Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15972 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Brasil. |
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Oliveira, Jose Henrique M.Gonçalves, Renata L. S.Lara, Flavio A.Dias, Felipe A.Gandara, Ana Caroline P.Menna-Barreto, Rubem F. S.Edwards, Meredith C.Laurindo, Francisco R. M.Silva-Neto, Mário A. C.Sorgine, Marcos H. F.Oliveira, Pedro L.2016-09-27T16:23:50Z2016-09-27T16:23:50Z2011OLIVEIRA, Jose Henrique M. et al. Blood Meal-Derived Heme Decreases ROS Levels in the Midgut of Aedes aegypti and Allows Proliferation of Intestinal Microbiota. Plos One, v.7, n.3, e1001320, 14p, Mar. 2011.1553-7366https://www.arca.fiocruz.br/handle/icict/1597210.1371/journal.ppat.10013201553-7374engPublic Library of ScienceAedes aegyptiMicrobiotaEspécies de Oxigênio ReativasHemeIntestinoAedes aegyptiIntestinal MicrobiotaReactive oxygen speciesBlood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiotainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Instituto de Bioquímica Médica. Laboratório de Bioquímica Redox. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Escola de Medicina. Instituto do Coração (INCOR). Laboratório de Biologia Vascular. São Paulo, SP, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica, Programa de Biologia Molecular e Biotecnologia. Laboratório de Sinalização Celular. Rio de janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Brasil..Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Brasil.The presence of bacteria in the midgut of mosquitoes antagonizes infectious agents, such as Dengue and Plasmodium, acting as a negative factor in the vectorial competence of the mosquito. Therefore, knowledge of the molecular mechanisms involved in the control of midgut microbiota could help in the development of new tools to reduce transmission. We hypothesized that toxic reactive oxygen species (ROS) generated by epithelial cells control bacterial growth in the midgut of Aedes aegypti, the vector of Yellow fever and Dengue viruses. We show that ROS are continuously present in the midgut of sugar-fed (SF) mosquitoes and a blood-meal immediately decreased ROS through a mechanism involving heme-mediated activation of PKC. This event occurred in parallel with an expansion of gut bacteria. Treatment of sugar-fed mosquitoes with increased concentrations of heme led to a dose dependent decrease in ROS levels and a consequent increase in midgut endogenous bacteria. In addition, gene silencing of dual oxidase (Duox) reduced ROS levels and also increased gut flora. Using a model of bacterial oral infection in the gut, we show that the absence of ROS resulted in decreased mosquito resistance to infection, increased midgut epithelial damage, transcriptional modulation of immune-related genes and mortality. As heme is a pro-oxidant molecule released in large amounts upon hemoglobin degradation, oxidative killing of bacteria in the gut would represent a burden to the insect, thereby creating an extra oxidative challenge to the mosquito. We propose that a controlled decrease in ROS levels in the midgut of Aedes aegypti is an adaptation to compensate for the ingestion of heme.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15972/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALflavio_lara_etal_IOC_2011.pdfflavio_lara_etal_IOC_2011.pdfapplication/pdf5764433https://www.arca.fiocruz.br/bitstream/icict/15972/2/flavio_lara_etal_IOC_2011.pdf026e60697d03e0b239a5be552cef7922MD52TEXTflavio_lara_etal_IOC_2011.pdf.txtflavio_lara_etal_IOC_2011.pdf.txtExtracted texttext/plain68766https://www.arca.fiocruz.br/bitstream/icict/15972/3/flavio_lara_etal_IOC_2011.pdf.txtbfa5b6df33a9d5ea81057bcd95e6af48MD53icict/159722018-04-02 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| dc.title.pt_BR.fl_str_mv |
Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota |
| title |
Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota |
| spellingShingle |
Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota Oliveira, Jose Henrique M. Aedes aegypti Microbiota Espécies de Oxigênio Reativas Heme Intestino Aedes aegypti Intestinal Microbiota Reactive oxygen species |
| title_short |
Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota |
| title_full |
Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota |
| title_fullStr |
Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota |
| title_full_unstemmed |
Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota |
| title_sort |
Blood meal-derived heme decreases ROS levels in the midgut of Aedes aegypti and allows proliferation of intestinal microbiota |
| author |
Oliveira, Jose Henrique M. |
| author_facet |
Oliveira, Jose Henrique M. Gonçalves, Renata L. S. Lara, Flavio A. Dias, Felipe A. Gandara, Ana Caroline P. Menna-Barreto, Rubem F. S. Edwards, Meredith C. Laurindo, Francisco R. M. Silva-Neto, Mário A. C. Sorgine, Marcos H. F. Oliveira, Pedro L. |
| author_role |
author |
| author2 |
Gonçalves, Renata L. S. Lara, Flavio A. Dias, Felipe A. Gandara, Ana Caroline P. Menna-Barreto, Rubem F. S. Edwards, Meredith C. Laurindo, Francisco R. M. Silva-Neto, Mário A. C. Sorgine, Marcos H. F. Oliveira, Pedro L. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Jose Henrique M. Gonçalves, Renata L. S. Lara, Flavio A. Dias, Felipe A. Gandara, Ana Caroline P. Menna-Barreto, Rubem F. S. Edwards, Meredith C. Laurindo, Francisco R. M. Silva-Neto, Mário A. C. Sorgine, Marcos H. F. Oliveira, Pedro L. |
| dc.subject.other.pt_BR.fl_str_mv |
Aedes aegypti Microbiota Espécies de Oxigênio Reativas Heme Intestino |
| topic |
Aedes aegypti Microbiota Espécies de Oxigênio Reativas Heme Intestino Aedes aegypti Intestinal Microbiota Reactive oxygen species |
| dc.subject.en.pt_BR.fl_str_mv |
Aedes aegypti Intestinal Microbiota Reactive oxygen species |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Laboratório de Bioquímica de Artrópodes Hematófagos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM), Brasil. |
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2011 |
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2011 |
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2016-09-27T16:23:50Z |
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2016-09-27T16:23:50Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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OLIVEIRA, Jose Henrique M. et al. Blood Meal-Derived Heme Decreases ROS Levels in the Midgut of Aedes aegypti and Allows Proliferation of Intestinal Microbiota. Plos One, v.7, n.3, e1001320, 14p, Mar. 2011. |
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https://www.arca.fiocruz.br/handle/icict/15972 |
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1553-7366 |
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10.1371/journal.ppat.1001320 |
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1553-7374 |
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OLIVEIRA, Jose Henrique M. et al. Blood Meal-Derived Heme Decreases ROS Levels in the Midgut of Aedes aegypti and Allows Proliferation of Intestinal Microbiota. Plos One, v.7, n.3, e1001320, 14p, Mar. 2011. 1553-7366 10.1371/journal.ppat.1001320 1553-7374 |
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Public Library of Science |
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Public Library of Science |
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