DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59605 |
Resumo: | Helminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. |
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Araújo, Amanda Domingues deCarvalho, Omar Dos SantosGava, Sandra GrossiCaldeira, Roberta Lima2023-07-14T18:24:06Z2023-07-14T18:24:06Z2023ARAÚJO, Amanda Domingues de et al. DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). Frontiers in cellular and infection microbiology, v. 13, 1167787, 2023. doi.org/10.3389/fcimb.2023.11677872235-2988https://www.arca.fiocruz.br/handle/icict/59605engFrontiers Media SADNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae).info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHelminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Helminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Helminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Helminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Introduction: The genus Biomphalaria in Brazil includes 11 species and one subspecies, three of which are intermediate hosts of Schistosoma mansoni. Due to the recent evolution of this group, some species are difficult to identify based on morphological characters, making the use of genetic markers necessary for species identification. This study aimed to evaluate the use of partial sequences of the cytochrome c oxidase I (coi) gene for the identification of Biomphalaria species using phylogenetic reconstruction and species delimitation algorithms. The study tested the use of DNA barcoding technique for species delimitation within the genus. Methods: DNA barcoding was performed by sequencing a partial region of the coi gene from specimens, and the sequences were analyzed using phylogenetic reconstruction and algorithms to delimit Operational Taxonomic Units (OTUs). Results: The study found that the use of the coi gene in the reconstruction of the phylogeny of the genus might be an alternative for understanding the evolution and dispersion of species. However, this marker alone is not enough to solve complex taxonomic problems within the genus. A total of 223 sequences were analyzed, 102 of which could be separated using the barcode gap, enabling the correct identification of seven taxa. Discussion: The study demonstrated that accurate mollusk identification is necessary for effective schistosomiasis control. The DNA barcoding methodology was found to be promising for accurate mollusk identification, which is crucial for concentrating schistosomiasis control efforts in places where it is needed.DNA barcodemollusksBiomphalariamedical malacology collectionmolecular identificationcytochrome c oxidase coiinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/59605/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda_ Planorbidae).pdfDNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda_ Planorbidae).pdfapplication/pdf6740304https://www.arca.fiocruz.br/bitstream/icict/59605/2/DNA%20barcoding%20as%20a%20valuable%20tool%20for%20delimiting%20mollusk%20species%20of%20the%20genus%20Biomphalaria%20Preston%2c%201910%20%28Gastropoda_%20Planorbidae%29.pdfd57f015373cd0df0c6a31f4a0bdcdcd5MD52icict/596052023-07-14 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dc.title.en_US.fl_str_mv |
DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). |
title |
DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). |
spellingShingle |
DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). Araújo, Amanda Domingues de DNA barcode mollusks Biomphalaria medical malacology collection molecular identification cytochrome c oxidase coi |
title_short |
DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). |
title_full |
DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). |
title_fullStr |
DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). |
title_full_unstemmed |
DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). |
title_sort |
DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). |
author |
Araújo, Amanda Domingues de |
author_facet |
Araújo, Amanda Domingues de Carvalho, Omar Dos Santos Gava, Sandra Grossi Caldeira, Roberta Lima |
author_role |
author |
author2 |
Carvalho, Omar Dos Santos Gava, Sandra Grossi Caldeira, Roberta Lima |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Araújo, Amanda Domingues de Carvalho, Omar Dos Santos Gava, Sandra Grossi Caldeira, Roberta Lima |
dc.subject.en.en_US.fl_str_mv |
DNA barcode mollusks Biomphalaria medical malacology collection molecular identification cytochrome c oxidase coi |
topic |
DNA barcode mollusks Biomphalaria medical malacology collection molecular identification cytochrome c oxidase coi |
description |
Helminthology and Medical Malacology Research Group. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-07-14T18:24:06Z |
dc.date.available.fl_str_mv |
2023-07-14T18:24:06Z |
dc.date.issued.fl_str_mv |
2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARAÚJO, Amanda Domingues de et al. DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). Frontiers in cellular and infection microbiology, v. 13, 1167787, 2023. doi.org/10.3389/fcimb.2023.1167787 |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/59605 |
dc.identifier.issn.en_US.fl_str_mv |
2235-2988 |
identifier_str_mv |
ARAÚJO, Amanda Domingues de et al. DNA barcoding as a valuable tool for delimiting mollusk species of the genus Biomphalaria Preston, 1910 (Gastropoda: Planorbidae). Frontiers in cellular and infection microbiology, v. 13, 1167787, 2023. doi.org/10.3389/fcimb.2023.1167787 2235-2988 |
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https://www.arca.fiocruz.br/handle/icict/59605 |
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eng |
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eng |
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openAccess |
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Frontiers Media SA |
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Frontiers Media SA |
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