Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replication

Detalhes bibliográficos
Autor(a) principal: Regis, Eduardo G.
Data de Publicação: 2010
Outros Autores: Souza, Victor Barreto de, Morgado, Mariza G., Bozza, Marcelo T., Leng, Lin, Bucala, Richard, Bou-Habib, Dumith C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17687
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
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spelling Regis, Eduardo G.Souza, Victor Barreto deMorgado, Mariza G.Bozza, Marcelo T.Leng, LinBucala, RichardBou-Habib, Dumith C.2017-02-07T10:49:09Z2017-02-07T10:49:09Z2010REGIS, Eduardo G. et al. Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: A relevant role on viral replication. Virology, v.399, n.1, p.31-38, Mar. 2010.0042-6822https://www.arca.fiocruz.br/handle/icict/1768710.1016/j.virol.2009.12.018engElsevierAIDSHIV-1MIFHIV-1AIDSLTRgp120Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replicationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ. Brasil.Universidade Federa do Rio de Janeiro. Instituto de Microbiologia. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Yale School of Medicine. CT, USA.Yale School of Medicine. CT, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.The cytokine macrophage migration inhibitory factor (MIF) is involved in the pathogenesis of inflammatory and infectious diseases, however its role in HIV-1 infection is unknown. Here we show that HIV-1-infected patients present elevated plasma levels of MIF, that HIV-1-infected peripheral blood mononuclear cells (PBMCs) release a greater amount of MIF, and that the HIV-1 envelope glycoprotein gp120 induces MIF secretion from uninfected PBMCs. The HIV-1 replication in PBMCs declines when these cells are treated with anti-MIF antibodies, and exposure of HIV-1-infected cells to the ABC-transporter inhibitor probenecid results in inhibition of MIF secretion. The addition of recombinant MIF (rhMIF) to HIV-1-infected PBMCs enhances viral replication of CCR5- or CXCR4-tropic HIV-1 isolates. Using a T CD4(+) cell lineage containing an HIV long terminal repeats (LTR)-Luciferase construct, we detected that rhMIF promotes transcription from HIV-1 LTR. Our results show that HIV-1 induces MIF secretion and suggest that MIF influences the HIV-1 biology through activation of HIV-1 LTR.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17687/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmariza_morgado_etal_IOC_2010.pdfmariza_morgado_etal_IOC_2010.pdfapplication/pdf422138https://www.arca.fiocruz.br/bitstream/icict/17687/2/mariza_morgado_etal_IOC_2010.pdf8cdbe078401f2b86d6bd231e3fa28602MD52TEXTmariza_morgado_etal_IOC_2010.pdf.txtmariza_morgado_etal_IOC_2010.pdf.txtExtracted texttext/plain50498https://www.arca.fiocruz.br/bitstream/icict/17687/3/mariza_morgado_etal_IOC_2010.pdf.txt220fef77783ac3393a990d8c3cec1cf9MD53icict/176872018-08-15 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dc.title.pt_BR.fl_str_mv Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replication
title Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replication
spellingShingle Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replication
Regis, Eduardo G.
AIDS
HIV-1
MIF
HIV-1
AIDS
LTR
gp120
title_short Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replication
title_full Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replication
title_fullStr Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replication
title_full_unstemmed Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replication
title_sort Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: a relevant role on viral replication
author Regis, Eduardo G.
author_facet Regis, Eduardo G.
Souza, Victor Barreto de
Morgado, Mariza G.
Bozza, Marcelo T.
Leng, Lin
Bucala, Richard
Bou-Habib, Dumith C.
author_role author
author2 Souza, Victor Barreto de
Morgado, Mariza G.
Bozza, Marcelo T.
Leng, Lin
Bucala, Richard
Bou-Habib, Dumith C.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Regis, Eduardo G.
Souza, Victor Barreto de
Morgado, Mariza G.
Bozza, Marcelo T.
Leng, Lin
Bucala, Richard
Bou-Habib, Dumith C.
dc.subject.other.pt_BR.fl_str_mv AIDS
HIV-1
topic AIDS
HIV-1
MIF
HIV-1
AIDS
LTR
gp120
dc.subject.en.pt_BR.fl_str_mv MIF
HIV-1
AIDS
LTR
gp120
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2017-02-07T10:49:09Z
dc.date.available.fl_str_mv 2017-02-07T10:49:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REGIS, Eduardo G. et al. Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: A relevant role on viral replication. Virology, v.399, n.1, p.31-38, Mar. 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17687
dc.identifier.issn.pt_BR.fl_str_mv 0042-6822
dc.identifier.doi.none.fl_str_mv 10.1016/j.virol.2009.12.018
identifier_str_mv REGIS, Eduardo G. et al. Elevated levels of macrophage migration inhibitory factor (MIF) in the plasma of HIV-1-infected patients and in HIV-1-infected cell cultures: A relevant role on viral replication. Virology, v.399, n.1, p.31-38, Mar. 2010.
0042-6822
10.1016/j.virol.2009.12.018
url https://www.arca.fiocruz.br/handle/icict/17687
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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