The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection

Detalhes bibliográficos
Autor(a) principal: Silva Junior, Elias Barbosa da
Data de Publicação: 2021
Outros Autores: Cruz, Luan Firmino, Oliveira, Joyce Cristina Guimarães de, Medeiros, Juliana Valente Rodrigues de, Nascimento, Danielle de Oliveira, Lima, Matheus Freire de, Gitirana, Lycia de Brito, Morrot, Alexandre, Previato, Jose Osvaldo, Previato, Lucia Mendonça, Ricardo, Debora Decote, Guedes, Herbert Leonel de Matos, Lima, Celio Geraldo Freire de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/47895
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
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spelling Silva Junior, Elias Barbosa daCruz, Luan FirminoOliveira, Joyce Cristina Guimarães deMedeiros, Juliana Valente Rodrigues deNascimento, Danielle de OliveiraLima, Matheus Freire deGitirana, Lycia de BritoMorrot, AlexandrePreviato, Jose OsvaldoPreviato, Lucia MendonçaRicardo, Debora DecoteGuedes, Herbert Leonel de MatosLima, Celio Geraldo Freire de2021-06-28T13:36:23Z2021-06-28T13:36:23Z2021SILVA JUNIOR, Elias Barbosa da et al. The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection. Scientifc Reports, v. 11, n.1407, 11 p, 2021.2045-2322https://www.arca.fiocruz.br/handle/icict/4789510.1038/s41598-021-80959-5engNature ResarchReceptor Toll-Like 9CriptococoseCryptococcus gattiiInfecçãoToll‑like receptor 9Murine modelCryptococcus gattiiInfectionReceptor Toll-Like 9CriptococoseThe role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro.. Faculdade de Medicina. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Toll-like receptor 9 (TLR9) is crucial to the host immune response against fungi, such as Candida albicans, Aspergillus fumigatus and Cryptococcus neoformans, but its importance in Cryptococcus gattii infection is unknown. Our study aimed to understand the role of TLR9 during the course of experimental C. gattii infection in vivo, considering that the cryptococcal DNA interaction with the receptor could contribute to host immunity even in an extremely susceptible model. We inoculated C57BL/6 (WT) and TLR9 knock-out (TLR9−/−) mice intratracheally with 104 C. gattii yeast cells. TLR9−/− mice had a higher mortality rate compared to WT mice and more yeast cells that had abnormal size, known as titan cells, in the lungs. TLR9−/− mice also had a greater number of CFUs in the spleen and brain than WT mice, in addition to having lower levels of IFN-γ and IL-17 in the lung. With these markers of aggressive cryptococcosis, we can state that TLR9−/− mice are more susceptible to C. gattii, probably due to a mechanism associated with the decrease of a Th1 and Th17-type immune response that promotes the formation of titan cells in the lungs. Therefore, our results indicate the participation of TLR9 in murine resistance to C. gattii infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47895/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLuanFCruz_JulianaMedeiros_etal_IOC_2021.pdfLuanFCruz_JulianaMedeiros_etal_IOC_2021.pdfapplication/pdf1685105https://www.arca.fiocruz.br/bitstream/icict/47895/2/LuanFCruz_JulianaMedeiros_etal_IOC_2021.pdf6cbb465ce44389260c2f1101cfbaa190MD52TEXTLuanFCruz_JulianaMedeiros_etal_IOC_2021.pdf.txtLuanFCruz_JulianaMedeiros_etal_IOC_2021.pdf.txtExtracted texttext/plain52933https://www.arca.fiocruz.br/bitstream/icict/47895/3/LuanFCruz_JulianaMedeiros_etal_IOC_2021.pdf.txt93134ba9a08b537537b90ef586f47ce5MD53icict/478952022-06-24 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dc.title.pt_BR.fl_str_mv The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection
title The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection
spellingShingle The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection
Silva Junior, Elias Barbosa da
Receptor Toll-Like 9
Criptococose
Cryptococcus gattii
Infecção
Toll‑like receptor 9
Murine model
Cryptococcus gattii
Infection
Receptor Toll-Like 9
Criptococose
title_short The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection
title_full The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection
title_fullStr The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection
title_full_unstemmed The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection
title_sort The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection
author Silva Junior, Elias Barbosa da
author_facet Silva Junior, Elias Barbosa da
Cruz, Luan Firmino
Oliveira, Joyce Cristina Guimarães de
Medeiros, Juliana Valente Rodrigues de
Nascimento, Danielle de Oliveira
Lima, Matheus Freire de
Gitirana, Lycia de Brito
Morrot, Alexandre
Previato, Jose Osvaldo
Previato, Lucia Mendonça
Ricardo, Debora Decote
Guedes, Herbert Leonel de Matos
Lima, Celio Geraldo Freire de
author_role author
author2 Cruz, Luan Firmino
Oliveira, Joyce Cristina Guimarães de
Medeiros, Juliana Valente Rodrigues de
Nascimento, Danielle de Oliveira
Lima, Matheus Freire de
Gitirana, Lycia de Brito
Morrot, Alexandre
Previato, Jose Osvaldo
Previato, Lucia Mendonça
Ricardo, Debora Decote
Guedes, Herbert Leonel de Matos
Lima, Celio Geraldo Freire de
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva Junior, Elias Barbosa da
Cruz, Luan Firmino
Oliveira, Joyce Cristina Guimarães de
Medeiros, Juliana Valente Rodrigues de
Nascimento, Danielle de Oliveira
Lima, Matheus Freire de
Gitirana, Lycia de Brito
Morrot, Alexandre
Previato, Jose Osvaldo
Previato, Lucia Mendonça
Ricardo, Debora Decote
Guedes, Herbert Leonel de Matos
Lima, Celio Geraldo Freire de
dc.subject.other.pt_BR.fl_str_mv Receptor Toll-Like 9
Criptococose
Cryptococcus gattii
Infecção
topic Receptor Toll-Like 9
Criptococose
Cryptococcus gattii
Infecção
Toll‑like receptor 9
Murine model
Cryptococcus gattii
Infection
Receptor Toll-Like 9
Criptococose
dc.subject.en.pt_BR.fl_str_mv Toll‑like receptor 9
Murine model
Cryptococcus gattii
Infection
dc.subject.decs.pt_BR.fl_str_mv Receptor Toll-Like 9
Criptococose
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-06-28T13:36:23Z
dc.date.available.fl_str_mv 2021-06-28T13:36:23Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA JUNIOR, Elias Barbosa da et al. The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection. Scientifc Reports, v. 11, n.1407, 11 p, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/47895
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-021-80959-5
identifier_str_mv SILVA JUNIOR, Elias Barbosa da et al. The role ofToll‑like receptor 9 in a murine model of Cryptococcus gattii infection. Scientifc Reports, v. 11, n.1407, 11 p, 2021.
2045-2322
10.1038/s41598-021-80959-5
url https://www.arca.fiocruz.br/handle/icict/47895
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Resarch
publisher.none.fl_str_mv Nature Resarch
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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