Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies

Detalhes bibliográficos
Autor(a) principal: Franco, Antonia Maria Ramos
Data de Publicação: 1997
Outros Autores: Machado, Gérzia M. C., Naiff, Roberto D., Moreira, Celia F. S., McMahon-Pratt, Diane, Grimaldi Jr., Gabriel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43843
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
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spelling Franco, Antonia Maria RamosMachado, Gérzia M. C.Naiff, Roberto D.Moreira, Celia F. S.McMahon-Pratt, DianeGrimaldi Jr., Gabriel2020-10-07T12:57:12Z2020-10-07T12:57:12Z1997FRANCO, Antonia Maria Ramos et al. Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 1, p. 63-69, Jan./Feb. 1997.0074-0276https://www.arca.fiocruz.br/handle/icict/4384310.1590/S0074-027619970001000131678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.EndotrypanumKinetoplastidTrypanosomatidaeReservatórios de mamíferosCaracterização molecularAnticorpos monoclonaisEndotrypanumKinetoplastidTrypanosomatidaeMammalian reservoirsMolecular characterizationMonoclonal antibodiesCharacterization of Endotrypanum Parasites Using Specific Monoclonal Antibodiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Pesquisas da Amazônia. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.DEPH. Yale University School of Medicine. New Haven, CT, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.A large number of Endotrypanum stocks (representing an heterogeneous population of strains) have been screened against a panel of monoclonal antibodies (MAbs) derived for selected species of Endotrypanum or Leishmania, to see whether this approach could be used to group/differentiate further among these parasites. Using different immunological assay systems, MAbs considered specific for the genus Endotrypanum (E-24, CXXX-3G5-F12) or strain M6159 of E. schaudinni (E-2, CXIV-3C7-F5) reacted variably according to the test used but in the ELISA or immunofluorescence assay both reacted with all the strains tested. Analyses using these MAbs showed antigenic diversity occurring among the Endotrypanum strains, but no qualitative or quantitative reactivity pattern could be consistently related to parasite origin (i.e., host species involved) or geographic area of isolation. Western blot analyses of the parasites showed that these MAbs recognized multiple components. Differences existed either in the epitope density or molecular forms associated with the antigenic determinants and therefore allowed the assignment of the strains to specific antigenic groups. Using immunofluorescence or ELISA assay, clone E-24 produced reaction with L. equatorensis (which is a parasite of sloth and rodent), but not with other trypanosomatids examined. Interestingly, the latter parasite and the Endotrypanum strains crossreacted with a number of MAbs that were produced against members of the L. major-L. tropica complex.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43843/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGerziaMachado_AntoniaFranco_etal_IOC_1997.pdfGerziaMachado_AntoniaFranco_etal_IOC_1997.pdfapplication/pdf1043779https://www.arca.fiocruz.br/bitstream/icict/43843/2/GerziaMachado_AntoniaFranco_etal_IOC_1997.pdf0bd035f5c1df4b0be3a63e4d1bb6edf0MD52TEXTGerziaMachado_AntoniaFranco_etal_IOC_1997.pdf.txtGerziaMachado_AntoniaFranco_etal_IOC_1997.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
title Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
spellingShingle Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
Franco, Antonia Maria Ramos
Endotrypanum
Kinetoplastid
Trypanosomatidae
Reservatórios de mamíferos
Caracterização molecular
Anticorpos monoclonais
Endotrypanum
Kinetoplastid
Trypanosomatidae
Mammalian reservoirs
Molecular characterization
Monoclonal antibodies
title_short Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
title_full Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
title_fullStr Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
title_full_unstemmed Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
title_sort Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
author Franco, Antonia Maria Ramos
author_facet Franco, Antonia Maria Ramos
Machado, Gérzia M. C.
Naiff, Roberto D.
Moreira, Celia F. S.
McMahon-Pratt, Diane
Grimaldi Jr., Gabriel
author_role author
author2 Machado, Gérzia M. C.
Naiff, Roberto D.
Moreira, Celia F. S.
McMahon-Pratt, Diane
Grimaldi Jr., Gabriel
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Franco, Antonia Maria Ramos
Machado, Gérzia M. C.
Naiff, Roberto D.
Moreira, Celia F. S.
McMahon-Pratt, Diane
Grimaldi Jr., Gabriel
dc.subject.other.pt_BR.fl_str_mv Endotrypanum
Kinetoplastid
Trypanosomatidae
Reservatórios de mamíferos
Caracterização molecular
Anticorpos monoclonais
topic Endotrypanum
Kinetoplastid
Trypanosomatidae
Reservatórios de mamíferos
Caracterização molecular
Anticorpos monoclonais
Endotrypanum
Kinetoplastid
Trypanosomatidae
Mammalian reservoirs
Molecular characterization
Monoclonal antibodies
dc.subject.en.pt_BR.fl_str_mv Endotrypanum
Kinetoplastid
Trypanosomatidae
Mammalian reservoirs
Molecular characterization
Monoclonal antibodies
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.
publishDate 1997
dc.date.issued.fl_str_mv 1997
dc.date.accessioned.fl_str_mv 2020-10-07T12:57:12Z
dc.date.available.fl_str_mv 2020-10-07T12:57:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRANCO, Antonia Maria Ramos et al. Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 1, p. 63-69, Jan./Feb. 1997.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43843
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761997000100013
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv FRANCO, Antonia Maria Ramos et al. Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 1, p. 63-69, Jan./Feb. 1997.
0074-0276
10.1590/S0074-02761997000100013
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43843
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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