Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom

Detalhes bibliográficos
Autor(a) principal: Barral Netto, Manoel
Data de Publicação: 1990
Outros Autores: Schriefer, Albert, Vinhas, Vera, Almeida, A. R
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26349
Resumo: Manoel, Barral Netto. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Barral Netto, ManoelSchriefer, AlbertVinhas, VeraAlmeida, A. R2018-05-09T19:14:21Z2018-05-09T19:14:21Z1990BARRAL NETTO, M. et al. Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom. Toxicon, v . 28, n. 9, p. 1053-1061, 1990.0041-0101https://www.arca.fiocruz.br/handle/icict/26349Manoel, Barral Netto. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.CNPq's (Conselho National de Desenvolvimento Cientifico e Tecnologico) scientific initiation fellowship . This project was supported by Financiadora de Estudos e Projetos (FINEP).Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilEnzyme-linked immunosorbent assay for the detection of Bothrops jararaca venom. Toxicon 28, 1053-1061, 1990.-This study reports an enzyme-linked immunosorbent assay for detecting Bothrops jararaca venom in fluids, employing the sandwich method with biotin/avidin amplification. The assay exhibits high accuracy in correlating optical densities with venom concentrations (r = 0.98), high reproducibility, low background and limited crossreactivity with venom from other snake genera . Nevertheless, it was unable to distinguish among venoms from different bothropic species. Using this method we evaluated the serum kinetics of Bothrops jararaca venom in C57BL/6 mice. High concentrations were found in serum just 15 min after injection (151 f41 ng/ml; mean±S.D.), followed by a progressive fall (102 f46, 74 f39 and 50±22ng/ml after 1, 3 and 6 hr respectively), being undetectable by 24 hr. Such serum kinetics indicates a pattern of a rapid absorption of venom from the inoculation site, followed by a slow and progressive drop in its serum levels . This ELISA was a reliable tool in the determination of Bothrops jararaca venom levels in mouse serum, and may become useful in other fields of bothropic venom researchengElsevierBothrops jararacaVenenoVenenos de CascavelEnsaio imunoenzimáticoSoroBothrops jararacaVenomJararaca venomImmunosorbent assaySerumEnzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venominfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26349/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBarral MN Enzyme-linked immunosorbent assay .....pdfBarral MN Enzyme-linked immunosorbent assay 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dc.title.pt_BR.fl_str_mv Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom
title Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom
spellingShingle Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom
Barral Netto, Manoel
Bothrops jararaca
Veneno
Venenos de Cascavel
Ensaio imunoenzimático
Soro
Bothrops jararaca
Venom
Jararaca venom
Immunosorbent assay
Serum
title_short Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom
title_full Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom
title_fullStr Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom
title_full_unstemmed Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom
title_sort Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom
author Barral Netto, Manoel
author_facet Barral Netto, Manoel
Schriefer, Albert
Vinhas, Vera
Almeida, A. R
author_role author
author2 Schriefer, Albert
Vinhas, Vera
Almeida, A. R
author2_role author
author
author
dc.contributor.author.fl_str_mv Barral Netto, Manoel
Schriefer, Albert
Vinhas, Vera
Almeida, A. R
dc.subject.other.pt_BR.fl_str_mv Bothrops jararaca
Veneno
Venenos de Cascavel
Ensaio imunoenzimático
Soro
topic Bothrops jararaca
Veneno
Venenos de Cascavel
Ensaio imunoenzimático
Soro
Bothrops jararaca
Venom
Jararaca venom
Immunosorbent assay
Serum
dc.subject.en.pt_BR.fl_str_mv Bothrops jararaca
Venom
Jararaca venom
Immunosorbent assay
Serum
description Manoel, Barral Netto. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 1990
dc.date.issued.fl_str_mv 1990
dc.date.accessioned.fl_str_mv 2018-05-09T19:14:21Z
dc.date.available.fl_str_mv 2018-05-09T19:14:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARRAL NETTO, M. et al. Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom. Toxicon, v . 28, n. 9, p. 1053-1061, 1990.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26349
dc.identifier.issn.pt_BR.fl_str_mv 0041-0101
identifier_str_mv BARRAL NETTO, M. et al. Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom. Toxicon, v . 28, n. 9, p. 1053-1061, 1990.
0041-0101
url https://www.arca.fiocruz.br/handle/icict/26349
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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