McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication

Detalhes bibliográficos
Autor(a) principal: Nogueira, Yeda L.
Data de Publicação: 2003
Outros Autores: Oliveira, Carmen A. Freitas, Ferreira, Antonio Gomes P., Nakamura, Paulo M., Magnanelli, Antonio Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33169
Resumo: Instituto Adolfo Lutz. São Paulo, SP, Brasil.
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spelling Nogueira, Yeda L.Oliveira, Carmen A. FreitasFerreira, Antonio Gomes P.Nakamura, Paulo M.Magnanelli, Antonio Carlos2019-05-22T12:59:50Z2019-05-22T12:59:50Z2003NOGUEIRA, Yeda L. et al . McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication. Rev. Inst. Med. Trop. S. Paulo, São Paulo, v. 45, n. 4, p. 205-211, 2003.1678-9946https://www.arca.fiocruz.br/handle/icict/3316910.1590/S0036-46652003000400006engMcCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replicationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Adolfo Lutz. São Paulo, SP, Brasil.Instituto Adolfo Lutz. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Instituto Adolfo Lutz. São Paulo, SP, Brasil.Fundação Pró-Sangue/Hemocentro. São Paulo, SP, Brasil.Several studies have recently shown the use of recombinant rabies virus as potential vector-viral vaccine for HIV-1. The sequence homology between gp 120 and rabies virus glycoprotein has been reported. The McCoy cell line has therefore been used to show CD4+ or CD4+ like receptors. Samples of HIV-1 were isolated, when plasma of HIV-1 positive patients was inoculated in the McCoy cell line. The virus infection was then studied during successive virus passages. The proteins released in the extra cellular medium were checked for protein activity, by exposure to SDS Electrophoresis and blotting to nitro-cellulose filter, then reacting with sera of HIV positive and negative patients. Successive passages were performed, and showed viral replication, membrane permeabilization, the syncytium formation, and the cellular lysis (cytopathic effect). Flow cytometry analysis shows clear evidence that CD4+ receptors are present in this cell line, which enhances the likelihood of easy isolation and replication of HIV. The results observed allow the use of this cell line as a possible model for isolating HIV, as well as for carrying out studies of the dynamics of viral infection in several situations, including exposure to drugs in pharmacological studies, and possibly studies and analyses of the immune response in vaccine therapies.HIV-1 Rabies virusViral ReplicationRabies virusCD4+McCoy cell lineinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33169/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINAL17077.pdf17077.pdfapplication/pdf609724https://www.arca.fiocruz.br/bitstream/icict/33169/2/17077.pdf237e4e4abefe2dd969303a61debf8ef5MD52TEXT17077.pdf.txt17077.pdf.txtExtracted texttext/plain36089https://www.arca.fiocruz.br/bitstream/icict/33169/3/17077.pdf.txt61d0295f9bd85bfa36d9ddb485785abeMD53icict/331692019-05-26 13:08:56.85oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-05-26T16:08:56Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
title McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
spellingShingle McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
Nogueira, Yeda L.
HIV-1 Rabies virus
Viral Replication
Rabies virus
CD4+
McCoy cell line
title_short McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
title_full McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
title_fullStr McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
title_full_unstemmed McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
title_sort McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
author Nogueira, Yeda L.
author_facet Nogueira, Yeda L.
Oliveira, Carmen A. Freitas
Ferreira, Antonio Gomes P.
Nakamura, Paulo M.
Magnanelli, Antonio Carlos
author_role author
author2 Oliveira, Carmen A. Freitas
Ferreira, Antonio Gomes P.
Nakamura, Paulo M.
Magnanelli, Antonio Carlos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Nogueira, Yeda L.
Oliveira, Carmen A. Freitas
Ferreira, Antonio Gomes P.
Nakamura, Paulo M.
Magnanelli, Antonio Carlos
dc.subject.en.pt_BR.fl_str_mv HIV-1 Rabies virus
Viral Replication
Rabies virus
CD4+
McCoy cell line
topic HIV-1 Rabies virus
Viral Replication
Rabies virus
CD4+
McCoy cell line
description Instituto Adolfo Lutz. São Paulo, SP, Brasil.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2019-05-22T12:59:50Z
dc.date.available.fl_str_mv 2019-05-22T12:59:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NOGUEIRA, Yeda L. et al . McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication. Rev. Inst. Med. Trop. S. Paulo, São Paulo, v. 45, n. 4, p. 205-211, 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33169
dc.identifier.issn.pt_BR.fl_str_mv 1678-9946
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/S0036-46652003000400006
identifier_str_mv NOGUEIRA, Yeda L. et al . McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication. Rev. Inst. Med. Trop. S. Paulo, São Paulo, v. 45, n. 4, p. 205-211, 2003.
1678-9946
10.1590/S0036-46652003000400006
url https://www.arca.fiocruz.br/handle/icict/33169
dc.language.iso.fl_str_mv eng
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