Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva

Detalhes bibliográficos
Autor(a) principal: Cuervo, Patrícia
Data de Publicação: 2008
Outros Autores: Rodrigues, Camila Mesquita, d`Avila-Levy, C. M., Brito, Constança, Pires, Fabiano Araújo, Gredilha, Rodrigo, Alves, Carlos Roberto, Jesus, José Batista de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32574
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Leishmanioses. Rio de Janeiro, RJ. Brasil.
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spelling Cuervo, PatríciaRodrigues, Camila Mesquitad`Avila-Levy, C. M.Brito, ConstançaPires, Fabiano AraújoGredilha, RodrigoAlves, Carlos RobertoJesus, José Batista de2019-04-18T12:48:43Z2019-04-18T12:48:43Z2008CUERVO, Patricia et al. Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 5, p. 504-506, Aug. 2008.0074-0276https://www.arca.fiocruz.br/handle/icict/3257410.1590/S0074-027620080005000181678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzSarcofagídeosProteases de serina semelhantes a quimotripsinaOxysarcodexia thornaxOxysarcodexia thornaxSarcophagidaeChymotrypsin-like serine proteasesSerine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larvainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Leishmanioses. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Diptera. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.We report for the first time the expression of multiple protease activities in the first instar larva (L1) of the flesh fly Oxysarcodexia thornax (Walker). Zymographic analysis of homogenates from freshly obtained L1 revealed a complex proteolytic profile ranging from 21.5 to 136 kDa. Although some activities were detected at pH 3.5 and 5.5, the optimum pH for most of the proteolytic activities was between pH 7.5 and 9.5. Seven of 10 proteases were completely inactivated by phenyl-methyl sulfonyl-fluoride, suggesting that main proteases expressed by L1 belong to serine proteases class. Complete inactivation of all enzymatic activities was obtained using N-p-Tosyl-L-phenylalanine chloromethyl ketone (100 µM), a specific inhibitor of chymotrypsin-like serine proteases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/32574/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPatriciaCuervo_CamilaRodrigues_etal_IOC_2008.pdfPatriciaCuervo_CamilaRodrigues_etal_IOC_2008.pdfapplication/pdf364061https://www.arca.fiocruz.br/bitstream/icict/32574/2/PatriciaCuervo_CamilaRodrigues_etal_IOC_2008.pdf865baab9254dd68be37e6b8e3ee67fb2MD52TEXTPatriciaCuervo_CamilaRodrigues_etal_IOC_2008.pdf.txtPatriciaCuervo_CamilaRodrigues_etal_IOC_2008.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva
title Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva
spellingShingle Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva
Cuervo, Patrícia
Sarcofagídeos
Proteases de serina semelhantes a quimotripsina
Oxysarcodexia thornax
Oxysarcodexia thornax
Sarcophagidae
Chymotrypsin-like serine proteases
title_short Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva
title_full Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva
title_fullStr Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva
title_full_unstemmed Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva
title_sort Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva
author Cuervo, Patrícia
author_facet Cuervo, Patrícia
Rodrigues, Camila Mesquita
d`Avila-Levy, C. M.
Brito, Constança
Pires, Fabiano Araújo
Gredilha, Rodrigo
Alves, Carlos Roberto
Jesus, José Batista de
author_role author
author2 Rodrigues, Camila Mesquita
d`Avila-Levy, C. M.
Brito, Constança
Pires, Fabiano Araújo
Gredilha, Rodrigo
Alves, Carlos Roberto
Jesus, José Batista de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cuervo, Patrícia
Rodrigues, Camila Mesquita
d`Avila-Levy, C. M.
Brito, Constança
Pires, Fabiano Araújo
Gredilha, Rodrigo
Alves, Carlos Roberto
Jesus, José Batista de
dc.subject.other.pt_BR.fl_str_mv Sarcofagídeos
Proteases de serina semelhantes a quimotripsina
Oxysarcodexia thornax
topic Sarcofagídeos
Proteases de serina semelhantes a quimotripsina
Oxysarcodexia thornax
Oxysarcodexia thornax
Sarcophagidae
Chymotrypsin-like serine proteases
dc.subject.en.pt_BR.fl_str_mv Oxysarcodexia thornax
Sarcophagidae
Chymotrypsin-like serine proteases
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas em Leishmanioses. Rio de Janeiro, RJ. Brasil.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2019-04-18T12:48:43Z
dc.date.available.fl_str_mv 2019-04-18T12:48:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CUERVO, Patricia et al. Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 5, p. 504-506, Aug. 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32574
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/S0074-02762008000500018
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv CUERVO, Patricia et al. Serine protease activities in Oxysarcodexia thornax (Walker) (Diptera: Sarcophagidae) first instar larva. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 5, p. 504-506, Aug. 2008.
0074-0276
10.1590/S0074-02762008000500018
1678-8060
url https://www.arca.fiocruz.br/handle/icict/32574
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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