Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated

Detalhes bibliográficos
Autor(a) principal: Bailão, Elisa Flávia Luiz Cardoso
Data de Publicação: 2014
Outros Autores: Parente, Juliana Alves, Pigosso, Laurine Lacerda, de Castro, Kelly Pacheco, Fonseca, Fernanda Lopes, Silva-Bailão, Mirelle Garcia, Báo, Sônia Nair, Bailão, Alexandre Melo, Rodrigues, Marcio Lourenço, Hernandez, Orville, McEwen, Juan G., Soares, Célia Maria de Almeida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31989
Resumo: Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil / Universidade Estadual de Goiás. Unidade Universitária de Iporá. Iporá, GO, Brasil / Universidade de Brasília. Faculdade de Medicina. Programa de Pós Graduação em Patologia Molecular. Brasília, DF, Brasil.
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spelling Bailão, Elisa Flávia Luiz CardosoParente, Juliana AlvesPigosso, Laurine Lacerdade Castro, Kelly PachecoFonseca, Fernanda LopesSilva-Bailão, Mirelle GarciaBáo, Sônia NairBailão, Alexandre MeloRodrigues, Marcio LourençoHernandez, OrvilleMcEwen, Juan G.Soares, Célia Maria de Almeida2019-03-07T18:46:49Z2019-03-07T18:46:49Z2014BAILÃO, Elisa Flávia Luiz Cardoso et al. Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated. PLoS Neglected Tropical Disease, v. 8, n. 5, p. 1-20, 2014.1935-2735https://www.arca.fiocruz.br/handle/icict/3198910.1371/journal.pntd.0002856engPLOSHemoglobin uptake by Paracoccidioides spp. is receptor-mediatedinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil / Universidade Estadual de Goiás. Unidade Universitária de Iporá. Iporá, GO, Brasil / Universidade de Brasília. Faculdade de Medicina. Programa de Pós Graduação em Patologia Molecular. Brasília, DF, Brasil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil / Universidade de Brasília. Faculdade de Medicina. Programa de Pós Graduação em Patologia Molecular. Brasília, DF, Brasil.Universidade de Brasília. Laboratório de Microscopia Eletrônica. Brasilia, DF, Brasil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Corporación para Investigaciones Biológicas. Unidad de Biología Celular y Molecular. Medellín, Colombia / Institución Universitaria Colegio Mayor de Antioquia. Facultad de Ciencias de la Salud. Medellín, Colombia.Corporación para Investigaciones Biológicas (CIB). Unidad de Biología Celular y Molecular. Medellín, Colombia / Universidad de Antioquia. Facultad de Medicina. Medellín, Colombia.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.Iron is essential for the proliferation of fungal pathogens during infection. The availability of iron is limited due to its association with host proteins. Fungal pathogens have evolved different mechanisms to acquire iron from host; however, little is known regarding how Paracoccidioides species incorporate and metabolize this ion. In this work, host iron sources that are used by Paracoccidioides spp. were investigated. Robust fungal growth in the presence of the iron-containing molecules hemin and hemoglobin was observed. Paracoccidioides spp. present hemolytic activity and have the ability to internalize a protoporphyrin ring. Using real-time PCR and nanoUPLC-MSE proteomic approaches, fungal growth in the presence of hemoglobin was shown to result in the positive regulation of transcripts that encode putative hemoglobin receptors, in addition to the induction of proteins that are required for amino acid metabolism and vacuolar protein degradation. In fact, one hemoglobin receptor ortholog, Rbt5, was identified as a surface GPI-anchored protein that recognized hemin, protoporphyrin and hemoglobin in vitro. Antisense RNA technology and Agrobacterium tumefaciens-mediated transformation were used to generate mitotically stable Pbrbt5 mutants. The knockdown strain had a lower survival inside macrophages and in mouse spleen when compared with the parental strain, which suggested that Rbt5 could act as a virulence factor. In summary, our data indicate that Paracoccidioides spp. can use hemoglobin as an iron source most likely through receptor-mediated pathways that might be relevant for pathogenic mechanisms.Hemoglobin UptakeParacoccidioides spp.Receptor-Mediatedinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83101https://www.arca.fiocruz.br/bitstream/icict/31989/1/license.txt425ef259bcb460998407fbfed7c102d0MD51ORIGINALve_Marcio_Rodrigues_etal_CDTS_2014c.pdfve_Marcio_Rodrigues_etal_CDTS_2014c.pdfapplication/pdf5468839https://www.arca.fiocruz.br/bitstream/icict/31989/2/ve_Marcio_Rodrigues_etal_CDTS_2014c.pdf2b9bf1ba4f0c431b6369cf260cb24067MD52TEXTve_Marcio_Rodrigues_etal_CDTS_2014c.pdf.txtve_Marcio_Rodrigues_etal_CDTS_2014c.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
title Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
spellingShingle Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
Bailão, Elisa Flávia Luiz Cardoso
Hemoglobin Uptake
Paracoccidioides spp.
