The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells

Detalhes bibliográficos
Autor(a) principal: Robert, Anny Waloski
Data de Publicação: 2019
Outros Autores: Gomes, Felipe Azevedo, Rode, Michele Patricia, Silva, Maiara Marques da, Veleirinho, Maria Beatriz da Rocha, Maraschin, Marcelo, Hayashi, Leila, Calloni, Giordano Wosgrau, Stimamiglio, Marco Augusto
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34110
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
id CRUZ_d248a66052eeb7bc1fef69d702422521
oai_identifier_str oai:www.arca.fiocruz.br:icict/34110
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Robert, Anny WaloskiGomes, Felipe AzevedoRode, Michele PatriciaSilva, Maiara Marques daVeleirinho, Maria Beatriz da RochaMaraschin, MarceloHayashi, LeilaCalloni, Giordano WosgrauStimamiglio, Marco Augusto2019-07-15T17:40:34Z2019-07-15T17:40:34Z2019ROBERT, Anny Waloski et al. The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells. Journal of Tissue Engineering, v. 10, p. 1-10, 2019.2041-7314https://www.arca.fiocruz.br/handle/icict/3411010.1177/20417314198333912041-7314porSAGE PublicationsSecretomeStromal CellsWound HealingPhysiologic AngiogenesisCélulas del EstromaCicatrización de HeridasAngiogénesis fisiológicaCélulas EstromaisCicatrizaçãoAngiogênese FisiológicaThe skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil.Universidade Federal de Santa Catarina. Florianópolis, SC, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Multipotent stromal cells stimulate skin regeneration after acute or chronic injuries. However, many stem cell therapy protocols are limited by the elevated number of cells required and poor cell survival after transplantation. Considering that the beneficial effects of multipotent stromal cells on wound healing are typically mediated by paracrine mechanisms, we examined whether the conditioned medium from skin-derived multipotent stromal cells would be beneficial for restoring the skin structure of mice after wounding. A proteomic characterization of skin-derived multipotent stromal cell-conditioned medium was performed, and the angiogenic function of this secretome was investigated in vitro using an endothelial cell tube formation assay. We then applied the skin-derived multipotent stromal cell-conditioned medium directly to full-thickness excisional wounds or embedded it into carrageenan or poly(vinyl alcohol) hydrogels to monitor tissue regeneration in mice. Biological processes related to wound healing and angiogenesis were highlighted by the analysis of the skin-derived multipotent stromal cell secretome, and a pro-angiogenic capacity for promoting tubule-like structures was first confirmed in vitro. Skin wounds treated with skin-derived multipotent stromal cell-conditioned medium also displayed increased angiogenesis, independently of the association of the conditioned medium with hydrogels. However, improvements in wound closure and epidermis or decreased inflammatory cell presence were not observed. Hence, the use of the secretome obtained from human skin-derived multipotent stromal cells may be a potential strategy to aid the natural skin repair of full-thickness lesions mainly based on its pro-angiogenic properties.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/34110/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL20417314198333.pdf20417314198333.pdfapplication/pdf663092https://www.arca.fiocruz.br/bitstream/icict/34110/2/20417314198333.pdf7e5d4a2eaa46b5e6b3536d90fe950bc3MD52TEXT20417314198333.pdf.txt20417314198333.pdf.txtExtracted texttext/plain44288https://www.arca.fiocruz.br/bitstream/icict/34110/3/20417314198333.pdf.txt17fd7af3538aac936703eab74b172f84MD53icict/341102019-07-16 02:02:58.879oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-07-16T05:02:58Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells
title The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells
spellingShingle The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells
Robert, Anny Waloski
Secretome
Stromal Cells
Wound Healing
Physiologic Angiogenesis
Células del Estroma
Cicatrización de Heridas
Angiogénesis fisiológica
Células Estromais
Cicatrização
Angiogênese Fisiológica
title_short The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells
title_full The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells
title_fullStr The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells
title_full_unstemmed The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells
title_sort The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells
author Robert, Anny Waloski
author_facet Robert, Anny Waloski
Gomes, Felipe Azevedo
Rode, Michele Patricia
Silva, Maiara Marques da
Veleirinho, Maria Beatriz da Rocha
Maraschin, Marcelo
Hayashi, Leila
Calloni, Giordano Wosgrau
Stimamiglio, Marco Augusto
author_role author
author2 Gomes, Felipe Azevedo
Rode, Michele Patricia
Silva, Maiara Marques da
Veleirinho, Maria Beatriz da Rocha
Maraschin, Marcelo
Hayashi, Leila
Calloni, Giordano Wosgrau
Stimamiglio, Marco Augusto
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Robert, Anny Waloski
Gomes, Felipe Azevedo
Rode, Michele Patricia
Silva, Maiara Marques da
Veleirinho, Maria Beatriz da Rocha
Maraschin, Marcelo
Hayashi, Leila
Calloni, Giordano Wosgrau
Stimamiglio, Marco Augusto
dc.subject.other.pt_BR.fl_str_mv Secretome
topic Secretome
Stromal Cells
Wound Healing
Physiologic Angiogenesis
Células del Estroma
Cicatrización de Heridas
Angiogénesis fisiológica
Células Estromais
Cicatrização
Angiogênese Fisiológica
dc.subject.en.pt_BR.fl_str_mv Stromal Cells
Wound Healing
Physiologic Angiogenesis
dc.subject.es.pt_BR.fl_str_mv Células del Estroma
Cicatrización de Heridas
Angiogénesis fisiológica
dc.subject.decs.pt_BR.fl_str_mv Células Estromais
Cicatrização
Angiogênese Fisiológica
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-07-15T17:40:34Z
dc.date.available.fl_str_mv 2019-07-15T17:40:34Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROBERT, Anny Waloski et al. The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells. Journal of Tissue Engineering, v. 10, p. 1-10, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34110
dc.identifier.issn.pt_BR.fl_str_mv 2041-7314
dc.identifier.doi.none.fl_str_mv 10.1177/2041731419833391
dc.identifier.eissn.none.fl_str_mv 2041-7314
identifier_str_mv ROBERT, Anny Waloski et al. The skin regeneration potential of a pro-angiogenic secretome from human skin-derived multipotent stromal cells. Journal of Tissue Engineering, v. 10, p. 1-10, 2019.
2041-7314
10.1177/2041731419833391
url https://www.arca.fiocruz.br/handle/icict/34110
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/34110/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/34110/2/20417314198333.pdf
https://www.arca.fiocruz.br/bitstream/icict/34110/3/20417314198333.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
7e5d4a2eaa46b5e6b3536d90fe950bc3
17fd7af3538aac936703eab74b172f84
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324768135446528

Registros relacionados