RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia.

Detalhes bibliográficos
Autor(a) principal: Xavier, Marcelo Antônio Pascoal
Data de Publicação: 2015
Outros Autores: Figueiredo, Anna Carolina Cançado, Gomes, Luciana Inácia, Pascoal, Vanessa Peruhype Magalhães, Silva, Carlos Eduardo Calzavara, Costa, Marcelo Azevedo, Reis, Ilka Afonso, Bonjardim, Claudio Antônio, Kroon, Erna Geessien, Oliveira, Jaquelline Germano de, Ferreira, Paulo César Peregrino
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12624
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil
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spelling Xavier, Marcelo Antônio PascoalFigueiredo, Anna Carolina CançadoGomes, Luciana InáciaPascoal, Vanessa Peruhype MagalhãesSilva, Carlos Eduardo CalzavaraCosta, Marcelo AzevedoReis, Ilka AfonsoBonjardim, Claudio AntônioKroon, Erna GeessienOliveira, Jaquelline Germano deFerreira, Paulo César Peregrino2016-01-26T15:04:58Z2016-01-26T15:04:58Z2015XAVIER, Marcelo Antonio Pascoal et al. RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia. PLoS One, vol. 10, n. 4, p. e0123531, 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1262410.1371/journal.pone.0123531. eCollection 2015.engPublic Library of ScienceRAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte MG, BrasilUniversidade Federal de Minas Gerais. Escola de Engenharia. Departamento de Engenharia de Produção. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Exatas. Departamento de Estatística. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilRAP1 (RAS proximate 1), a small GTP-binding protein of the RAS superfamily, is a putative oncogene that is highly expressed in several malignant cell lines and types of cancers, including some types of squamous cell carcinoma. However, the participation of RAP1 in cervical carcinogenesis is unknown. We conducted a cross-sectional study of paraffin-embedded cervical biopsies to determine the association of RAP1 with cervical intraepithelial neoplasia (CIN). Standard and quantitative immunohistochemistry assessment of RAP1 expression in fixed tissue was performed on 183 paraffin-embedded cervical biopsies that were classified as normal or non-dysplastic mucosa (NDM) (n = 33); CIN grade 1 (n = 84) and CIN grade 2/3 (n = 66). A gradual increase in RAP1 expression in NDM < CIN 1 < CIN 2/3 (p<0.001) specimens was observed and was in agreement with the histopathologic diagnosis. A progressive increase in the RAP1 expression levels increased the risk of CIN 1 [odds ratio (OR) = 3.50; 95% confidence interval (CI) 1.30-10.64] 3.5 fold and the risk of CIN 2/3 (OR = 19.86, 95% CI 6.40-70.79) nearly 20 fold when compared to NDM. In addition, stereotype ordinal regression analysis showed that this progressive increase in RAP1 expression more strongly impacted CIN 2/3 than CIN 1. Our findings suggest that RAP1 may be a useful biomarker for the diagnosis of CINCell stainingImmunostainingBiomarkersNuclear stainingEpitheliumMorphometryBiopsyCarcinogenesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12624/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia .pdfRAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia .pdfapplication/pdf3813372https://www.arca.fiocruz.br/bitstream/icict/12624/2/RAP1%20GTPase%20Overexpression%20is%20Associated%20with%20Cervical%20Intraepithelial%20Neoplasia%20.pdf8d45353e084f4f7c3069a1cc6c773c95MD52TEXTRAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia .pdf.txtRAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia .pdf.txtExtracted texttext/plain774https://www.arca.fiocruz.br/bitstream/icict/12624/3/RAP1%20GTPase%20Overexpression%20is%20Associated%20with%20Cervical%20Intraepithelial%20Neoplasia%20.pdf.txt9f8acc1f7254ffb0fc69483d5a78797bMD53icict/126242019-06-19 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dc.title.pt_BR.fl_str_mv RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia.
title RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia.
spellingShingle RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia.
Xavier, Marcelo Antônio Pascoal
Cell staining
Immunostaining
Biomarkers
Nuclear staining
Epithelium
Morphometry
Biopsy
Carcinogenesis
title_short RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia.
title_full RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia.
title_fullStr RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia.
title_full_unstemmed RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia.
title_sort RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia.
author Xavier, Marcelo Antônio Pascoal
author_facet Xavier, Marcelo Antônio Pascoal
Figueiredo, Anna Carolina Cançado
Gomes, Luciana Inácia
Pascoal, Vanessa Peruhype Magalhães
Silva, Carlos Eduardo Calzavara
Costa, Marcelo Azevedo
Reis, Ilka Afonso
Bonjardim, Claudio Antônio
Kroon, Erna Geessien
Oliveira, Jaquelline Germano de
Ferreira, Paulo César Peregrino
author_role author
author2 Figueiredo, Anna Carolina Cançado
Gomes, Luciana Inácia
Pascoal, Vanessa Peruhype Magalhães
Silva, Carlos Eduardo Calzavara
Costa, Marcelo Azevedo
Reis, Ilka Afonso
Bonjardim, Claudio Antônio
Kroon, Erna Geessien
Oliveira, Jaquelline Germano de
Ferreira, Paulo César Peregrino
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Xavier, Marcelo Antônio Pascoal
Figueiredo, Anna Carolina Cançado
Gomes, Luciana Inácia
Pascoal, Vanessa Peruhype Magalhães
Silva, Carlos Eduardo Calzavara
Costa, Marcelo Azevedo
Reis, Ilka Afonso
Bonjardim, Claudio Antônio
Kroon, Erna Geessien
Oliveira, Jaquelline Germano de
Ferreira, Paulo César Peregrino
dc.subject.en.pt_BR.fl_str_mv Cell staining
Immunostaining
Biomarkers
Nuclear staining
Epithelium
Morphometry
Biopsy
Carcinogenesis
topic Cell staining
Immunostaining
Biomarkers
Nuclear staining
Epithelium
Morphometry
Biopsy
Carcinogenesis
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-26T15:04:58Z
dc.date.available.fl_str_mv 2016-01-26T15:04:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv XAVIER, Marcelo Antonio Pascoal et al. RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia. PLoS One, vol. 10, n. 4, p. e0123531, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12624
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0123531. eCollection 2015.
identifier_str_mv XAVIER, Marcelo Antonio Pascoal et al. RAP1 GTPase Overexpression is Associated with Cervical Intraepithelial Neoplasia. PLoS One, vol. 10, n. 4, p. e0123531, 2015.
1932-6203
10.1371/journal.pone.0123531. eCollection 2015.
url https://www.arca.fiocruz.br/handle/icict/12624
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12624/1/license.txt
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https://www.arca.fiocruz.br/bitstream/icict/12624/3/RAP1%20GTPase%20Overexpression%20is%20Associated%20with%20Cervical%20Intraepithelial%20Neoplasia%20.pdf.txt
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