Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Saldanha, Maíra Garcia
Data de Publicação: 2017
Outros Autores: Queiroz, Adriano, Machado, Paulo Roberto Lima, Carvalho, Lucas Pedreira de, Scott, Phillip, Carvalho Filho, Edgar Marcelino, Arruda, Sérgio Marcos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18978
Resumo: National Institutes of Health (grant AI088650) and Conselho Nacional de Pesquisa Instituto Nacional de Ciência e Tecnologia—Doenças Tropicais (grant 573839/2008-5).
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spelling Saldanha, Maíra GarciaQueiroz, AdrianoMachado, Paulo Roberto LimaCarvalho, Lucas Pedreira deScott, PhillipCarvalho Filho, Edgar MarcelinoArruda, Sérgio Marcos2017-05-19T13:35:55Z2017-05-19T13:35:55Z2017SALDANHA, M. G. et al. Characterization of the histopathologic features in patients in the early and late phases of Cutaneous Leishmaniasis. American Journal of Tropical Medicine and Hygiene, v. 96, n. 3, p. 645–652, 2017.0002-9637https://www.arca.fiocruz.br/handle/icict/1897810.4269/ajtmh.16-0539National Institutes of Health (grant AI088650) and Conselho Nacional de Pesquisa Instituto Nacional de Ciência e Tecnologia—Doenças Tropicais (grant 573839/2008-5).2018-01-23Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversity of Pennsylvania. School of Veterinary Medicine. Department of Pathobiology. Philadelphia, PennsylvaniaFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Complexo Hospitalar Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilCutaneous leishmaniasis (CL), characterized by an ulcerated lesion, is the most common clinical form of human leishmaniasis. Before the ulcer develops, patients infected with Leishmania (Viannia) braziliensis present a small papule at the site of the sandfly bite, referred to as early cutaneous leishmaniasis (E-CL). Two to four weeks later the typical ulcer develops, which is considered here as late CL (L-CL). Although there is a great deal known about T-cell responses in patients with L-CL, there is little information about the in situ inflammatory response in E-CL. Histological sections of skin biopsies from 15 E-CL and 28 L-CL patients were stained by hematoxilin and eosin to measure the area infiltrated by cells, as well as tissue necrosis. Leishmania braziliensis amastigotes, CD4(+), CD8(+), CD20(+), and CD68(+) cells were identified and quantified by immunohistochemistry. The number of amastigotes in E-CL was higher than in L-CL, and the inflammation area was larger in classical ulcers than in E-CL. There was no relationship between the number of parasites and magnitude of the inflammation area, or with the lesion size. However, there was a direct correlation between the number of macrophages and the lesion size in E-CL, and between the number of macrophages and necrotic area throughout the course of the disease. These positive correlations suggest that macrophages are directly involved in the pathology of L. braziliensis-induced lesions.engAmerican Society of Tropical Medicine and HygieneLeishmaniose cutâneaLeishmania (Viannia) braziliensisHistopatologiaBiopsiaÚlcera cutâneaMacrófagosCutaneous LeishmaniasisLeishmania (Viannia) braziliensisHistopathologyBiopsiesCutaneous ulcerMacrophagesLeishmaniose cutâneaLeishmania (Viannia) braziliensisHistopatologiaBiópsiaÚlcera cutâneaMacrófagosCharacterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18978/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSaldanha and characterization 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dc.title.pt_BR.fl_str_mv Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis
title Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis
spellingShingle Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis
Saldanha, Maíra Garcia
Leishmaniose cutânea
Leishmania (Viannia) braziliensis
Histopatologia
Biopsia
Úlcera cutânea
Macrófagos
Cutaneous Leishmaniasis
Leishmania (Viannia) braziliensis
Histopathology
Biopsies
Cutaneous ulcer
Macrophages
Leishmaniose cutânea
Leishmania (Viannia) braziliensis
Histopatologia
Biópsia
Úlcera cutânea
Macrófagos
title_short Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis
title_full Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis
title_fullStr Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis
title_full_unstemmed Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis
title_sort Characterization of the Histopathologic Features in Patients in the Early and Late Phases of Cutaneous Leishmaniasis
author Saldanha, Maíra Garcia
author_facet Saldanha, Maíra Garcia
Queiroz, Adriano
Machado, Paulo Roberto Lima
Carvalho, Lucas Pedreira de
Scott, Phillip
Carvalho Filho, Edgar Marcelino
Arruda, Sérgio Marcos
author_role author
author2 Queiroz, Adriano
Machado, Paulo Roberto Lima
Carvalho, Lucas Pedreira de
Scott, Phillip
Carvalho Filho, Edgar Marcelino
Arruda, Sérgio Marcos
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Saldanha, Maíra Garcia
Queiroz, Adriano
Machado, Paulo Roberto Lima
Carvalho, Lucas Pedreira de
Scott, Phillip
Carvalho Filho, Edgar Marcelino
Arruda, Sérgio Marcos
dc.subject.other.pt_BR.fl_str_mv Leishmaniose cutânea
Leishmania (Viannia) braziliensis
Histopatologia
Biopsia
Úlcera cutânea
Macrófagos
topic Leishmaniose cutânea
Leishmania (Viannia) braziliensis
Histopatologia
Biopsia
Úlcera cutânea
Macrófagos
Cutaneous Leishmaniasis
Leishmania (Viannia) braziliensis
Histopathology
Biopsies
Cutaneous ulcer
Macrophages
Leishmaniose cutânea
Leishmania (Viannia) braziliensis
Histopatologia
Biópsia
Úlcera cutânea
Macrófagos
dc.subject.en.pt_BR.fl_str_mv Cutaneous Leishmaniasis
Leishmania (Viannia) braziliensis
Histopathology
Biopsies
Cutaneous ulcer
Macrophages
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose cutânea
Leishmania (Viannia) braziliensis
Histopatologia
Biópsia
Úlcera cutânea
Macrófagos
description National Institutes of Health (grant AI088650) and Conselho Nacional de Pesquisa Instituto Nacional de Ciência e Tecnologia—Doenças Tropicais (grant 573839/2008-5).
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-05-19T13:35:55Z
dc.date.available.fl_str_mv 2017-05-19T13:35:55Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SALDANHA, M. G. et al. Characterization of the histopathologic features in patients in the early and late phases of Cutaneous Leishmaniasis. American Journal of Tropical Medicine and Hygiene, v. 96, n. 3, p. 645–652, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18978
dc.identifier.issn.pt_BR.fl_str_mv 0002-9637
dc.identifier.doi.none.fl_str_mv 10.4269/ajtmh.16-0539
identifier_str_mv SALDANHA, M. G. et al. Characterization of the histopathologic features in patients in the early and late phases of Cutaneous Leishmaniasis. American Journal of Tropical Medicine and Hygiene, v. 96, n. 3, p. 645–652, 2017.
0002-9637
10.4269/ajtmh.16-0539
url https://www.arca.fiocruz.br/handle/icict/18978
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
publisher.none.fl_str_mv American Society of Tropical Medicine and Hygiene
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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