Presence of leptospires on genital tract of mares with reproductive problems

Detalhes bibliográficos
Autor(a) principal: Hamond, Camila
Data de Publicação: 2015
Outros Autores: Pestana, Cristiane P., Souza, Cláudio Marcos Rocha de, Cunha, Luis Eduardo R., Brandão, Felipe Z., Medeiros, Marco Alberto, Lilenbaum, Walter
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1016/j.vetmic.2015.06.014
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32007
Resumo: Artigo em acesso aberto no link da Editora.
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spelling Hamond, CamilaPestana, Cristiane P.Souza, Cláudio Marcos Rocha deCunha, Luis Eduardo R.Brandão, Felipe Z.Medeiros, Marco AlbertoLilenbaum, Walter2019-03-08T18:39:50Z2019-03-08T18:39:50Z2015HAMOND, Camila et al. Presence of leptospires on genital tract of mares with reproductive problems. Veterinary Microbiology, Amsterdam, v. 179, n. 3-4, p. 264-269, 2015.1873-2542https://www.arca.fiocruz.br/handle/icict/3200710.1016/j.vetmic.2015.06.014Artigo em acesso aberto no link da Editora.Universidade Federal Fluminense. Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Instituto Vital Brasil. Niterói, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Medicina Veterinária. Departamento de Patologia e Veterinária Clínica, Faculdade de Veterinária. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense.Departamento de Microbiologia e Parasitologia. Laboratório de Bacteriologia Veterinária. Niterói, RJ, Brasil.Leptospirosis is a zoonotic disease of global importance, and has a worldwide distribution. Equine leptospirosis is commonly manifested by recurrent uveitis, reproductive disorders, as abortions, embryonic absorption, stillbirth and the birth of weak foals. The aim of this study was to verify the presence of Leptospira sp or its DNA in genital tract of mares with reproductive problems. A total of 38 mares with reproductive problems were studied. All the mares were sampled for blood (for serology), urine (for culturing and qPCR), vaginal fluid-VF and endometrial biopsy-EB (for culturing, qPCR and indirect immunofluorescence). PCRs products were sequenced for secY gene. Seventeen (44.7%) serum samples were reactive, predominantly against serogroups Australis (76.4%) and Pomona (23.6%). No positive culture was obtained, but DNA was detected by qPCR on urine samples (26.3%), VF (44.7%) and EB (18.4%) collected 2 months or longer following diagnosis of early fetal death and endometritis. Leptospira cell aggregations were visible by indirect immunofluorescence on 57.1% (4/7) EBs and 17.6% (3/17) VFs. A total of 18 amplicons showed interpretable sequences. Out of those 18 amplicons, 15 presented 100% of identity with the species L. interrogans (sv Bratislava and Pomona), while three were L. borgpertersenii. This study suggests the presence of leptospires in the uterus of mares with reproductive problems. Moreover, serology was shown not to be indicated for the diagnosis of presumptive Leptospira infection in early gestation. The most common agent of the genital infection in those mares was L. interrogans, most probably sg Australis.engPresence of leptospires on genital tract of mares with reproductive problemsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHorseLeptospiraUterusinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/32007/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINAL1-s2.0-S0378113515002503-main.pdf1-s2.0-S0378113515002503-main.pdfapplication/pdf614712https://www.arca.fiocruz.br/bitstream/icict/32007/2/1-s2.0-S0378113515002503-main.pdfb67581c88e6e2b61d97b00c6012a764bMD52TEXT1-s2.0-S0378113515002503-main.pdf.txt1-s2.0-S0378113515002503-main.pdf.txtExtracted texttext/plain33400https://www.arca.fiocruz.br/bitstream/icict/32007/3/1-s2.0-S0378113515002503-main.pdf.txt9111f54f47c875a239d853c864ea28f6MD53icict/320072019-04-04 02:05:01.876oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-04-04T05:05:01Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Presence of leptospires on genital tract of mares with reproductive problems
title Presence of leptospires on genital tract of mares with reproductive problems
spellingShingle Presence of leptospires on genital tract of mares with reproductive problems
Hamond, Camila
Horse
Leptospira
Uterus
title_short Presence of leptospires on genital tract of mares with reproductive problems
title_full Presence of leptospires on genital tract of mares with reproductive problems
title_fullStr Presence of leptospires on genital tract of mares with reproductive problems
title_full_unstemmed Presence of leptospires on genital tract of mares with reproductive problems
title_sort Presence of leptospires on genital tract of mares with reproductive problems
author Hamond, Camila
author_facet Hamond, Camila
Pestana, Cristiane P.
Souza, Cláudio Marcos Rocha de
Cunha, Luis Eduardo R.
Brandão, Felipe Z.
Medeiros, Marco Alberto
Lilenbaum, Walter
author_role author
author2 Pestana, Cristiane P.
Souza, Cláudio Marcos Rocha de
Cunha, Luis Eduardo R.
Brandão, Felipe Z.
Medeiros, Marco Alberto
Lilenbaum, Walter
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Hamond, Camila
Pestana, Cristiane P.
Souza, Cláudio Marcos Rocha de
Cunha, Luis Eduardo R.
Brandão, Felipe Z.
Medeiros, Marco Alberto
Lilenbaum, Walter
dc.subject.en.pt_BR.fl_str_mv Horse
Leptospira
Uterus
topic Horse
Leptospira
Uterus
description Artigo em acesso aberto no link da Editora.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2019-03-08T18:39:50Z
dc.date.available.fl_str_mv 2019-03-08T18:39:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv HAMOND, Camila et al. Presence of leptospires on genital tract of mares with reproductive problems. Veterinary Microbiology, Amsterdam, v. 179, n. 3-4, p. 264-269, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32007
dc.identifier.issn.pt_BR.fl_str_mv 1873-2542
dc.identifier.doi.none.fl_str_mv 10.1016/j.vetmic.2015.06.014
identifier_str_mv HAMOND, Camila et al. Presence of leptospires on genital tract of mares with reproductive problems. Veterinary Microbiology, Amsterdam, v. 179, n. 3-4, p. 264-269, 2015.
1873-2542
10.1016/j.vetmic.2015.06.014
url https://www.arca.fiocruz.br/handle/icict/32007
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