Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14937 http://dx.doi.org/10.4236/wjcd.2013.38080 |
Resumo: | Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil. |
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Vieira, DeusileneGauthier, MarieNicolete, Larissa Deadame de FigueiredoSantos, Alcione Oliveira dosPicelli, CarinaHonda, EduardoParanhos-Baccalà, GlauciaVernet, GuySalcedo, Juan Miguel Villalobos2016-07-14T15:31:45Z2016-07-15T18:40:32Z2016-07-14T15:31:45Z2016-07-15T18:40:32Z2013VIEIRA, Deusilene et al. Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization. World Journal of Cardiovascular Diseases, v. 3, p. 506-511, 2013.https://www.arca.fiocruz.br/handle/icict/14937http://dx.doi.org/10.4236/wjcd.2013.38080engWorld Journal of Cardiovascular DiseasesChronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterizationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.Fondation Mérieux. Emerging Pathogens Laboratory. IFR 128 BioSciences. Gerland, Lyon, France.Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.Fondation Mérieux. Emerging Pathogens Laboratory. IFR 128 BioSciences. Gerland, Lyon, France.Fondation Mérieux. Emerging Pathogens Laboratory. IFR 128 BioSciences. Gerland, Lyon, France.Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil.The Amazon region is considered to be a high endemic area for Hepatitis B Virus (HBV) infections, Rondônia state having the highest prevalence. The aim of this study was to identify molecular genotypes and mutations in the S gene region of HBV viral genomes from 20 patients using a DNA microarray. Results: Serological tests showed that 88% of patients were HBeAg negative, 82% had anti-HBe antibodies and 33% were co-infected with Hepatitis Delta Virus. Sixteen percent of the patients were considered cirrhotic, and 11% have been transfused. The microarray technique identified the genotypes A (4 patients), D (7 patients) and F (7 patients) in 18 samples. Mutations were detected in all 3 genotypes and, overall, A159G, which has been associated with a reduced antigenicity of the virus, was detected most frequently. In genotype A, G119E was the most frequently detected mutation followed by mutations A159G, F134Y, W172C, Y161F and T143S. A159G was detected in all genotype D and F samples followed by mutations T143S, Y161F, N131T, T114S and G119E in genotype D and mutations T143S, Y161F, N131T, T114S and G119E in genotype F. Conclusion: The analysis of mutations repartition among genotypes suggests that some of them are preferentially or exclusively associated with genotype A, D or F. This type of tool is adapted for clinical and therapy monitoring of patient as well as for molecular epidemiology research on HBV.Hepatitis B VirusMutant DetectionDNA MicroarrayAmazon Regioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTChronic hepatitis B liver.pdf.txtChronic hepatitis B liver.pdf.txtExtracted texttext/plain25259https://www.arca.fiocruz.br/bitstream/icict/14937/4/Chronic%20hepatitis%20B%20liver.pdf.txtb784c3498783414824a7e653920c297dMD54ORIGINALChronic hepatitis B liver.pdfapplication/pdf233232https://www.arca.fiocruz.br/bitstream/icict/14937/2/Chronic%20hepatitis%20B%20liver.pdf9a815d9a6920f8d928f82785cc1aba7fMD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14937/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/149372019-04-18 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| dc.title.pt_BR.fl_str_mv |
Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization |
| title |
Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization |
| spellingShingle |
Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization Vieira, Deusilene Hepatitis B Virus Mutant Detection DNA Microarray Amazon Region |
| title_short |
Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization |
| title_full |
Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization |
| title_fullStr |
Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization |
| title_full_unstemmed |
Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization |
| title_sort |
Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization |
| author |
Vieira, Deusilene |
| author_facet |
Vieira, Deusilene Gauthier, Marie Nicolete, Larissa Deadame de Figueiredo Santos, Alcione Oliveira dos Picelli, Carina Honda, Eduardo Paranhos-Baccalà, Glaucia Vernet, Guy Salcedo, Juan Miguel Villalobos |
| author_role |
author |
| author2 |
Gauthier, Marie Nicolete, Larissa Deadame de Figueiredo Santos, Alcione Oliveira dos Picelli, Carina Honda, Eduardo Paranhos-Baccalà, Glaucia Vernet, Guy Salcedo, Juan Miguel Villalobos |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vieira, Deusilene Gauthier, Marie Nicolete, Larissa Deadame de Figueiredo Santos, Alcione Oliveira dos Picelli, Carina Honda, Eduardo Paranhos-Baccalà, Glaucia Vernet, Guy Salcedo, Juan Miguel Villalobos |
| dc.subject.en.eng.fl_str_mv |
Hepatitis B Virus Mutant Detection DNA Microarray Amazon Region |
| topic |
Hepatitis B Virus Mutant Detection DNA Microarray Amazon Region |
| description |
Fundação Oswaldo Cruz. Research Center for Tropical Medicine. Tropical Pathology Research Institute-CEPEM. Porto Velho, RO, Brasil. |
| publishDate |
2013 |
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2013 |
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2016-07-14T15:31:45Z 2016-07-15T18:40:32Z |
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2016-07-14T15:31:45Z 2016-07-15T18:40:32Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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VIEIRA, Deusilene et al. Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization. World Journal of Cardiovascular Diseases, v. 3, p. 506-511, 2013. |
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https://www.arca.fiocruz.br/handle/icict/14937 |
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http://dx.doi.org/10.4236/wjcd.2013.38080 |
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VIEIRA, Deusilene et al. Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization. World Journal of Cardiovascular Diseases, v. 3, p. 506-511, 2013. |
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https://www.arca.fiocruz.br/handle/icict/14937 http://dx.doi.org/10.4236/wjcd.2013.38080 |
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World Journal of Cardiovascular Diseases |
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World Journal of Cardiovascular Diseases |
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