Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis

Detalhes bibliográficos
Autor(a) principal: Passos, Daniela Araújo Cunha
Data de Publicação: 2002
Outros Autores: Oliveira, Rosely Maria Zancopé, Felipe, M. Sueli S., Izacc, Silvia M., Deepe Jr, George S., Soares, Célia M. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33021
Resumo: 2019-11-09
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spelling Passos, Daniela Araújo CunhaOliveira, Rosely Maria ZancopéFelipe, M. Sueli S.Izacc, Silvia M.Deepe Jr, George S.Soares, Célia M. A.2019-05-09T14:52:21Z2019-05-09T14:52:21Z2002PASSOS, Daniela Araújo Cunha. Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis. Clinical and Diagnostic Laboratory Immunology, v. 9, n. 2, p. 374-377, Mar. 2002.1071-412Xhttps://www.arca.fiocruz.br/handle/icict/3302110.1128/cdli.9.2.374-377.20021098-6588engAmerican Society for MicrobiologyHeterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2019-11-09Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Hospital Evandro Chagas. Laboratório de Micologia Médica. Rio de Janeiro, RJ, Brasil.Universidade de Brasília. Instituto de Biologia. Laboratório de Biologia Molecular. Brasília, DF, Brasil.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.University of Cincinnati. College of Medicine. Division of Infectious Diseases. Cincinnati, OH, USA.Universidade Federal de Goiás. Instituto de Ciências Biológicas. Laboratório de Biologia Molecular. Goiânia, GO, Brasil.The complete coding cDNA of HSP60 from Paracoccidioides brasiliensis was overexpressed in an Escherichia coli host to produce high levels of recombinant protein. The protein was purified by affinity chromatography. A total of 169 human serum samples were tested for reactivity by Western blot analysis with the purified HSP60 recombinant protein. Immunoblots indicated that the recombinant P. brasiliensis HSP60 was recognized by antibodies in 72 of 75 sera from paracoccidioidomycosis patients. No cross-reactivity was detected with individual sera from patients with aspergillosis, sporotrichosis, cryptococcosis, and tuberculosis. Reactivity to HSP60 was observed in sera from 9.52% of control healthy individuals and 11.5% of patients with histoplasmosis. The high sensitivity and specificity (97.3 and 92.5%, respectively) for HSP60 suggested that the recombinant protein can be used singly or in association with other recombinant antigens to detect antibody responses in P. brasiliensis-infected patients.Paracoccidioides brasiliensisParacoccidioidomycosisHSP60 recombinant proteininfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33021/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Cunha_Daniela_etal_INI_2002.pdfve_Cunha_Daniela_etal_INI_2002.pdfapplication/pdf531310https://www.arca.fiocruz.br/bitstream/icict/33021/2/ve_Cunha_Daniela_etal_INI_2002.pdf1cf8a8eeb6760c6f70925d0fbcab886cMD52TEXTve_Cunha_Daniela_etal_INI_2002.pdf.txtve_Cunha_Daniela_etal_INI_2002.pdf.txtExtracted texttext/plain23400https://www.arca.fiocruz.br/bitstream/icict/33021/3/ve_Cunha_Daniela_etal_INI_2002.pdf.txtad7371b642354963f06ec0bc04afcd36MD53icict/330212021-05-18 13:33:09.564oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-05-18T16:33:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis
title Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis
spellingShingle Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis
Passos, Daniela Araújo Cunha
Paracoccidioides brasiliensis
Paracoccidioidomycosis
HSP60 recombinant protein
title_short Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis
title_full Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis
title_fullStr Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis
title_full_unstemmed Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis
title_sort Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis
author Passos, Daniela Araújo Cunha
author_facet Passos, Daniela Araújo Cunha
Oliveira, Rosely Maria Zancopé
Felipe, M. Sueli S.
Izacc, Silvia M.
Deepe Jr, George S.
Soares, Célia M. A.
author_role author
author2 Oliveira, Rosely Maria Zancopé
Felipe, M. Sueli S.
Izacc, Silvia M.
Deepe Jr, George S.
Soares, Célia M. A.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Passos, Daniela Araújo Cunha
Oliveira, Rosely Maria Zancopé
Felipe, M. Sueli S.
Izacc, Silvia M.
Deepe Jr, George S.
Soares, Célia M. A.
dc.subject.en.pt_BR.fl_str_mv Paracoccidioides brasiliensis
Paracoccidioidomycosis
HSP60 recombinant protein
topic Paracoccidioides brasiliensis
Paracoccidioidomycosis
HSP60 recombinant protein
description 2019-11-09
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2019-05-09T14:52:21Z
dc.date.available.fl_str_mv 2019-05-09T14:52:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PASSOS, Daniela Araújo Cunha. Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis. Clinical and Diagnostic Laboratory Immunology, v. 9, n. 2, p. 374-377, Mar. 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33021
dc.identifier.issn.pt_BR.fl_str_mv 1071-412X
dc.identifier.doi.pt_BR.fl_str_mv 10.1128/cdli.9.2.374-377.2002
dc.identifier.eissn.none.fl_str_mv 1098-6588
identifier_str_mv PASSOS, Daniela Araújo Cunha. Heterologous expression, purification, and immunological reactivity of a recombinant HSP60 from Paracoccidioides brasiliensis. Clinical and Diagnostic Laboratory Immunology, v. 9, n. 2, p. 374-377, Mar. 2002.
1071-412X
10.1128/cdli.9.2.374-377.2002
1098-6588
url https://www.arca.fiocruz.br/handle/icict/33021
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/33021/2/ve_Cunha_Daniela_etal_INI_2002.pdf
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