Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode

Detalhes bibliográficos
Autor(a) principal: Rebello, Karina Mastropasqua
Data de Publicação: 2011
Outros Autores: Barros, Juliana S. L., Mota, Ester Maria, Carvalho, Paulo C., Perales, Jonas, Lenzi, Henrique Leonel, Ferreira, Ana G. C. Neves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3708
Resumo: This research was supported by Brazilian grants from Fiocruz (PAPES V and PDTIS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). We thank the staff of the Toxinology and Pathology Laboratories-IOC/Fiocruz for excellent technical assistance. We gratefully acknowledge Dr. André Teixeira S. Ferreira and Dr. Richard H. Valente (Toxinology Laboratory-IOC/Fiocruz) for their assistance with the mass spectrometric analysis. We also thank Monique R. O. Trugilho (Toxinology Laboratory-IOC/Fiocruz) for helping with 2-DE gel image analysis. We are grateful to Heloisa M. N. Diniz and Cristina S. Ferreira (Image Production and Treatment Service - IOC/Fiocruz) for processing the figures and creating the life cycle cartoon. KMR thanks CAPES for her PhD fellowship. PCC thanks CAPES/Fiocruz 30–2006 for his fellowship.
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spelling Rebello, Karina MastropasquaBarros, Juliana S. L.Mota, Ester MariaCarvalho, Paulo C.Perales, JonasLenzi, Henrique LeonelFerreira, Ana G. C. Neves2011-12-13T15:57:33Z2011-12-13T15:57:33Z2011REBELLO, Karina Mastropasqua et al. Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode. Journal of Proteomics, v. 74, n. 9, p. 1545-1559, 2011.1874-3919https://www.arca.fiocruz.br/handle/icict/370810.1016/j.jprot.2011.04.031This research was supported by Brazilian grants from Fiocruz (PAPES V and PDTIS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). We thank the staff of the Toxinology and Pathology Laboratories-IOC/Fiocruz for excellent technical assistance. We gratefully acknowledge Dr. André Teixeira S. Ferreira and Dr. Richard H. Valente (Toxinology Laboratory-IOC/Fiocruz) for their assistance with the mass spectrometric analysis. We also thank Monique R. O. Trugilho (Toxinology Laboratory-IOC/Fiocruz) for helping with 2-DE gel image analysis. We are grateful to Heloisa M. N. Diniz and Cristina S. Ferreira (Image Production and Treatment Service - IOC/Fiocruz) for processing the figures and creating the life cycle cartoon. KMR thanks CAPES for her PhD fellowship. PCC thanks CAPES/Fiocruz 30–2006 for his fellowship.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil / Proteomic Network. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Proteomic Network. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Proteomic Network. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ, Brasil / Proteomic Network. Rio de Janeiro, RJ, Brasil.Angiostrongylus costaricensis is a nematode helminth that causes an intestinal acute inflammatory process known as abdominal angiostrongyliasis, which is a poorly understood human disease occurring in Latin America. Our aim was to study the proteomic profiles of adult parasites focusing on immunogenic proteins. Total cellular extracts from both genders showed similar 2-DE profiles, with 60% of all protein spots focused between pH 5–7 and presenting molecular masses from 20.1 to 66 kDa. A total of 53 different dominant proteins were identified in our dataset and were mainly associated with the following over-represented Gene Ontology Biological Process terms: “macromolecule metabolic process”, “developmental process”, “response to stress”, and “biological regulation”. Female and male immunoblots showed similar patterns of reactive proteins. Immunoreactive spots identified by MALDI-PSD were found to represent heat shock proteins, a putative abnormal DAuer Formation family