Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10209 |
Resumo: | Universidade do Estado do Rio de Janeiro (UERJ). Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. |
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Freitas, Flávia A. D. deBernardo, VagnerGomgnimbou, Michel KSola, ChristopheSiqueira, Hélio RPereira, Márcia A. SFandinho, Fátima C. OGomes, Harrison MAraújo, Marcelo E. ISuffys, Philip NoelMarques, Elizabeth AAlbano, Rodolpho Mattos2015-05-04T17:07:30Z2015-05-04T17:07:30Z2014FREITAS. Flávia A. D. de et al. Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil. Plos One, v.9, n.8, 11p, 2014.https://www.arca.fiocruz.br/handle/icict/1020910.1371/journal.pone.0104100engPlos OneTuberculoseBrasilMycobacterium tuberculosisBrazilReference CenterTuberculosisMultidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro (UERJ). Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ). Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.CNRS–Université Paris–Sud, Institut de Génétique et Microbiologie– Infection Genetics Emerging Pathogens Evolution Team. Orsay, France.CNRS–Université Paris–Sud, Institut de Génétique et Microbiologie– Infection Genetics Emerging Pathogens Evolution Team. Orsay, France.Universidade do Estado do Rio de Janeiro (UERJ). Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Referência Professor Hélio Fraga. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ). Faculdade de Ciências Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ). Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Background: Multidrug resistance is a critical factor in tuberculosis control. To gain better understanding of multidrug resistant tuberculosis in Brazil, a retrospective study was performed to compare genotypic diversity and drug resistance associated mutations in Mycobacterium tuberculosis isolates from a national reference center. Methods and Findings: Ninety-nine multidrug resistant isolates from 12 Brazilian states were studied. Drug-resistance patterns were determined and the rpoB and katG genes were screened for mutations. Genotypic diversity was investigated by IS6110-RFLP and Luminex 47 spoligotyping. Mutations in rpoB and katG were seen in 91% and 93% of the isolates, respectively. Codon 315 katG mutations occurred in 82.8% of the isolates with a predominance of the Ser315Thr substitution. Twenty-five isolates were clustered in 11 groups with identical IS6110-RFLP patterns while 74 showed unique patterns with no association between mutation frequencies or susceptibility profiles. The most prevalent spoligotyping lineages were LAM (47%), T (17%) and Haarlen (12%). The Haarlen lineage showed a higher frequency of codon 516 rpoB mutations while codon 531 mutations prevailed in the other isolates. Conclusions: Our data suggest that there were no major multidrug resistant M. tuberculosis strains transmitted among patients referred to the reference center, indicating an independent acquisition of resistance. In addition, drug resistance associated mutation profiles were well established among the main spoligotyping lineages found in these Brazilian multidrug resistant isolates, providing useful data for patient management and treatment.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10209/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALharrison_gomesetal_IOC_@014.pdfapplication/pdf1071521https://www.arca.fiocruz.br/bitstream/icict/10209/2/harrison_gomesetal_IOC_%40014.pdf010d362ce512960216fbfc2738013912MD52TEXTharrison_gomesetal_IOC_@014.pdf.txtharrison_gomesetal_IOC_@014.pdf.txtExtracted texttext/plain55239https://www.arca.fiocruz.br/bitstream/icict/10209/3/harrison_gomesetal_IOC_%40014.pdf.txt5ad331d560a9656ea581a7d70e4f998eMD53icict/102092022-06-24 13:03:05.634oai:www.arca.fiocruz.br:icict/10209TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:03:05Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil |
| title |
Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil |
| spellingShingle |
Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil Freitas, Flávia A. D. de Tuberculose Brasil Mycobacterium tuberculosis Brazil Reference Center Tuberculosis |
| title_short |
Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil |
| title_full |
Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil |
| title_fullStr |
Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil |
| title_full_unstemmed |
Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil |
| title_sort |
Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil |
| author |
Freitas, Flávia A. D. de |
| author_facet |
Freitas, Flávia A. D. de Bernardo, Vagner Gomgnimbou, Michel K Sola, Christophe Siqueira, Hélio R Pereira, Márcia A. S Fandinho, Fátima C. O Gomes, Harrison M Araújo, Marcelo E. I Suffys, Philip Noel Marques, Elizabeth A Albano, Rodolpho Mattos |
| author_role |
author |
| author2 |
Bernardo, Vagner Gomgnimbou, Michel K Sola, Christophe Siqueira, Hélio R Pereira, Márcia A. S Fandinho, Fátima C. O Gomes, Harrison M Araújo, Marcelo E. I Suffys, Philip Noel Marques, Elizabeth A Albano, Rodolpho Mattos |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Freitas, Flávia A. D. de Bernardo, Vagner Gomgnimbou, Michel K Sola, Christophe Siqueira, Hélio R Pereira, Márcia A. S Fandinho, Fátima C. O Gomes, Harrison M Araújo, Marcelo E. I Suffys, Philip Noel Marques, Elizabeth A Albano, Rodolpho Mattos |
| dc.subject.other.pt_BR.fl_str_mv |
Tuberculose Brasil |
| topic |
Tuberculose Brasil Mycobacterium tuberculosis Brazil Reference Center Tuberculosis |
| dc.subject.en.pt_BR.fl_str_mv |
Mycobacterium tuberculosis Brazil Reference Center |
| dc.subject.es.pt_BR.fl_str_mv |
Tuberculosis |
| description |
Universidade do Estado do Rio de Janeiro (UERJ). Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. |
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2014 |
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2014 |
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2015-05-04T17:07:30Z |
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2015-05-04T17:07:30Z |
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FREITAS. Flávia A. D. de et al. Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil. Plos One, v.9, n.8, 11p, 2014. |
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https://www.arca.fiocruz.br/handle/icict/10209 |
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10.1371/journal.pone.0104100 |
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FREITAS. Flávia A. D. de et al. Multidrug Resistant Mycobacterium tuberculosis: A Retrospective katG and rpoB Mutation Profile Analysis in Isolates from a Reference Center in Brazil. Plos One, v.9, n.8, 11p, 2014. 10.1371/journal.pone.0104100 |
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