Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15606 |
Resumo: | This work was funded by the Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Programa de Desenvolvimento Tecnológico em Insumos para Saúde (PDTIS-Fiocruz). |
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González-Caballero, NataliaRodríguez-Vega, AndrésDias-Lopes, G.Valenzuela, J. G.Ribeiro, J. M.Carvalho, Paulo CostaValente, Richard HemmiBrazil, R. P.Cuervo, P.2016-09-05T14:38:28Z2016-09-05T14:38:28Z2014GONZÁLEZ-CABALLERO, N. et al. Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach. Journal of Proteomics, n. 96, p. 117-132, Jan. 2014.1874-3919https://www.arca.fiocruz.br/handle/icict/1560610.1016/j.jprot.2013.10.028This work was funded by the Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Programa de Desenvolvimento Tecnológico em Insumos para Saúde (PDTIS-Fiocruz).Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Pós-graduação Biologia Parasitaria. Rio de Janeiro, RJ, Brasil.Vector Molecular Biology Section. Laboratory of Malaria and Vector Research, National Institutes of Health Rockville. MD, USA.Vector Biology Section, Laboratory of Malaria and Vector Research. National Institutes of Health Rockville. MD, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil.In Latin America, Lutzomyia longipalpis is the main vector of the protozoan parasite Leishmania infantum, which is the causal agent of American Visceral Leishmaniasis. This insect uses male-produced pheromones for mate recognition. Elucidation of pheromone biogenesis or its regulation may enable molecular strategies for mating disruption and, consequently, the vector's population management. Motivated by our recent results of the transcriptomic characterization of the L. longipalpis pheromone gland, we performed a proteomic analysis of this tissue combining SDS-PAGE, and mass spectrometry followed by an integrative data analysis. Considering that annotated genome sequences of this sand fly are not available, we designed an alternative workflow searching MS/MS data against two customized databases using three search engines: Mascot, OMSSA and ProLuCID. A total of 542 proteins were confidently characterized, 445 of them using a Uniref100-insect protein database, and 97 using a transcript translated database. In addition, use of PEAKS for de novo peptide sequencing of MS/MS data confirmed ~90% identifications made with the combination of the three search engines. Our results include the identification of six of the seven enzymes of the mevalonate-pathway, plus the enzymes involved in sesquiterpenoid biosynthesis, all of which are proposed to be involved in pheromone production in L. longipalpis.porExpression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approachinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLutzomyia longipalpisMale pheromone glandMevalonate pathwayProteomeProteomicsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/15606/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALExpression of the mevalonate pathway enzymes in the Lutzomyia longipalpis.pdfExpression of the mevalonate pathway enzymes in the Lutzomyia longipalpis.pdfapplication/pdf858618https://www.arca.fiocruz.br/bitstream/icict/15606/2/Expression%20of%20the%20mevalonate%20pathway%20enzymes%20in%20the%20Lutzomyia%20longipalpis.pdfcdc1b00b0bb2e8a9bbd6091ab4388a73MD52TEXTExpression of the mevalonate pathway enzymes in the Lutzomyia longipalpis.pdf.txtExpression of the mevalonate pathway enzymes in the Lutzomyia longipalpis.pdf.txtExtracted texttext/plain83103https://www.arca.fiocruz.br/bitstream/icict/15606/3/Expression%20of%20the%20mevalonate%20pathway%20enzymes%20in%20the%20Lutzomyia%20longipalpis.pdf.txtd33e647bed3772b04bfe296623842fbeMD53icict/156062019-12-15 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| dc.title.pt_BR.fl_str_mv |
Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach |
| title |
Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach |
| spellingShingle |
Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach González-Caballero, Natalia Lutzomyia longipalpis Male pheromone gland Mevalonate pathway Proteome Proteomics |
| title_short |
Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach |
| title_full |
Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach |
| title_fullStr |
Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach |
| title_full_unstemmed |
Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach |
| title_sort |
Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach |
| author |
González-Caballero, Natalia |
| author_facet |
González-Caballero, Natalia Rodríguez-Vega, Andrés Dias-Lopes, G. Valenzuela, J. G. Ribeiro, J. M. Carvalho, Paulo Costa Valente, Richard Hemmi Brazil, R. P. Cuervo, P. |
| author_role |
author |
| author2 |
Rodríguez-Vega, Andrés Dias-Lopes, G. Valenzuela, J. G. Ribeiro, J. M. Carvalho, Paulo Costa Valente, Richard Hemmi Brazil, R. P. Cuervo, P. |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
González-Caballero, Natalia Rodríguez-Vega, Andrés Dias-Lopes, G. Valenzuela, J. G. Ribeiro, J. M. Carvalho, Paulo Costa Valente, Richard Hemmi Brazil, R. P. Cuervo, P. |
| dc.subject.en.pt_BR.fl_str_mv |
Lutzomyia longipalpis Male pheromone gland Mevalonate pathway Proteome Proteomics |
| topic |
Lutzomyia longipalpis Male pheromone gland Mevalonate pathway Proteome Proteomics |
| description |
This work was funded by the Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Programa de Desenvolvimento Tecnológico em Insumos para Saúde (PDTIS-Fiocruz). |
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2014 |
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2014 |
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2016-09-05T14:38:28Z |
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2016-09-05T14:38:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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| dc.identifier.citation.fl_str_mv |
GONZÁLEZ-CABALLERO, N. et al. Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach. Journal of Proteomics, n. 96, p. 117-132, Jan. 2014. |
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https://www.arca.fiocruz.br/handle/icict/15606 |
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1874-3919 |
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10.1016/j.jprot.2013.10.028 |
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GONZÁLEZ-CABALLERO, N. et al. Expression of the mevalonate pathway enzymes in the Lutzomyia longipalpis (Diptera: Psychodidae) sex pheromone gland demonstrated by an integrated proteomic approach. Journal of Proteomics, n. 96, p. 117-132, Jan. 2014. 1874-3919 10.1016/j.jprot.2013.10.028 |
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