Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/29682 |
Resumo: | Gonçalo Moniz Institute, Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (Proc. 404242/2012-0) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco-FACEPE (Proc. APQ-1257-2.11/12). W. V. Sousa and M. A. Krieger are research fellows of CNPq Proc. No. 306222/2013-2 and 590032/2011-9, respectively. |
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Santos, Fred Luciano NevesCampos, Ana Clara PaixãoAmorim, Leila Denise Alves FerreiraSilva, Edimilson Domingos daZanchin, Nilson Ivo ToninCeledon, Paola Alejandra FioraniDel-Rei, Rodrigo PimentaKrieger, Marco AurélioGomes, Yara de Miranda2018-10-23T12:34:46Z2018-10-23T12:34:46Z2018SANTOS, F. L. N. et al. Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis. American Journal of Tropical Medicine and Hygiene, p. 1-6, 2018.0002-9637https://www.arca.fiocruz.br/handle/icict/29682Gonçalo Moniz Institute, Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (Proc. 404242/2012-0) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco-FACEPE (Proc. APQ-1257-2.11/12). W. V. Sousa and M. A. Krieger are research fellows of CNPq Proc. No. 306222/2013-2 and 590032/2011-9, respectively.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Fio-Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Molecular Biology Institute of Paraná. Curitiba, PR, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Fio-Chagas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Molecular Biology Institute of Paraná. Curitiba, PR, Brazil.Fundação Oswaldo Cruz. Fio-Chagas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.The existence of an imperfect reference standard presents complications when evaluating the unbiased performance of novel diagnostic techniques. This is especially true in the absence of a gold standard, as is the case in chronic Chagas disease (CD) diagnosis. To circumvent this constraint, we elected to use latent class analysis (LCA). Previously, our group demonstrated the high performance of four Trypanosoma cruzi–chimeric proteins (Molecular Biology Institute of Parana´ [IBMP]-8.1, -8.2, -8.3, and -8.4) for CD diagnosis using several distinct immunoassays. Although commercial tests had previously been established as a reference standard, the diagnostic performance of these chimeric antigens could present bias because these tests fail to produce 100% accurate results. Thus, we used LCA to assess the performance of these IBMP chimeric antigens in chronic CD diagnosis. Using the LCA model as a gold standard, sensitivity and specificity values ranged from 93.5% to 99.4% and 99.6% to 100%, respectively. The accuracy values were 96.2% for IBMP-8.2, approximately 98% for IBMP-8.1 and IBMP-8.3, and nearly 100% for IBMP-8.4. For IBMP-8.1 and IBMP-8.2, higher positive predictive values were associated with increases in hypothetical prevalence. Similarly, higher hypothetical prevalence resulted in lower negative predictive values for IBMP-8.1, IBMP-8.2, and IBMP- 8.3. In addition, samples with serodiscordant results from commercial serological tests were analyzed using LCA. Molecular Biology Institute of Parana´ -8.1 demonstrated potential for use in confirmatory testing with regard to samples with inconsistent results. Moreover, our findings further confirmed the remarkable performance of the IBMP-8.4 antigen to diagnose chronic CD in both endemic and non-endemic areas.engAmerican Society of Tropical Medicine and HygieneDoença de ChagasDiagnósticoTrypanosoma cruziProteínas QuiméricasChagas diseaseDiagnosisTrypanosoma cruziChimeric ProteinsHighly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/29682/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos FL Highly Accurate Chimeric...2018.pdfSantos FL Highly Accurate 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| dc.title.pt_BR.fl_str_mv |
Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis |
| title |
Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis |
| spellingShingle |
Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis Santos, Fred Luciano Neves Doença de Chagas Diagnóstico Trypanosoma cruzi Proteínas Quiméricas Chagas disease Diagnosis Trypanosoma cruzi Chimeric Proteins |
| title_short |
Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis |
| title_full |
Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis |
| title_fullStr |
Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis |
| title_full_unstemmed |
Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis |
| title_sort |
Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis |
| author |
Santos, Fred Luciano Neves |
| author_facet |
Santos, Fred Luciano Neves Campos, Ana Clara Paixão Amorim, Leila Denise Alves Ferreira Silva, Edimilson Domingos da Zanchin, Nilson Ivo Tonin Celedon, Paola Alejandra Fiorani Del-Rei, Rodrigo Pimenta Krieger, Marco Aurélio Gomes, Yara de Miranda |
| author_role |
author |
| author2 |
Campos, Ana Clara Paixão Amorim, Leila Denise Alves Ferreira Silva, Edimilson Domingos da Zanchin, Nilson Ivo Tonin Celedon, Paola Alejandra Fiorani Del-Rei, Rodrigo Pimenta Krieger, Marco Aurélio Gomes, Yara de Miranda |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Fred Luciano Neves Campos, Ana Clara Paixão Amorim, Leila Denise Alves Ferreira Silva, Edimilson Domingos da Zanchin, Nilson Ivo Tonin Celedon, Paola Alejandra Fiorani Del-Rei, Rodrigo Pimenta Krieger, Marco Aurélio Gomes, Yara de Miranda |
| dc.subject.other.pt_BR.fl_str_mv |
Doença de Chagas Diagnóstico Trypanosoma cruzi Proteínas Quiméricas |
| topic |
Doença de Chagas Diagnóstico Trypanosoma cruzi Proteínas Quiméricas Chagas disease Diagnosis Trypanosoma cruzi Chimeric Proteins |
| dc.subject.en.pt_BR.fl_str_mv |
Chagas disease Diagnosis Trypanosoma cruzi Chimeric Proteins |
| description |
Gonçalo Moniz Institute, Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (Proc. 404242/2012-0) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco-FACEPE (Proc. APQ-1257-2.11/12). W. V. Sousa and M. A. Krieger are research fellows of CNPq Proc. No. 306222/2013-2 and 590032/2011-9, respectively. |
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2018 |
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2018-10-23T12:34:46Z |
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2018-10-23T12:34:46Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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publishedVersion |
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SANTOS, F. L. N. et al. Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis. American Journal of Tropical Medicine and Hygiene, p. 1-6, 2018. |
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https://www.arca.fiocruz.br/handle/icict/29682 |
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0002-9637 |
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SANTOS, F. L. N. et al. Highly Accurate Chimeric Proteins for the Serological Diagnosis of Chronic Chagas Disease: A Latent Class Analysis. American Journal of Tropical Medicine and Hygiene, p. 1-6, 2018. 0002-9637 |
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American Society of Tropical Medicine and Hygiene |
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