Receptor-Mediated
title_short Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
title_full Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
title_fullStr Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
title_full_unstemmed Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
title_sort Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated
author Bailão, Elisa Flávia Luiz Cardoso
author_facet Bailão, Elisa Flávia Luiz Cardoso
Parente, Juliana Alves
Pigosso, Laurine Lacerda
de Castro, Kelly Pacheco
Fonseca, Fernanda Lopes
Silva-Bailão, Mirelle Garcia
Báo, Sônia Nair
Bailão, Alexandre Melo
Rodrigues, Marcio Lourenço
Hernandez, Orville
McEwen, Juan G.
Soares, Célia Maria de Almeida
author_role author
author2 Parente, Juliana Alves
Pigosso, Laurine Lacerda
de Castro, Kelly Pacheco
Fonseca, Fernanda Lopes
Silva-Bailão, Mirelle Garcia
Báo, Sônia Nair
Bailão, Alexandre Melo
Rodrigues, Marcio Lourenço
Hernandez, Orville
McEwen, Juan G.
Soares, Célia Maria de Almeida
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bailão, Elisa Flávia Luiz Cardoso
Parente, Juliana Alves
Pigosso, Laurine Lacerda
de Castro, Kelly Pacheco
Fonseca, Fernanda Lopes
Silva-Bailão, Mirelle Garcia
Báo, Sônia Nair
Bailão, Alexandre Melo
Rodrigues, Marcio Lourenço
Hernandez, Orville
McEwen, Juan G.
Soares, Célia Maria de Almeida
dc.subject.en.pt_BR.fl_str_mv Hemoglobin Uptake
Paracoccidioides spp.
Receptor-Mediated
topic Hemoglobin Uptake
Paracoccidioides spp.
Receptor-Mediated
description Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil / Universidade Estadual de Goiás. Unidade Universitária de Iporá. Iporá, GO, Brasil / Universidade de Brasília. Faculdade de Medicina. Programa de Pós Graduação em Patologia Molecular. Brasília, DF, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2019-03-07T18:46:49Z
dc.date.available.fl_str_mv 2019-03-07T18:46:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BAILÃO, Elisa Flávia Luiz Cardoso et al. Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated. PLoS Neglected Tropical Disease, v. 8, n. 5, p. 1-20, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31989
dc.identifier.issn.pt_BR.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0002856
identifier_str_mv BAILÃO, Elisa Flávia Luiz Cardoso et al. Hemoglobin uptake by Paracoccidioides spp. is receptor-mediated. PLoS Neglected Tropical Disease, v. 8, n. 5, p. 1-20, 2014.
1935-2735
10.1371/journal.pntd.0002856
url https://www.arca.fiocruz.br/handle/icict/31989
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/31989/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/31989/2/ve_Marcio_Rodrigues_etal_CDTS_2014c.pdf
https://www.arca.fiocruz.br/bitstream/icict/31989/3/ve_Marcio_Rodrigues_etal_CDTS_2014c.pdf.txt
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