SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics

Detalhes bibliográficos
Autor(a) principal: Greenwood, J.
Data de Publicação: 1996
Outros Autores: Pryce, G., Devine, L., Male, D. K., Santos, Washington Luis Conrado dos, Calder, V. L., Adamson, P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20037
Resumo: dosSantos, Washington Luis Conrado “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
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spelling Greenwood, J.Pryce, G.Devine, L.Male, D. K.Santos, Washington Luis Conrado dosCalder, V. L.Adamson, P.2017-07-11T13:03:00Z2017-07-11T13:03:00Z1996GREENWOOD, J. et al. SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics. Journal of Neuroimmunology, v. 71, p. 51-63, 1996.0165-5728https://www.arca.fiocruz.br/handle/icict/20037dosSantos, Washington Luis Conrado “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.University College London. Institute of Ophthalmology. Department of Clinical Ophthalmology. London, UKUniversity College London. Institute of Ophthalmology. Department of Clinical Ophthalmology. London, UKUniversity College London. Institute of Ophthalmology. Department of Clinical Ophthalmology. London, UKInstitute of Psychiatry. Department of Neuropathology. London, UKInstitute of Psychiatry. Department of Neuropathology. London, UKUniversity College London. Institute of Ophthalmology. Department of Clinical Ophthalmology. London, UKUniversity College London. Institute of Ophthalmology. Department of Clinical Ophthalmology. London, UKIn the central nervous system the blood–brain and blood–retinal barriers (BBBand BRBrespectively)are instrumentalin maintaining homeostasisof the neural parenchymaand controllingleucocytetraffic. These cellular barriers are formed primarilyby the vascular endotheliumof the brain and retina althoughin the latter the pigmentedepithelial cells also form part of the barrier. From primary culturesof rat brain endothelium,retinal endotheliumand retinal pigmentepitheiium(RPE) we have generatedtemperaturesensitive SV40largeT immortalisedcell lines. Clonesof brain (GP8.3)and retinal(JG2.1)endotheliaand RPE (LD7.4)have been derivedfrom parent lines that expressthe large T antigen at the permissivetemperature.The endothelialcell (EC) lines expressedP-glycoprotein, GLUT-1,the transfernnreceptor,von Willebrandfactor and the RECA-1antigenand exhibitedhigh affinityuptakeof acetylatedLDL and stainedpositivewith the lectin Griffoniasimplicifolia.The RPE cell line was positivefor cytokeratinsand for the rat RPE antigen RET-PE2.All the cell lines expressedmajor histocompatibilitycomplex(MHC)class 1 and intercellularadhesionmolecule(ICAM)-1 constitutivelyand could be induced to expressMHC class II and vascular cell adhesion molecule (VCAM)-1following cytokine activation.The EC also expressedplatelet endothelialcelI adhesionmolecule(PECAM)-I.Monolayer of these cells could supportthe migrationof antigen-specificT cell lines.Thegenerationof immortalisedcell linesderivedfromthe rat BBBandBRBshouldproveto be usefultools for the studyof these specialisedcellularbarriers.engElsevierBarreira hematoencefalicaBarreira sangue-retinaEndotélioEpitélio pigmentado da retinaBlood–brain barrierBlood–retinal barrierEndotheliumImmortalisationRetinal pigment epitheliumsSV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristicsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20037/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGreenwood J Sv40 Large T Cell....pdfGreenwood J Sv40 Large T Cell....pdfapplication/pdf2977496https://www.arca.fiocruz.br/bitstream/icict/20037/2/Greenwood%20J%20Sv40%20Large%20T%20Cell....pdfef3dbfdbc9a0e87c2ad7e0fcd9219611MD52TEXTGreenwood J Sv40 Large T Cell....pdf.txtGreenwood J Sv40 Large T Cell....pdf.txtExtracted texttext/plain52811https://www.arca.fiocruz.br/bitstream/icict/20037/3/Greenwood%20J%20Sv40%20Large%20T%20Cell....pdf.txt60071a4bcf41347ababef367de1286f1MD53icict/200372023-03-15 14:32:48.903oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:48Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics
title SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics
spellingShingle SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics
Greenwood, J.
Barreira hematoencefalica
Barreira sangue-retina
Endotélio
Epitélio pigmentado da retina
Blood–brain barrier
Blood–retinal barrier
Endothelium
Immortalisation
Retinal pigment epitheliums
title_short SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics
title_full SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics
title_fullStr SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics
title_full_unstemmed SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics
title_sort SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics
author Greenwood, J.
author_facet Greenwood, J.
Pryce, G.
Devine, L.
Male, D. K.
Santos, Washington Luis Conrado dos
Calder, V. L.
Adamson, P.
author_role author
author2 Pryce, G.
Devine, L.
Male, D. K.
Santos, Washington Luis Conrado dos
Calder, V. L.
Adamson, P.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Greenwood, J.
Pryce, G.
Devine, L.
Male, D. K.
Santos, Washington Luis Conrado dos
Calder, V. L.
Adamson, P.
dc.subject.other.pt_BR.fl_str_mv Barreira hematoencefalica
Barreira sangue-retina
Endotélio
Epitélio pigmentado da retina
topic Barreira hematoencefalica
Barreira sangue-retina
Endotélio
Epitélio pigmentado da retina
Blood–brain barrier
Blood–retinal barrier
Endothelium
Immortalisation
Retinal pigment epitheliums
dc.subject.en.pt_BR.fl_str_mv Blood–brain barrier
Blood–retinal barrier
Endothelium
Immortalisation
Retinal pigment epitheliums
description dosSantos, Washington Luis Conrado “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.
publishDate 1996
dc.date.issued.fl_str_mv 1996
dc.date.accessioned.fl_str_mv 2017-07-11T13:03:00Z
dc.date.available.fl_str_mv 2017-07-11T13:03:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GREENWOOD, J. et al. SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics. Journal of Neuroimmunology, v. 71, p. 51-63, 1996.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20037
dc.identifier.issn.pt_BR.fl_str_mv 0165-5728
identifier_str_mv GREENWOOD, J. et al. SV40 large T immortalised cell lines of the rat blood–brain and blood–retinal barriers retain their phenotypic and immunological characteristics. Journal of Neuroimmunology, v. 71, p. 51-63, 1996.
0165-5728
url https://www.arca.fiocruz.br/handle/icict/20037
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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