Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts

Zych, Jaiesa; Spangenberg, Lucia; Stimamiglio, Marco Augusto; Abud, Ana Paula Ressetti; Shigunov, Patrícia; Marchini, Fabrício Klerynton; Kuligovski, Crisciele; Cofré, Axel Helmut Rulf; Schittini, Andressa V.; Aguiar, Alessandra Melo de; Senegaglia, Alexandra Cristina; Brofman, Paulo Roberto Slud; Goldenberg, Samuel; Dallagiovanna, Bruno; Naya, Hugo; Dominguez, Alejandro Correa

Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts

Detalhes bibliográficos
Autor(a) principal:	Zych, Jaiesa
Data de Publicação:	2014
Outros Autores:	Spangenberg, Lucia, Stimamiglio, Marco Augusto, Abud, Ana Paula Ressetti, Shigunov, Patrícia, Marchini, Fabrício Klerynton, Kuligovski, Crisciele, Cofré, Axel Helmut Rulf, Schittini, Andressa V., Aguiar, Alessandra Melo de, Senegaglia, Alexandra Cristina, Brofman, Paulo Roberto Slud, Goldenberg, Samuel, Dallagiovanna, Bruno, Naya, Hugo, Dominguez, Alejandro Correa
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/9158
Resumo:	O artigo encontra-se disponível em acesso aberto no site do Editor.

