Characterization of α-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars

Detalhes bibliográficos
Autor(a) principal: Costa, Samara G. da
Data de Publicação: 2019
Outros Autores: Bates, Paul, Dillon, Rod, Genta, Fernando Ariel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35136
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.
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spelling Costa, Samara G. daBates, PaulDillon, RodGenta, Fernando Ariel2019-08-27T17:41:34Z2019-08-27T17:41:34Z2019COSTA, Samara G. da et al. Characterization of a-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars. Frontiers in Physiology, v. 10, p. 1-15, Apr. 2019.1664-042Xhttps://www.arca.fiocruz.br/handle/icict/3513610.3389/fphys.2019.00248engFrontiers MediaLutzomyia longipalpisDigestãoAçúcarSacaraseLutzomyia longipalpisDigestionSugarSucrasea-glucosidaseGlucoside hydrolaseSacaraseCharacterization of α-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugarsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Lutzomyia longipalpis is the main vector of Leishmania infantum and exploits different food sources during development. Adults have a diet rich in sugars, and females also feed on blood. The sugar diet is essential for maintaining longevity, infection, and Leishmaniasis transmission. Carbohydrases, including α-glucosidases, are the main enzymes involved in the digestion of sugars. In this context, we studied the modulation of α-glucosidase activities in different feeding conditions and compartments of Lutzomyia longipalpis females, in order to characterize in detail their roles in the physiology of this insect. All tissues showed activity against MUαGlu and sucrose, with highest activities in the midgut and crop. Activity was 1,000 times higher on sucrose than on MUαGlu. Basal activities were observed in non-fed insects; blood feeding induced activity in the midgut contents, and sugar feeding modulated activity in midgut tissues. α-glucosidase activity changed after female exposure to different sugar concentrations or moieties. α-glucosidases from different tissues showed different biochemical properties, with an optimum pH around 7.0-8.0 and K M between 0.37 and 4.7 mM, when MUαGlu was used as substrate. Using sucrose as substrate, the optimum pH was around 6.0, and K M ranges between 11 and 800 mM. Enzymes from the crop and midgut tissues showed inhibition in high substrate concentrations (sucrose), with K I ranging from 39 to 400 mM, which explains the high K M values found. Chromatographic profiles confirmed that different α-glucosidases are been produced in L. longipalpis in different physiological contexts, with the distinction of at least four α-glucosidases. The results suggest that some of these enzymes are involved in different metabolic processes, like digestion of plant sugars, digestion of blood glycoproteins or glycolipids, and mobilization of energetic storages during starvation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35136/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFernandoGenta_SimoneCosta_etal_IOC_2019.pdfFernandoGenta_SimoneCosta_etal_IOC_2019.pdfapplication/pdf3097008https://www.arca.fiocruz.br/bitstream/icict/35136/2/FernandoGenta_SimoneCosta_etal_IOC_2019.pdfe8be22d078ecc4b57e4eb0f1632adcdaMD52TEXTFernandoGenta_SimoneCosta_etal_IOC_2019.pdf.txtFernandoGenta_SimoneCosta_etal_IOC_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Characterization of α-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars
title Characterization of α-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars
spellingShingle Characterization of α-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars
Costa, Samara G. da
Lutzomyia longipalpis
Digestão
Açúcar
Sacarase
Lutzomyia longipalpis
Digestion
Sugar
Sucrase
a-glucosidase
Glucoside hydrolase
Sacarase
title_short Characterization of α-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars
title_full Characterization of α-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars
title_fullStr Characterization of α-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars
title_full_unstemmed Characterization of α-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars
title_sort Characterization of α-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars
author Costa, Samara G. da
author_facet Costa, Samara G. da
Bates, Paul
Dillon, Rod
Genta, Fernando Ariel
author_role author
author2 Bates, Paul
Dillon, Rod
Genta, Fernando Ariel
author2_role author
author
author
dc.contributor.author.fl_str_mv Costa, Samara G. da
Bates, Paul
Dillon, Rod
Genta, Fernando Ariel
dc.subject.other.pt_BR.fl_str_mv Lutzomyia longipalpis
Digestão
Açúcar
Sacarase
topic Lutzomyia longipalpis
Digestão
Açúcar
Sacarase
Lutzomyia longipalpis
Digestion
Sugar
Sucrase
a-glucosidase
Glucoside hydrolase
Sacarase
dc.subject.en.pt_BR.fl_str_mv Lutzomyia longipalpis
Digestion
Sugar
Sucrase
a-glucosidase
Glucoside hydrolase
dc.subject.decs.pt_BR.fl_str_mv Sacarase
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-08-27T17:41:34Z
dc.date.available.fl_str_mv 2019-08-27T17:41:34Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Samara G. da et al. Characterization of a-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars. Frontiers in Physiology, v. 10, p. 1-15, Apr. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35136
dc.identifier.issn.pt_BR.fl_str_mv 1664-042X
dc.identifier.doi.none.fl_str_mv 10.3389/fphys.2019.00248
identifier_str_mv COSTA, Samara G. da et al. Characterization of a-Glucosidases From Lutzomyia longipalpis Reveals Independent Hydrolysis Systems for Plant or Blood Sugars. Frontiers in Physiology, v. 10, p. 1-15, Apr. 2019.
1664-042X
10.3389/fphys.2019.00248
url https://www.arca.fiocruz.br/handle/icict/35136
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/35136/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/35136/2/FernandoGenta_SimoneCosta_etal_IOC_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/35136/3/FernandoGenta_SimoneCosta_etal_IOC_2019.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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