Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis

Detalhes bibliográficos
Autor(a) principal: Nacife, Valéria Pereira
Data de Publicação: 1998
Outros Autores: Meirelles, Maria de Nazareth Leal de, Silva Filho, Fernando Costa e
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43954
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.
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spelling Nacife, Valéria PereiraMeirelles, Maria de Nazareth Leal deSilva Filho, Fernando Costa e2020-10-13T13:00:56Z2020-10-13T13:00:56Z1998NACIFE, Valéria Pereira; MEIRELLES, Maria de Nazareth Leal de; SILVA FILHO, Fernando Costa e. Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n. 6, p. 807-814, Nov./Dec. 1998.0074-0276https://www.arca.fiocruz.br/handle/icict/4395410.1590/S0074-027619980006000201678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Didelphis marsupialisMonócitosCarga de superfícieTensão superficialMonocytesSurface chargeSurface tensionDidelphis marsupialisBasic Surface Properties of Mononuclear Cells from Didelphis marsupialisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.The electrostatic surface charge and surface tension of mononuclear cells/monocytes obtained from young and adult marsupials (Didelphis marsupialis) were investigated by using cationized ferritin and colloidal iron hydroxyde, whole cell electrophoresis, and measurements of contact angles. Anionic sites were found distributed throughout the entire investigated cell surfaces. The results revealed that the anionic character of the cells is given by electrostatic charges corresponding to -18.8 mV (cells from young animals) and -29.3 mV (cells from adult animals). The surface electrostatic charge decreased from 10 to 65.2% after treatment of the cells with each one of trypsin, neuraminidase and phospholipase C. The hydrophobic nature of the mononuclear cell surfaces studied by using the contact angle method revealed that both young and adult cells possess cell surfaces of high hidrofilicity since the angles formed with drops of saline water were 42.5°and 40.8°, respectively. Treatment of the cells with trypsin or neuraminidase rendered their surfaces more hydrophobic, suggesting that sialic acid-containing glycoproteins are responsible for most of the hydrophilicity observed in the mononuclear cell surfaces from D. marsupialis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43954/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALValeriaPereira_Nacif_etal_IOC_1998.pdfValeriaPereira_Nacif_etal_IOC_1998.pdfapplication/pdf609892https://www.arca.fiocruz.br/bitstream/icict/43954/2/ValeriaPereira_Nacif_etal_IOC_1998.pdf34f6d8f6e3b32deb78708f968424eebeMD52TEXTValeriaPereira_Nacif_etal_IOC_1998.pdf.txtValeriaPereira_Nacif_etal_IOC_1998.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis
title Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis
spellingShingle Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis
Nacife, Valéria Pereira
Didelphis marsupialis
Monócitos
Carga de superfície
Tensão superficial
Monocytes
Surface charge
Surface tension
Didelphis marsupialis
title_short Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis
title_full Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis
title_fullStr Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis
title_full_unstemmed Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis
title_sort Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis
author Nacife, Valéria Pereira
author_facet Nacife, Valéria Pereira
Meirelles, Maria de Nazareth Leal de
Silva Filho, Fernando Costa e
author_role author
author2 Meirelles, Maria de Nazareth Leal de
Silva Filho, Fernando Costa e
author2_role author
author
dc.contributor.author.fl_str_mv Nacife, Valéria Pereira
Meirelles, Maria de Nazareth Leal de
Silva Filho, Fernando Costa e
dc.subject.other.pt_BR.fl_str_mv Didelphis marsupialis
Monócitos
Carga de superfície
Tensão superficial
topic Didelphis marsupialis
Monócitos
Carga de superfície
Tensão superficial
Monocytes
Surface charge
Surface tension
Didelphis marsupialis
dc.subject.en.pt_BR.fl_str_mv Monocytes
Surface charge
Surface tension
Didelphis marsupialis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.
publishDate 1998
dc.date.issued.fl_str_mv 1998
dc.date.accessioned.fl_str_mv 2020-10-13T13:00:56Z
dc.date.available.fl_str_mv 2020-10-13T13:00:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NACIFE, Valéria Pereira; MEIRELLES, Maria de Nazareth Leal de; SILVA FILHO, Fernando Costa e. Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n. 6, p. 807-814, Nov./Dec. 1998.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43954
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761998000600020
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv NACIFE, Valéria Pereira; MEIRELLES, Maria de Nazareth Leal de; SILVA FILHO, Fernando Costa e. Basic Surface Properties of Mononuclear Cells from Didelphis marsupialis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n. 6, p. 807-814, Nov./Dec. 1998.
0074-0276
10.1590/S0074-02761998000600020
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43954
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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