SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays

Detalhes bibliográficos
Autor(a) principal: Sousa, Karoline Almeida Felix de
Data de Publicação: 2023
Outros Autores: Nonaka, Carolina Kymie Vasques, Mendonça, Renata Naves de Ávila, Mascarenhas, Verena Neiva, Weber, Thamires Gomes Lopes, Silva, Carlos Gustavo Regis, Mendes, Ana Verena Almeida, Khouri, Ricardo, Souza, Bruno Solano Freitas, Rocha, Clarissa Araújo Gurgel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58969
Resumo: Inova Fiocruz. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CNPq).
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spelling Sousa, Karoline Almeida Felix deNonaka, Carolina Kymie VasquesMendonça, Renata Naves de ÁvilaMascarenhas, Verena NeivaWeber, Thamires Gomes LopesSilva, Carlos Gustavo RegisMendes, Ana Verena AlmeidaKhouri, RicardoSouza, Bruno Solano FreitasRocha, Clarissa Araújo Gurgel2023-06-07T17:28:35Z2023-06-07T17:28:35Z2023SOUSA, Karoline Almeida Felix de et al. SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays. Diagnostics, v. 13, p. 1-8, 2023.2075-4418https://www.arca.fiocruz.br/handle/icict/5896910.3390/diagnostics13020329Inova Fiocruz. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CNPq).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto D'Or de Pesquisa e Ensino. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil.Instituto D'Or de Pesquisa e Ensino. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil.Hospital São Rafael. Salvador, BA, Brasil.Hospital São Rafael. Salvador, BA, Brasil.Hospital São Rafael. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Hospital São Rafael. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto D'Or de Pesquisa e Ensino. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Hospital São Rafael. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto D'Or de Pesquisa e Ensino. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Odontologia. Salvador, BA, Brasil.Background: Self-collected saliva samples can increase the diagnostic efficiency and benefit healthcare workers, patient care, and infection control. This study evaluated the performance of self-collected saliva samples compared to nasopharyngeal swabs using three commercial kits for the qualitative detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: Matched nasopharyngeal and saliva samples were collected from 103 patients with either asymptomatic or symptomatic COVID-19. Both samples were evaluated using three commercial kits (TaqCheck, Allplex, and TaqPath). To evaluate sample stability, viral RNA extraction was performed in the presence or absence of an RNA-stabilizing solution. Storage conditions, including the duration, temperature, and stability after freezing and thawing of the samples, were also evaluated. Results: All the saliva samples showed 100% concordance with the nasopharyngeal swab results using TaqCheck and Allplex kits, and 93% using TaqPath kit. No difference was observed in the samples that used the RNA-stabilizing solution compared to the group without the solution. The Ct values of the freeze–thawed samples after 30 days were higher than those on day 0; however, the results were consistent the fresh samples. Conclusion: The high concordance of SARS-CoV-2 detection via reverse transcription–polymerase chain reaction (RT-PCR) in matched saliva and nasopharyngeal samples using different commercial assays reinforces the concept that self-collected saliva samples are non-invasive, rapid, and reliable for diagnosing SARS-CoV-2 infection.engMDPICOVID 19SalivaMétodo diagnósticoSARS-CoV-2RT-PCRCOVID-19SalivaDiagnostic methodSARS-CoV-2RT-PCRCOVID 19SalivaMétodoDiagnósticoSARS-CoV-2Reação em Cadeia da Polimerase Via Transcriptase ReversaSARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assaysinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/58969/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDe Souza, K.A. Felix. SARS-CoV-2 Detection via RT-PCR....pdfDe Souza, K.A. Felix. SARS-CoV-2 Detection via RT-PCR....pdfapplication/pdf1044864https://www.arca.fiocruz.br/bitstream/icict/58969/2/De%20Souza%2c%20K.A.%20%20Felix.%20SARS-CoV-2%20Detection%20via%20RT-PCR....pdf2037680a5481fa41c96a98eda9b04746MD52icict/589692023-06-07 20:10:23.602oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-06-07T23:10:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays
title SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays
spellingShingle SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays
Sousa, Karoline Almeida Felix de
COVID 19
Saliva
Método diagnóstico
SARS-CoV-2
RT-PCR
COVID-19
Saliva
Diagnostic method
SARS-CoV-2
RT-PCR
COVID 19
Saliva
Método
Diagnóstico
SARS-CoV-2
Reação em Cadeia da Polimerase Via Transcriptase Reversa
title_short SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays
title_full SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays
title_fullStr SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays
title_full_unstemmed SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays
title_sort SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays
author Sousa, Karoline Almeida Felix de
author_facet Sousa, Karoline Almeida Felix de
Nonaka, Carolina Kymie Vasques
Mendonça, Renata Naves de Ávila
Mascarenhas, Verena Neiva
Weber, Thamires Gomes Lopes
Silva, Carlos Gustavo Regis
Mendes, Ana Verena Almeida
Khouri, Ricardo
Souza, Bruno Solano Freitas
Rocha, Clarissa Araújo Gurgel
author_role author
author2 Nonaka, Carolina Kymie Vasques
Mendonça, Renata Naves de Ávila
Mascarenhas, Verena Neiva
Weber, Thamires Gomes Lopes
Silva, Carlos Gustavo Regis
Mendes, Ana Verena Almeida
Khouri, Ricardo
Souza, Bruno Solano Freitas
Rocha, Clarissa Araújo Gurgel
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Karoline Almeida Felix de
Nonaka, Carolina Kymie Vasques
Mendonça, Renata Naves de Ávila
Mascarenhas, Verena Neiva
Weber, Thamires Gomes Lopes
Silva, Carlos Gustavo Regis
Mendes, Ana Verena Almeida
Khouri, Ricardo
Souza, Bruno Solano Freitas
Rocha, Clarissa Araújo Gurgel
dc.subject.other.en_US.fl_str_mv COVID 19
Saliva
Método diagnóstico
SARS-CoV-2
RT-PCR
topic COVID 19
Saliva
Método diagnóstico
SARS-CoV-2
RT-PCR
COVID-19
Saliva
Diagnostic method
SARS-CoV-2
RT-PCR
COVID 19
Saliva
Método
Diagnóstico
SARS-CoV-2
Reação em Cadeia da Polimerase Via Transcriptase Reversa
dc.subject.en.en_US.fl_str_mv COVID-19
Saliva
Diagnostic method
SARS-CoV-2
RT-PCR
dc.subject.decs.en_US.fl_str_mv COVID 19
Saliva
Método
Diagnóstico
SARS-CoV-2
Reação em Cadeia da Polimerase Via Transcriptase Reversa
description Inova Fiocruz. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CNPq).
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-06-07T17:28:35Z
dc.date.available.fl_str_mv 2023-06-07T17:28:35Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUSA, Karoline Almeida Felix de et al. SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays. Diagnostics, v. 13, p. 1-8, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58969
dc.identifier.issn.en_US.fl_str_mv 2075-4418
dc.identifier.doi.none.fl_str_mv 10.3390/diagnostics13020329
identifier_str_mv SOUSA, Karoline Almeida Felix de et al. SARS-CoV-2 detection via RT-PCR in matched saliva and nasopharyngeal samples reveals high concordance in different commercial assays. Diagnostics, v. 13, p. 1-8, 2023.
2075-4418
10.3390/diagnostics13020329
url https://www.arca.fiocruz.br/handle/icict/58969
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
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instname:Fundação Oswaldo Cruz (FIOCRUZ)
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bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/58969/1/license.txt
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