A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/55682 |
Resumo: | Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. |
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Paiva, Luciene Pimenta deReis, Jordana Grazziela Alves Coelho DosTrindade, Bruno CaetanoPascoal, Vanessa Peruhype MagalhãesAraújo, Márcio Sobreira SilvaGonçalves, Juan JonathanNogueira, Ana CarolineMartins, Júlia PereiraRibeiro, Ágata LopesStarling, Ana LuciaAlcântara, Luiz Carlos JúniorRibeiro, Maísa AparecidaProietti, Anna Bárbara de Freitas CarneiroSabino, Ester CerdeiraBicalho, Kelly AlvesCarvalho, Andréa TeixeiraMartins Filho, Olindo Assis2022-11-17T17:43:10Z2022-11-17T17:43:10Z2022PAIVA, Luciene Pimenta de et al. A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection. Frontiers in Immunology, v. 13, 795815, p. 1-15, Apr. 2022.1664-3224https://www.arca.fiocruz.br/handle/icict/5568210.3389/fimmu.2022.795815engFrontiers Research FoundationA New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Estado de Minas Gerais. Fundação Centro de Hematologia e Hemoterapia. Belo Horizonte, MG, Brazil.Estado de Minas Gerais. Fundação Centro de Hematologia e Hemoterapia. Belo Horizonte, MG, Brazil.Universidade de São Paulo. Ribeirão Preto, SP, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.In the present work, we developed and evaluated the performance of a new flow cytometry-based single platform, referred to as "FC-Duplex IgG1 (HTLV-1/2)", for universal and differential serodiagnosis of HTLV-1/2 infection. The proposed technology employs a system for detection of IgG1 antibodies in a single competitive immunofluorescence platform by flow cytometry using fluorescently labeled MT-2/MoT cell line mix coupled to a highly sensitive development system (Biotin/Streptavidin/Phycoerythrin). The stability of fluorescent labeling and the antigenicity of MT-2 and MoT cell lines were confirmed upon storage at -20°C for 2, 6, and 12 months. The anti-HTLV-1/2 IgG1 reactivity, expressed as percentage of positive fluorescent cells (PPFC), was evaluated for each target antigen along the titration curve of test serum samples (1:32 to 1:4,096). Upon selection of target cell line and serum dilutions with higher segregation score between groups, the performance of "FIX" and "FIX & PERM" protocols was evaluated. The "FIX" protocol presented excellent performance indices (Se = 92%/Sp = 94%/AUC = 0.96; Se = 96%/Sp = 100%/AUC = 0.99) for the universal (HTLV-1/2 vs. NI) and differential (HTLV-1 vs. HTLV-2) diagnosis of HTLV-1 infection, respectively. Optimization of the "FIX" protocol using the principle of synchronous and asynchronous pairwise analysis further improved the performance of "FC-Duplex IgG1 (HTLV-1/2)", using the "FIX" protocol for differential diagnosis of HTLV-1 and HTLV-2 infections (Se = 100%/Sp = 100%/AUC = 1.00). In conclusion, the "FC-Duplex IgG1 (HTLV-1/2)" method represents an innovation in the biotechnology segment with the potential to compose a serological kit for differential diagnosis of HTLV-1/2 infection for reference laboratories and blood centers.HTLVHTLV-1/2Competitive assayFlow cytometryUniversal and differential diagnosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55682/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALA New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1_2 Infection - fimmu-13-795815.pdfA New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1_2 Infection - fimmu-13-795815.pdfapplication/pdf7634221https://www.arca.fiocruz.br/bitstream/icict/55682/2/A%20New%20Flow%20Cytometry-Based%20Single%20Platform%20for%20Universal%20and%20Differential%20Serodiagnosis%20of%20HTLV-1_2%20Infection%20-%20fimmu-13-795815.pdf8243194f81454f7d9279b382ad1505f1MD52icict/556822023-09-14 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| dc.title.en_US.fl_str_mv |
A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection |
| title |
A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection |
| spellingShingle |
A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection Paiva, Luciene Pimenta de HTLV HTLV-1/2 Competitive assay Flow cytometry Universal and differential diagnosis |
| title_short |
A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection |
| title_full |
A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection |
| title_fullStr |
A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection |
| title_full_unstemmed |
A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection |
| title_sort |
A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection |
| author |
Paiva, Luciene Pimenta de |
| author_facet |
Paiva, Luciene Pimenta de Reis, Jordana Grazziela Alves Coelho Dos Trindade, Bruno Caetano Pascoal, Vanessa Peruhype Magalhães Araújo, Márcio Sobreira Silva Gonçalves, Juan Jonathan Nogueira, Ana Caroline Martins, Júlia Pereira Ribeiro, Ágata Lopes Starling, Ana Lucia Alcântara, Luiz Carlos Júnior Ribeiro, Maísa Aparecida Proietti, Anna Bárbara de Freitas Carneiro Sabino, Ester Cerdeira Bicalho, Kelly Alves Carvalho, Andréa Teixeira Martins Filho, Olindo Assis |
| author_role |
author |
| author2 |
Reis, Jordana Grazziela Alves Coelho Dos Trindade, Bruno Caetano Pascoal, Vanessa Peruhype Magalhães Araújo, Márcio Sobreira Silva Gonçalves, Juan Jonathan Nogueira, Ana Caroline Martins, Júlia Pereira Ribeiro, Ágata Lopes Starling, Ana Lucia Alcântara, Luiz Carlos Júnior Ribeiro, Maísa Aparecida Proietti, Anna Bárbara de Freitas Carneiro Sabino, Ester Cerdeira Bicalho, Kelly Alves Carvalho, Andréa Teixeira Martins Filho, Olindo Assis |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Paiva, Luciene Pimenta de Reis, Jordana Grazziela Alves Coelho Dos Trindade, Bruno Caetano Pascoal, Vanessa Peruhype Magalhães Araújo, Márcio Sobreira Silva Gonçalves, Juan Jonathan Nogueira, Ana Caroline Martins, Júlia Pereira Ribeiro, Ágata Lopes Starling, Ana Lucia Alcântara, Luiz Carlos Júnior Ribeiro, Maísa Aparecida Proietti, Anna Bárbara de Freitas Carneiro Sabino, Ester Cerdeira Bicalho, Kelly Alves Carvalho, Andréa Teixeira Martins Filho, Olindo Assis |
| dc.subject.en.en_US.fl_str_mv |
HTLV HTLV-1/2 Competitive assay Flow cytometry Universal and differential diagnosis |
| topic |
HTLV HTLV-1/2 Competitive assay Flow cytometry Universal and differential diagnosis |
| description |
Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil. |
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2022 |
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2022-11-17T17:43:10Z |
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2022-11-17T17:43:10Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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PAIVA, Luciene Pimenta de et al. A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection. Frontiers in Immunology, v. 13, 795815, p. 1-15, Apr. 2022. |
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https://www.arca.fiocruz.br/handle/icict/55682 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2022.795815 |
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PAIVA, Luciene Pimenta de et al. A New Flow Cytometry-Based Single Platform for Universal and Differential Serodiagnosis of HTLV-1/2 Infection. Frontiers in Immunology, v. 13, 795815, p. 1-15, Apr. 2022. 1664-3224 10.3389/fimmu.2022.795815 |
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Frontiers Research Foundation |
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Frontiers Research Foundation |
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