Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria

Detalhes bibliográficos
Autor(a) principal: Franklin, Bernardo Simões
Data de Publicação: 2011
Outros Autores: Vitorino, Bárbara Larissa Ferreira, Coelho, Helena Cristina Cardoso, Menezes Neto, Armando, Santos, Marina L. S, Campos, Fernanda Magalhães Freire, Brito, Cristiana Ferreira Alves de, Fontes, Cor de Jesus Fernandes, Lacerda, Marcus Vinícius Guimarães de, Carvalho, Luzia Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8685
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, Brazil
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spelling Franklin, Bernardo SimõesVitorino, Bárbara Larissa FerreiraCoelho, Helena Cristina CardosoMenezes Neto, ArmandoSantos, Marina L. SCampos, Fernanda Magalhães FreireBrito, Cristiana Ferreira Alves deFontes, Cor de Jesus FernandesLacerda, Marcus Vinícius Guimarães deCarvalho, Luzia Helena2014-10-28T17:56:53Z2014-10-28T17:56:53Z2011FRANKLIN, Bernardo Simões et al. Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria. PLoS One 6(5): e19842, 2011.1932-6203https://www.arca.fiocruz.br/handle/icict/868510.1371/journal.pone.0019842engPublic Library of SciencePlasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malariainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, BrazilFundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, BrazilUniversidade Federal de Mato Grosso. Departamento de Clínica Médica. Cuiabá, MT, BrazilFundação de Medicina Tropical Dr. Heitor Vieira Dourado. Gerência de Malária. Manaus, AM, BrazilFundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, BrazilBackground: Given the increasing evidence of Plasmodium vivax infections associated with severe and fatal disease, the identification of sensitive and reliable markers for vivax severity is crucial to improve patient care. Circulating nucleic acids (CNAs) have been increasingly recognized as powerful diagnostic and prognostic tools for various inflammatory diseases and tumors as their plasma concentrations increase according to malignancy. Given the marked inflammatory status of P. vivax infection, we investigated here the usefulness of CNAs as biomarkers for malaria morbidity. Methods and Findings: CNAs levels in plasma from twenty-one acute P. vivax malaria patients from the Brazilian Amazon and 14 malaria non-exposed healthy donors were quantified by two different methodologies: amplification of the human telomerase reverse transcriptase (hTERT) genomic sequence by quantitative real time PCR (qPCR), and the fluorometric dsDNA quantification by Pico Green. CNAs levels were significantly increased in plasma from P. vivax patients as compared to healthy donors (p<0.0001). Importantly, plasma CNAs levels were strongly associated with vivax morbidity (p<0.0001), including a drop in platelet counts (p = 0.0021). These findings were further sustained when we assessed CNAS levels in plasma samples from 14 additional P. vivax patients of a different endemic area in Brazil, in which CNAS levels strongly correlated with thrombocytopenia (p = 0.0072). We further show that plasma CNAs levels decrease and reach physiological levels after antimalarial treatment. Although we found both host and parasite specific genomic sequences circulating in plasma, only host CNAs clearly reflected the clinical spectrum of P. vivax malaria. Conclusions: Here, we provide the first evidence of increased plasma CNAs levels in malaria patients and reveal their potential as sensitive biomarkers for vivax malaria morbidity.Blood plasmaMalarial parasitesParasitic diseasesPlasmodiumPlateletsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8685/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALPlasma Circulating Nucleic Acids Levels Increase.pdfPlasma Circulating Nucleic Acids Levels Increase.pdfapplication/pdf534825https://www.arca.fiocruz.br/bitstream/icict/8685/2/Plasma%20Circulating%20Nucleic%20Acids%20Levels%20Increase.pdf0599c0644907584e86f82cbb4929c4a4MD52TEXTPlasma Circulating Nucleic Acids Levels Increase.pdf.txtPlasma Circulating Nucleic Acids Levels Increase.pdf.txtExtracted texttext/plain48082https://www.arca.fiocruz.br/bitstream/icict/8685/3/Plasma%20Circulating%20Nucleic%20Acids%20Levels%20Increase.pdf.txt5840f031875a9efb8889524a2864626eMD53icict/86852019-06-19 10:12:15.899oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:12:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria
title Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria
spellingShingle Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria
Franklin, Bernardo Simões
Blood plasma
Malarial parasites
Parasitic diseases
Plasmodium
Platelets
title_short Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria
title_full Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria
title_fullStr Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria
title_full_unstemmed Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria
title_sort Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria
author Franklin, Bernardo Simões
author_facet Franklin, Bernardo Simões
Vitorino, Bárbara Larissa Ferreira
Coelho, Helena Cristina Cardoso
Menezes Neto, Armando
Santos, Marina L. S
Campos, Fernanda Magalhães Freire
Brito, Cristiana Ferreira Alves de
Fontes, Cor de Jesus Fernandes
Lacerda, Marcus Vinícius Guimarães de
Carvalho, Luzia Helena
author_role author
author2 Vitorino, Bárbara Larissa Ferreira
Coelho, Helena Cristina Cardoso
Menezes Neto, Armando
Santos, Marina L. S
Campos, Fernanda Magalhães Freire
Brito, Cristiana Ferreira Alves de
Fontes, Cor de Jesus Fernandes
Lacerda, Marcus Vinícius Guimarães de
Carvalho, Luzia Helena
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Franklin, Bernardo Simões
Vitorino, Bárbara Larissa Ferreira
Coelho, Helena Cristina Cardoso
Menezes Neto, Armando
Santos, Marina L. S
Campos, Fernanda Magalhães Freire
Brito, Cristiana Ferreira Alves de
Fontes, Cor de Jesus Fernandes
Lacerda, Marcus Vinícius Guimarães de
Carvalho, Luzia Helena
dc.subject.en.pt_BR.fl_str_mv Blood plasma
Malarial parasites
Parasitic diseases
Plasmodium
Platelets
topic Blood plasma
Malarial parasites
Parasitic diseases
Plasmodium
Platelets
description Fundação Oswaldo Cruz. Centro de Pesquisa René. Laboratório de Malária. Belo Horizonte, MG, Brazil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-10-28T17:56:53Z
dc.date.available.fl_str_mv 2014-10-28T17:56:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRANKLIN, Bernardo Simões et al. Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria. PLoS One 6(5): e19842, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8685
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0019842
identifier_str_mv FRANKLIN, Bernardo Simões et al. Plasma Circulating Nucleic Acids Levels Increase According to the Morbidity of Plasmodium vivax Malaria. PLoS One 6(5): e19842, 2011.
1932-6203
10.1371/journal.pone.0019842
url https://www.arca.fiocruz.br/handle/icict/8685
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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