member, and galectins. To date, very few biochemical analyses have focused on the nematode Angiostrongylus costaricensis. As such, our results contribute to a better understanding of its biology and the mechanisms underlying the host–parasite relationship associated with this species. Moreover, our findings represent a first step in the search for candidate proteins for diagnostic assays and the treatment of this parasitic infection.engElsevierNematóidesAngiostrongylusProteínas ImunogênicasProteomaAngiostrongylus costaricensisNematodeProteomeImmunogenic ProteinsAngiostrongylusNematóidesProteomaComprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematodeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALComprehensive proteomic profiling of adult Angiostrongylus.pdfComprehensive proteomic profiling of adult Angiostrongylus.pdfapplication/pdf1766325https://www.arca.fiocruz.br/bitstream/icict/3708/1/Comprehensive%20proteomic%20profiling%20of%20adult%20Angiostrongylus.pdfb9dd765b435673e904949dbfcb4721faMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3708/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTComprehensive proteomic profiling of adult Angiostrongylus.pdf.txtComprehensive proteomic profiling of adult Angiostrongylus.pdf.txtExtracted texttext/plain75895https://www.arca.fiocruz.br/bitstream/icict/3708/5/Comprehensive%20proteomic%20profiling%20of%20adult%20Angiostrongylus.pdf.txt46959e6562bed245681e2e5085676484MD55THUMBNAILComprehensive proteomic profiling of adult Angiostrongylus.pdf.jpgComprehensive proteomic profiling of adult Angiostrongylus.pdf.jpgGenerated Thumbnailimage/jpeg2243https://www.arca.fiocruz.br/bitstream/icict/3708/4/Comprehensive%20proteomic%20profiling%20of%20adult%20Angiostrongylus.pdf.jpg2e029fc964135f19b7f4c31a746435c6MD54icict/37082023-07-28 09:55:17.829oai:www.arca.fiocruz.br:icict/3708Tk9OLUVYQ0xVU0lWRSBESVNUUklCVVRJT04gTElDRU5TRQoKQnkgc2lnbmluZyBhbmQgc3VibWl0dGluZyB0aGlzIGxpY2Vuc2UsIHlvdSAodGhlIGF1dGhvcihzKSBvciBjb3B5cmlnaHQKb3duZXIpIGdyYW50cyB0byBEU3BhY2UgVW5pdmVyc2l0eSAoRFNVKSB0aGUgbm9uLWV4Y2x1c2l2ZSByaWdodCB0byByZXByb2R1Y2UsCnRyYW5zbGF0ZSAoYXMgZGVmaW5lZCBiZWxvdyksIGFuZC9vciBkaXN0cmlidXRlIHlvdXIgc3VibWlzc2lvbiAoaW5jbHVkaW5nCnRoZSBhYnN0cmFjdCkgd29ybGR3aWRlIGluIHByaW50IGFuZCBlbGVjdHJvbmljIGZvcm1hdCBhbmQgaW4gYW55IG1lZGl1bSwKaW5jbHVkaW5nIGJ1dCBub3QgbGltaXRlZCB0byBhdWRpbyBvciB2aWRlby4KCllvdSBhZ3JlZSB0aGF0IERTVSBtYXksIHdpdGhvdXQgY2hhbmdpbmcgdGhlIGNvbnRlbnQsIHRyYW5zbGF0ZSB0aGUKc3VibWlzc2lvbiB0byBhbnkgbWVkaXVtIG9yIGZvcm1hdCBmb3IgdGhlIHB1cnBvc2Ugb2YgcHJlc2VydmF0aW9uLgoKWW91IGFsc28gYWdyZWUgdGhhdCBEU1UgbWF5IGtlZXAgbW9yZSB0aGFuIG9uZSBjb3B5IG9mIHRoaXMgc3VibWlzc2lvbiBmb3IKcHVycG9zZXMgb2Ygc2VjdXJpdHksIGJhY2stdXAgYW5kIHByZXNlcnZhdGlvbi4KCllvdSByZXByZXNlbnQgdGhhdCB0aGUgc3VibWlzc2lvbiBpcyB5b3VyIG9yaWdpbmFsIHdvcmssIGFuZCB0aGF0IHlvdSBoYXZlCnRoZSByaWdodCB0byBncmFudCB0aGUgcmlnaHRzIGNvbnRhaW5lZCBpbiB0aGlzIGxpY2Vuc2UuIFlvdSBhbHNvIHJlcHJlc2VudAp0aGF0IHlvdXIgc3VibWlzc2lvbiBkb2VzIG5vdCwgdG8gdGhlIGJlc3Qgb2YgeW91ciBrbm93bGVkZ2UsIGluZnJpbmdlIHVwb24KYW55b25lJ3MgY29weXJpZ2h0LgoKSWYgdGhlIHN1Ym1pc3Npb24gY29udGFpbnMgbWF0ZXJpYWwgZm9yIHdoaWNoIHlvdSBkbyBub3QgaG9sZCBjb3B5cmlnaHQsCnlvdSByZXByZXNlbnQgdGhhdCB5b3UgaGF2ZSBvYnRhaW5lZCB0aGUgdW5yZXN0cmljdGVkIHBlcm1pc3Npb24gb2YgdGhlCmNvcHlyaWdodCBvd25lciB0byBncmFudCBEU1UgdGhlIHJpZ2h0cyByZXF1aXJlZCBieSB0aGlzIGxpY2Vuc2UsIGFuZCB0aGF0CnN1Y2ggdGhpcmQtcGFydHkgb3duZWQgbWF0ZXJpYWwgaXMgY2xlYXJseSBpZGVudGlmaWVkIGFuZCBhY2tub3dsZWRnZWQKd2l0aGluIHRoZSB0ZXh0IG9yIGNvbnRlbnQgb2YgdGhlIHN1Ym1pc3Npb24uCgpJRiBUSEUgU1VCTUlTU0lPTiBJUyBCQVNFRCBVUE9OIFdPUksgVEhBVCBIQVMgQkVFTiBTUE9OU09SRUQgT1IgU1VQUE9SVEVECkJZIEFOIEFHRU5DWSBPUiBPUkdBTklaQVRJT04gT1RIRVIgVEhBTiBEU1UsIFlPVSBSRVBSRVNFTlQgVEhBVCBZT1UgSEFWRQpGVUxGSUxMRUQgQU5ZIFJJR0hUIE9GIFJFVklFVyBPUiBPVEhFUiBPQkxJR0FUSU9OUyBSRVFVSVJFRCBCWSBTVUNICkNPTlRSQUNUIE9SIEFHUkVFTUVOVC4KCkRTVSB3aWxsIGNsZWFybHkgaWRlbnRpZnkgeW91ciBuYW1lKHMpIGFzIHRoZSBhdXRob3Iocykgb3Igb3duZXIocykgb2YgdGhlCnN1Ym1pc3Npb24sIGFuZCB3aWxsIG5vdCBtYWtlIGFueSBhbHRlcmF0aW9uLCBvdGhlciB0aGFuIGFzIGFsbG93ZWQgYnkgdGhpcwpsaWNlbnNlLCB0byB5b3VyIHN1Ym1pc3Npb24uCg==Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-28T12:55:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode
title Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode
spellingShingle Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode
Rebello, Karina Mastropasqua
Nematóides
Angiostrongylus
Proteínas Imunogênicas
Proteoma
Angiostrongylus costaricensis
Nematode
Proteome
Immunogenic Proteins
Angiostrongylus
Nematóides
Proteoma
title_short Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode
title_full Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode
title_fullStr Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode
title_full_unstemmed Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode
title_sort Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode
author Rebello, Karina Mastropasqua
author_facet Rebello, Karina Mastropasqua
Barros, Juliana S. L.
Mota, Ester Maria
Carvalho, Paulo C.
Perales, Jonas
Lenzi, Henrique Leonel
Ferreira, Ana G. C. Neves
author_role author
author2 Barros, Juliana S. L.
Mota, Ester Maria
Carvalho, Paulo C.
Perales, Jonas
Lenzi, Henrique Leonel
Ferreira, Ana G. C. Neves
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rebello, Karina Mastropasqua
Barros, Juliana S. L.
Mota, Ester Maria
Carvalho, Paulo C.
Perales, Jonas
Lenzi, Henrique Leonel
Ferreira, Ana G. C. Neves
dc.subject.other.pt_BR.fl_str_mv Nematóides
Angiostrongylus
Proteínas Imunogênicas
Proteoma
topic Nematóides
Angiostrongylus
Proteínas Imunogênicas
Proteoma
Angiostrongylus costaricensis
Nematode
Proteome
Immunogenic Proteins
Angiostrongylus
Nematóides
Proteoma
dc.subject.en.pt_BR.fl_str_mv Angiostrongylus costaricensis
Nematode
Proteome
Immunogenic Proteins
dc.subject.decs.pt_BR.fl_str_mv Angiostrongylus
Nematóides
Proteoma
description This research was supported by Brazilian grants from Fiocruz (PAPES V and PDTIS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). We thank the staff of the Toxinology and Pathology Laboratories-IOC/Fiocruz for excellent technical assistance. We gratefully acknowledge Dr. André Teixeira S. Ferreira and Dr. Richard H. Valente (Toxinology Laboratory-IOC/Fiocruz) for their assistance with the mass spectrometric analysis. We also thank Monique R. O. Trugilho (Toxinology Laboratory-IOC/Fiocruz) for helping with 2-DE gel image analysis. We are grateful to Heloisa M. N. Diniz and Cristina S. Ferreira (Image Production and Treatment Service - IOC/Fiocruz) for processing the figures and creating the life cycle cartoon. KMR thanks CAPES for her PhD fellowship. PCC thanks CAPES/Fiocruz 30–2006 for his fellowship.
publishDate 2011
dc.date.accessioned.fl_str_mv 2011-12-13T15:57:33Z
dc.date.available.fl_str_mv 2011-12-13T15:57:33Z
dc.date.issued.fl_str_mv 2011
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REBELLO, Karina Mastropasqua et al. Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode. Journal of Proteomics, v. 74, n. 9, p. 1545-1559, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3708
dc.identifier.issn.none.fl_str_mv 1874-3919
dc.identifier.doi.none.fl_str_mv 10.1016/j.jprot.2011.04.031
identifier_str_mv REBELLO, Karina Mastropasqua et al. Comprehensive proteomic profiling of adult Angiostrongylus costaricensis, a human parasitic nematode. Journal of Proteomics, v. 74, n. 9, p. 1545-1559, 2011.
1874-3919
10.1016/j.jprot.2011.04.031
url https://www.arca.fiocruz.br/handle/icict/3708
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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