EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis

Detalhes bibliográficos
Autor(a) principal: Melo, Christiane Bandeira
Data de Publicação: 2017
Outros Autores: Paiva, Ligia Almeida, Amorim, Natália R. T., Weller, Peter F., Bozza, Patrícia T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40886
Resumo: Acesso aberto em 20/04/2020 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774667/
id CRUZ_cba19ffbad5729dc23afad17650cbe97
oai_identifier_str oai:www.arca.fiocruz.br:icict/40886
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Melo, Christiane BandeiraPaiva, Ligia AlmeidaAmorim, Natália R. T.Weller, Peter F.Bozza, Patrícia T.2020-04-20T15:05:00Z2020-04-20T15:05:00Z2017MELO, Christianne Bandeira et al. EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis. Methods in Molecular Biology, v. 1554, p. 127-141, 2017.1064-3745https://www.arca.fiocruz.br/handle/icict/4088610.1007/978-1-4939-6759-9_61940-6029Acesso aberto em 20/04/2020 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774667/Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Harvard Medical Scholo. Department of Medicine. Beth Israel Deaconess Medical Center. Boston, MA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Eicosanoids are bioactive lipids derived from enzymatic metabolism of arachidonic acid via the cyclooxygenase (COX) and lipoxygenase (LOX) pathways. These lipids are newly formed and nonstorable molecules that have important roles in physiological and pathological processes. The particular interest to determine intracellular compartmentalization of eicosanoid-synthetic machinery has emerged as a key component in the regulation of eicosanoid synthesis and in delineating functional intracellular and extracellular actions of eicosanoids. In this chapter, we discuss the EicosaCell protocol, an assay that enables the intracellular detection and localization of eicosanoid lipid mediator-synthesizing compartments by means of a strategy to covalently cross-link and immobilize eicosanoids at their sites of synthesis followed by immunofluorescent-based localization of the targeted eicosanoid. EicosaCell assays have been successfully used to identify different intracellular compartments of synthesis of prostaglandins and leukotrienes upon cellular activation. This chapter covers basics of EicosaCell assay including its selection of reagents, immunodetection design as well as some troubleshooting recommendations.engHumana PressBiossínteseCarbodiimidaCompartimentalizaçãoEicosanóidesLeucotrienoGotículas lipídicasPerinuclearFagossomosProstaglandinaBiosynthesisCarbodiimideCompartmentalizationEDACEicosanoids;LeukotrieneLipid dropletsPerinuclearPhagosomesProstaglandinEicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40886/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPTBozza_CristianeBMelo_etal_IOC_2017.pdfPTBozza_CristianeBMelo_etal_IOC_2017.pdfapplication/pdf681268https://www.arca.fiocruz.br/bitstream/icict/40886/2/PTBozza_CristianeBMelo_etal_IOC_2017.pdf0894e8141819b9b05e1096b2d4dbade8MD52TEXTPTBozza_CristianeBMelo_etal_IOC_2017.pdf.txtPTBozza_CristianeBMelo_etal_IOC_2017.pdf.txtExtracted texttext/plain40873https://www.arca.fiocruz.br/bitstream/icict/40886/3/PTBozza_CristianeBMelo_etal_IOC_2017.pdf.txt0e7e833715761abaec057f4b62e98ea8MD53icict/408862022-06-24 13:08:44.211oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:08:44Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis
title EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis
spellingShingle EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis
Melo, Christiane Bandeira
Biossíntese
Carbodiimida
Compartimentalização
Eicosanóides
Leucotrieno
Gotículas lipídicas
Perinuclear
Fagossomos
Prostaglandina
Biosynthesis
Carbodiimide
Compartmentalization
EDAC
Eicosanoids;
Leukotriene
Lipid droplets
Perinuclear
Phagosomes
Prostaglandin
title_short EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis
title_full EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis
title_fullStr EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis
title_full_unstemmed EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis
title_sort EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis
author Melo, Christiane Bandeira
author_facet Melo, Christiane Bandeira
Paiva, Ligia Almeida
Amorim, Natália R. T.
Weller, Peter F.
Bozza, Patrícia T.
author_role author
author2 Paiva, Ligia Almeida
Amorim, Natália R. T.
Weller, Peter F.
Bozza, Patrícia T.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Melo, Christiane Bandeira
Paiva, Ligia Almeida
Amorim, Natália R. T.
Weller, Peter F.
Bozza, Patrícia T.
dc.subject.other.pt_BR.fl_str_mv Biossíntese
Carbodiimida
Compartimentalização
Eicosanóides
Leucotrieno
Gotículas lipídicas
Perinuclear
Fagossomos
Prostaglandina
topic Biossíntese
Carbodiimida
Compartimentalização
Eicosanóides
Leucotrieno
Gotículas lipídicas
Perinuclear
Fagossomos
Prostaglandina
Biosynthesis
Carbodiimide
Compartmentalization
EDAC
Eicosanoids;
Leukotriene
Lipid droplets
Perinuclear
Phagosomes
Prostaglandin
dc.subject.en.pt_BR.fl_str_mv Biosynthesis
Carbodiimide
Compartmentalization
EDAC
Eicosanoids;
Leukotriene
Lipid droplets
Perinuclear
Phagosomes
Prostaglandin
description Acesso aberto em 20/04/2020 - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774667/
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2020-04-20T15:05:00Z
dc.date.available.fl_str_mv 2020-04-20T15:05:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MELO, Christianne Bandeira et al. EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis. Methods in Molecular Biology, v. 1554, p. 127-141, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40886
dc.identifier.issn.pt_BR.fl_str_mv 1064-3745
dc.identifier.doi.none.fl_str_mv 10.1007/978-1-4939-6759-9_6
dc.identifier.eissn.none.fl_str_mv 1940-6029
identifier_str_mv MELO, Christianne Bandeira et al. EicosaCell: An Imaging-Based Assay to Identify Spatiotemporal Eicosanoid Synthesis. Methods in Molecular Biology, v. 1554, p. 127-141, 2017.
1064-3745
10.1007/978-1-4939-6759-9_6
1940-6029
url https://www.arca.fiocruz.br/handle/icict/40886
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/40886/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/40886/2/PTBozza_CristianeBMelo_etal_IOC_2017.pdf
https://www.arca.fiocruz.br/bitstream/icict/40886/3/PTBozza_CristianeBMelo_etal_IOC_2017.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
0894e8141819b9b05e1096b2d4dbade8
0e7e833715761abaec057f4b62e98ea8
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324864689373184

Registros relacionados