Distribution and analysis of surface charge on brain endothelium in vitro and in situ

Detalhes bibliográficos
Autor(a) principal: dosSantos, Washington Luis Conrado
Data de Publicação: 1995
Outros Autores: Rahman, Jameel, Klein, Nigel, Male, David K
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20034
Resumo: dosSantos, Washington Luis Conrado “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling dosSantos, Washington Luis ConradoRahman, JameelKlein, NigelMale, David K2017-07-11T12:00:11Z2017-07-11T12:00:11Z1995dosSANTOS, W. L. C. et al. Distribution and analysis of surface charge on brain endothelium in vitro and in situ. Acta Neuropathologica, v. 90, p. 305-311, 1995.0001-6322https://www.arca.fiocruz.br/handle/icict/20034dosSantos, Washington Luis Conrado “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Brazilian Research Council (CNPq) and the Multiple Sclerosis Society of Great BritainInstitute of Psychiatry. Department of Neuropathology. London, UKInstitute of Psychiatry. Department of Neuropathology. London, UKInstitute of Child Health. Molecular Immunology Unit. London, UKInstitute of Psychiatry. Department of Neuropathology. London, UKVascular endothelial cells are associated with a number of anionic molecules. These anions are important in endothelial function, particularly in regulating permeability, haemostasis and cellular traffic. To explore the nature and distribution of anions on the brain endothelial cell (BEC) surface, we have examined rat brain endothelium in culture, and in situ. The anionic sites were probed with cationic colloidal gold and cationised ferritin, and visualised by light microscopy. Additionally we compared the distribution of the anionic sites on BEC with that present on other endothelial cell types in culture. The predominant anion detected on BEC was heparan sulphate (HS). This was distributed throughout the cell membrane, but was most densely associated with intercellular junctions. This pattern was distinct from the anionic locations observed in endothelia from aorta and epididymal fat microvessels. The distribution of anions was dependent on the age of cultured cells, with only minimal levels of HS seen at the periphery of younger cells. The nature and distribution of negative charge was different in situ. Here, sialic acid was the major surface anion, with only a small contribution from HS. The significance of these findings are discussed in relation to endothelial function in normal tissue and in pathological conditions.engSpringer VerlagEndotélioCarga negativaGlicosaminoglicanosSulfato de HeparanÁcido siálicoEndotheliumNegative chargeGlycosaminoglycansHeparan sulfateSialic acidDistribution and analysis of surface charge on brain endothelium in vitro and in situinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20034/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALdos Santos WLC Distribution and analysis....pdfdos Santos WLC Distribution and 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dc.title.pt_BR.fl_str_mv Distribution and analysis of surface charge on brain endothelium in vitro and in situ
title Distribution and analysis of surface charge on brain endothelium in vitro and in situ
spellingShingle Distribution and analysis of surface charge on brain endothelium in vitro and in situ
dosSantos, Washington Luis Conrado
Endotélio
Carga negativa
Glicosaminoglicanos
Sulfato de Heparan
Ácido siálico
Endothelium
Negative charge
Glycosaminoglycans
Heparan sulfate
Sialic acid
title_short Distribution and analysis of surface charge on brain endothelium in vitro and in situ
title_full Distribution and analysis of surface charge on brain endothelium in vitro and in situ
title_fullStr Distribution and analysis of surface charge on brain endothelium in vitro and in situ
title_full_unstemmed Distribution and analysis of surface charge on brain endothelium in vitro and in situ
title_sort Distribution and analysis of surface charge on brain endothelium in vitro and in situ
author dosSantos, Washington Luis Conrado
author_facet dosSantos, Washington Luis Conrado
Rahman, Jameel
Klein, Nigel
Male, David K
author_role author
author2 Rahman, Jameel
Klein, Nigel
Male, David K
author2_role author
author
author
dc.contributor.author.fl_str_mv dosSantos, Washington Luis Conrado
Rahman, Jameel
Klein, Nigel
Male, David K
dc.subject.other.pt_BR.fl_str_mv Endotélio
Carga negativa
Glicosaminoglicanos
Sulfato de Heparan
Ácido siálico
topic Endotélio
Carga negativa
Glicosaminoglicanos
Sulfato de Heparan
Ácido siálico
Endothelium
Negative charge
Glycosaminoglycans
Heparan sulfate
Sialic acid
dc.subject.en.pt_BR.fl_str_mv Endothelium
Negative charge
Glycosaminoglycans
Heparan sulfate
Sialic acid
description dosSantos, Washington Luis Conrado “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 1995
dc.date.issued.fl_str_mv 1995
dc.date.accessioned.fl_str_mv 2017-07-11T12:00:11Z
dc.date.available.fl_str_mv 2017-07-11T12:00:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv dosSANTOS, W. L. C. et al. Distribution and analysis of surface charge on brain endothelium in vitro and in situ. Acta Neuropathologica, v. 90, p. 305-311, 1995.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20034
dc.identifier.issn.pt_BR.fl_str_mv 0001-6322
identifier_str_mv dosSANTOS, W. L. C. et al. Distribution and analysis of surface charge on brain endothelium in vitro and in situ. Acta Neuropathologica, v. 90, p. 305-311, 1995.
0001-6322
url https://www.arca.fiocruz.br/handle/icict/20034
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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