de Schultz, Verena Wilder-Smith, Adrian J. Brennan, Benjamin Pena, Lindomar J. França, Rafael Freitas de Oliveira Linington, Christopher Barnett, Susan C. Willison, Hugh J. Kohl, Alain Edgar, Julia M. |
| author_role |
author |
| author2 |
Barrie, Jennifer A. Cunningham, Madeleine E. Figueiredo, Daniely Paulino Gomes de Schultz, Verena Wilder-Smith, Adrian J. Brennan, Benjamin Pena, Lindomar J. França, Rafael Freitas de Oliveira Linington, Christopher Barnett, Susan C. Willison, Hugh J. Kohl, Alain Edgar, Julia M. |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cumberworth, Stephanie L. Barrie, Jennifer A. Cunningham, Madeleine E. Figueiredo, Daniely Paulino Gomes de Schultz, Verena Wilder-Smith, Adrian J. Brennan, Benjamin Pena, Lindomar J. França, Rafael Freitas de Oliveira Linington, Christopher Barnett, Susan C. Willison, Hugh J. Kohl, Alain Edgar, Julia M. |
| dc.subject.other.pt_BR.fl_str_mv |
Axon Sistema nervoso central Murine Mielina Sistema nervoso periférico Tropismo Vírus Zika |
| topic |
Axon Sistema nervoso central Murine Mielina Sistema nervoso periférico Tropismo Vírus Zika Axon Central nervous system Murine Myelin Peripheral nervous system Tropism Zika virus Zika virus Sistema Nervoso |
| dc.subject.en.pt_BR.fl_str_mv |
Axon Central nervous system Murine Myelin Peripheral nervous system Tropism Zika virus |
| dc.subject.decs.pt_BR.fl_str_mv |
Zika virus Sistema Nervoso |
| description |
Vídeo Relacionado ao artigo: https://www.youtube.com/watch?v=Tpc3rW9zsb8 |
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2017 |
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2017 |
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2018-01-17T12:44:37Z |
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2018-01-17T12:44:37Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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CUMBERWORTH, S. L. et al. Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected. Acta Neuropathologica Communications, v. 5, n. 1, p. 1-16, Dec. 2017. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/23933 |
| dc.identifier.issn.pt_BR.fl_str_mv |
2051-5960 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s40478-017-0450-8 |
| identifier_str_mv |
CUMBERWORTH, S. L. et al. Zika virus tropism and interactions in myelinating neural cell cultures: CNS cells and myelin are preferentially affected. Acta Neuropathologica Communications, v. 5, n. 1, p. 1-16, Dec. 2017. 2051-5960 10.1186/s40478-017-0450-8 |
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