Metadados do item

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network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Zych, JaiesaSpangenberg, LuciaStimamiglio, Marco AugustoAbud, Ana Paula RessettiShigunov, PatríciaMarchini, Fabrício KleryntonKuligovski, CriscieleCofré, Axel Helmut RulfSchittini, Andressa V.Aguiar, Alessandra Melo deSenegaglia, Alexandra CristinaBrofman, Paulo Roberto SludGoldenberg, SamuelDallagiovanna, BrunoNaya, HugoDominguez, Alejandro Correa2014-12-12T00:08:23Z2015-11-15T06:30:04Z2014ZYCH, Jaiesa et al. Polysome Profiling Shows the Identity of Human Adipose-Derived Stromal/Stem Cells in Detail and Clearly Distinguishes Them from Dermal Fibroblasts. Stem Cells and Development, v. 23, n. 22, p. 2791-2802, 2014.1547-3287https://www.arca.fiocruz.br/handle/icict/915810.1089/scd.2013.0496O artigo encontra-se disponível em acesso aberto no site do Editor.This work was supported by grants from Ministério da Saúde and Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq, FIOCRUZ-Pasteur Research Program, and Fundação Araucária. L.S. received fellowship from ANII (Agencia Nacional de Investigación e Innovación, Uruguay); S.G., J.Z., and B.D. from CNPq; P.S. from FIOCRUZ; and A.C. from Fundação Araucária.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Pasteur. Unidad de Bioinformática. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Pasteur. Unidad de Bioinformática. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Although fibroblasts and multipotent stromal/stem cells, including adipose-derived stromal cells (ADSCs), have been extensively studied, they cannot be clearly distinguished from each other. We, therefore, investigated the cellular and molecular characteristics of ADSCs and fibroblasts. ADSCs and fibroblasts share several morphological similarities and surface markers, but were clearly found to be different types of cells. Contrary to previous reports, fibroblasts were not able to differentiate into adipocytes, osteoblasts, or chondrocytes. Polysome-bound mRNA profiling revealed that*1,547 genes were differentially expressed (DE) in the two cell types; the genes were related to cell adhesion, the extracellular matrix, differentiation, and proliferation. These findings were confirmed by functional analyses showing that ADSCs had a greater adhesion capacity than fibroblasts; the proliferation rate of fibroblasts was also higher than that of ADSCs. Importantly, 185 DE genes were integral to the plasma membrane and, thus, candidate markers for ADSC isolation and manipulation. We also observed that an established marker of fibroblasts and ADSCs, CD105, was overexpressed in ADSCs at both mRNA and protein levels. CD105 expression seemed to be related to differentiation capacity, at least for adipogenesis. This study shows that ADSCs and fibroblasts are distinct cell types. These findings should be taken into account when using these two cell types in basic and therapeutic studies.engMary Ann Liebert, IncPolysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblastsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFibroblastsStromal CellsCélulas del EstromaFibroblastosCélulas Estromaisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9158/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALZych_Jaiesa_ICC_2014.pdfZych_Jaiesa_ICC_2014.pdfapplication/pdf654896https://www.arca.fiocruz.br/bitstream/icict/9158/2/Zych_Jaiesa_ICC_2014.pdfa298b6291876ed302886aafbac37b102MD52TEXTZych_Jaiesa_ICC_2014.pdf.txtZych_Jaiesa_ICC_2014.pdf.txtExtracted texttext/plain58119https://www.arca.fiocruz.br/bitstream/icict/9158/3/Zych_Jaiesa_ICC_2014.pdf.txtb6a7bed8a85bbbb38038188cc438f1e3MD53icict/91582021-12-23 16:34:04.297oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-12-23T19:34:04Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts
title	Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts
spellingShingle	Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts Zych, Jaiesa Fibroblasts Stromal Cells Células del Estroma Fibroblastos Células Estromais
title_short	Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts
title_full	Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts
title_fullStr	Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts
title_full_unstemmed	Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts
title_sort	Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts
author	Zych, Jaiesa
author_facet	Zych, Jaiesa Spangenberg, Lucia Stimamiglio, Marco Augusto Abud, Ana Paula Ressetti Shigunov, Patrícia Marchini, Fabrício Klerynton Kuligovski, Crisciele Cofré, Axel Helmut Rulf Schittini, Andressa V. Aguiar, Alessandra Melo de Senegaglia, Alexandra Cristina Brofman, Paulo Roberto Slud Goldenberg, Samuel Dallagiovanna, Bruno Naya, Hugo Dominguez, Alejandro Correa
author_role	author
author2	Spangenberg, Lucia Stimamiglio, Marco Augusto Abud, Ana Paula Ressetti Shigunov, Patrícia Marchini, Fabrício Klerynton Kuligovski, Crisciele Cofré, Axel Helmut Rulf Schittini, Andressa V. Aguiar, Alessandra Melo de Senegaglia, Alexandra Cristina Brofman, Paulo Roberto Slud Goldenberg, Samuel Dallagiovanna, Bruno Naya, Hugo Dominguez, Alejandro Correa
author2_role	author author author author author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Zych, Jaiesa Spangenberg, Lucia Stimamiglio, Marco Augusto Abud, Ana Paula Ressetti Shigunov, Patrícia Marchini, Fabrício Klerynton Kuligovski, Crisciele Cofré, Axel Helmut Rulf Schittini, Andressa V. Aguiar, Alessandra Melo de Senegaglia, Alexandra Cristina Brofman, Paulo Roberto Slud Goldenberg, Samuel Dallagiovanna, Bruno Naya, Hugo Dominguez, Alejandro Correa
dc.subject.en.En.fl_str_mv	Fibroblasts Stromal Cells
topic	Fibroblasts Stromal Cells Células del Estroma Fibroblastos Células Estromais
dc.subject.es.Es.fl_str_mv	Células del Estroma
dc.subject.decs.pt_BR.fl_str_mv	Fibroblastos Células Estromais
description	O artigo encontra-se disponível em acesso aberto no site do Editor.
publishDate	2014
dc.date.accessioned.fl_str_mv	2014-12-12T00:08:23Z
dc.date.issued.fl_str_mv	2014
dc.date.available.fl_str_mv	2015-11-15T06:30:04Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	ZYCH, Jaiesa et al. Polysome Profiling Shows the Identity of Human Adipose-Derived Stromal/Stem Cells in Detail and Clearly Distinguishes Them from Dermal Fibroblasts. Stem Cells and Development, v. 23, n. 22, p. 2791-2802, 2014.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/9158
dc.identifier.issn.none.fl_str_mv	1547-3287
dc.identifier.doi.pt_BR.fl_str_mv	10.1089/scd.2013.0496
identifier_str_mv	ZYCH, Jaiesa et al. Polysome Profiling Shows the Identity of Human Adipose-Derived Stromal/Stem Cells in Detail and Clearly Distinguishes Them from Dermal Fibroblasts. Stem Cells and Development, v. 23, n. 22, p. 2791-2802, 2014. 1547-3287 10.1089/scd.2013.0496
url	https://www.arca.fiocruz.br/handle/icict/9158
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Mary Ann Liebert, Inc
publisher.none.fl_str_mv	Mary Ann Liebert, Inc
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/9158/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/9158/2/Zych_Jaiesa_ICC_2014.pdf https://www.arca.fiocruz.br/bitstream/icict/9158/3/Zych_Jaiesa_ICC_2014.pdf.txt
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bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5 MD5
repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
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Polysome profiling shows the identity of human adipose-derived stromal/stem cells in detail and clearly distinguishes them from dermal fibroblasts

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